Molecular and morphological identification of Biomphalaria species from the state of São Paulo, Brazil

Palasio, Raquel Gardini Sanches; Guimarães, Marisa Cristina de Almeida; Ohlweiler, Fernanda Pires; Tuan, Roseli

doi:10.3897/zookeys.668.10562

Cited by 25 publications

(16 citation statements)

References 48 publications

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“…Results obtained for species of freshwater and land mollusks which are medically relevant in the neotropics show the importance of combining traditional methods of morphological analysis with molecular methods to identify known species as well as potential cryptic species (Langand et al 1999, Campbell et al 2000, Dejong et al 2003, Wethington et al 2007, Teodoro et al 2010, Tuan et al 2012, Palasio et al 2017, Bezerra et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Diversity of Biomphalaria spp. freshwater snails and associated mollusks in areas with schistosomiasis risk, using molecular and spatial analysis tools

et al. 2019

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The Middle Paranapanema River region of São Paulo, Brazil is home to significant diversity of Biomphalaria species and is very vulnerable to health and environmental impacts such as schistosomiasis. This study updates freshwater malacological surveys for ecosystems in one portion of the Middle Paranapanema River Basin, with emphasis on the genus Biomphalaria. Snails were collected from 114 distinct bodies of water between 2015 and 2018. Biomphalaria specimens were identified according to morphological and molecular characteristics, while animals in other genera (Drepanotrema, Lymnaea, Melanoides, Physa and Pomacea) were identified solely according to shell characteristics. A geographic information system was used to update intermediate host colonization sites and consequently assist in identifying probable hotspots for intermediate hosts of schistosomiasis. The sequences of the COI gene relating to the DNA barcode stretch were tested for similarity against sequences found in GenBank, for monophyly through Maximum Likelihood phylogenetic inference, and analyzed in ABDG, bPTP and GMYC for the delimitation of putative species. Of the 10,722 snails collected, 86.7% were in the Planorbidae family (75.5% Biomphalaria and 11.2% Drepanotrema) and 13.3% were other non-Planorbidae species (Lymnaea, Melanoides, Physa and Pomacea). The taxonomic COI reference sequences in the NCBI nucleotide database used for DNA sequence comparison, and phylogenetic analysis used to test the monophyly of the groups, resulted in more reliable taxonomic units than delimitation of the COI sequences in MOTUs using statistical taxonomic models. Analysis of the species distribution shows that B. glabrata and B. tenagophila are heterogeneously distributed in the study area. B. glabrata colonizes only five water bodies, in the study area, most of them in Ourinhos, while B. tenagophila predominates in water bodies in Ipaussu. Contrasting with this, B. straminea, B. occidentalis and B. peregrina are evenly distributed throughout the study area.

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Section: Introductionmentioning

confidence: 99%

Diversity of Biomphalaria spp. freshwater snails and associated mollusks in areas with schistosomiasis risk, using molecular and spatial analysis tools

et al. 2019

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“…Identification of Biomphalaria species is challenging because shell features are often highly variable and can even be confused with shells of other genera like Helisoma 44 . Anatomical characteristics, often revealed only by painstaking dissections of specially prepared materials, require experience, patience and practice to discern 13 , 45 , 46 . Nonetheless, reference to both shell and anatomical features in conjunction with provision of an ever-increasing array of molecular data has provided an improved and workable overall systematic framework for Biomphalaria .…”

Section: Discussionmentioning

confidence: 99%

Complete mitochondrial and rDNA complex sequences of important vector species of Biomphalaria, obligatory hosts of the human-infecting blood fluke, Schistosoma mansoni

Zhang

Laidemitt

et al. 2018

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Using high throughput Illumina sequencing technology, we determined complete sequences for the mitochondrial genome (mitogenome) and nuclear ribosomal DNA (rDNA) complex for three African freshwater snail taxa within the genus Biomphalaria, B. pfeifferi, B. sudanica and B. choanomphala, and for two laboratory strains of B. glabrata originating from the Neotropics. Biomphalaria snails are obligate vectors of the blood fluke Schistosoma mansoni, a major etiologic agent of human intestinal schistosomiasis. Our data show that mitogenomes from African and Neotropical Biomphalaria are highly conserved. With respect to rDNA, the two internal transcribed spacers (ITS1 and 2) were found to be highly variable whereas the three ribosomal RNA genes (28S, 5.8S and 18S rRNA) exhibited no or very limited variation. Our analyses reveal that the two taxa inhabiting Lake Victoria, B. sudanica and B. choanomphala, are very similar to one another relative to the similarity either shows to B. pfeifferi or B. glabrata. This new sequence information may prove useful for developing new markers for snail identification, environmental detection/monitoring purposes or for tracking epidemiology and snail dependencies of S. mansoni in endemic areas. It also provides new information pertinent to still unresolved questions in Biomphalaria systematics and nomenclature.

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“…Part of the COI gene (~600 bp) was amplified with the LCO1490/HCO2198 primers (FolMeR et al 1994) (2007). All polymerase chain reactions (PCR) were carried out in a total volume of 50 µl as described in PalaSio et al (2017). The amplified ITS2 fragments were cloned using pGEM-T (Promega).…”

Section: Dna Extraction Pcr Amplifications Genetic Population Strumentioning

confidence: 99%

Genetic diversity of the freshwater snail Biomphalaria tenagophila (d’Orbigny, 1835) (Gastropoda: Hygrophila: Planorbidae) across two coastal areas of southeast Brazil

Palasio¹,

Zanotti-Magalhães²,

Tuan³

2018

Folia Malacol.

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This study uses two mitochondrial (COI, 16SrRNA) and one nuclear marker (ITS2) to explore genetic diversity between populations of B. tenagophila (d'Orbigny), the most common intermediate host for Schistosoma mansoni in southeast Brazil. The snails were collected in freshwater bodies along the southern coastal region of Ribeira do Iguape river basin and along the northern coastal region of the state of São Paulo, Brazil. The estimates of genetic differentiation between the populations (Φ) indicate that there is no genetic isolation between northern and southern populations. The haplotype diversity observed in B. tenagophila shows a complex pattern of distribution: in the southern region, the genetic variation in B. tenagophila is partitioned into small and more heterogeneous mitochondrial haplotypes, whereas in the northern region there were one to three homogeneous haplotypes. The results may suggest a different colonisation history across the study area.

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Molecular and morphological identification of Biomphalaria species from the state of São Paulo, Brazil

Cited by 25 publications

References 48 publications

Diversity of Biomphalaria spp. freshwater snails and associated mollusks in areas with schistosomiasis risk, using molecular and spatial analysis tools

Diversity of Biomphalaria spp. freshwater snails and associated mollusks in areas with schistosomiasis risk, using molecular and spatial analysis tools

Complete mitochondrial and rDNA complex sequences of important vector species of Biomphalaria, obligatory hosts of the human-infecting blood fluke, Schistosoma mansoni

Genetic diversity of the freshwater snail Biomphalaria tenagophila (d’Orbigny, 1835) (Gastropoda: Hygrophila: Planorbidae) across two coastal areas of southeast Brazil

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