2004
DOI: 10.1128/jcm.42.6.2450-2454.2004
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Molecular and Morphological Identification ofColletotrichumSpecies of Clinical Interest

Abstract: Colletotrichum species have caused human infections in recent years. Because of the difficulties in recognizing them in vitro, we have designed a quick and unambiguous molecular test, based on the amplification of a specific fragment of the internal transcribed spacer 1 region, to distinguish any Colletotrichum isolate from other fungi, including the common pathogenic species. Analysis of the sequences of the ribosomal DNA (rDNA) fragment showed sufficient variability to clearly separate the five species of Co… Show more

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Cited by 120 publications
(85 citation statements)
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“…The aetiological characterisation was possible by molecular biology (polymerase chain reaction) only, as it was not possible to conduct a microscopic examination of the fungal colonies. However, in recent years, five species of the Colletotrichum genus have been reported as agents of human infections (Cano et al 2004).…”
Section: Discussionmentioning
confidence: 99%
“…The aetiological characterisation was possible by molecular biology (polymerase chain reaction) only, as it was not possible to conduct a microscopic examination of the fungal colonies. However, in recent years, five species of the Colletotrichum genus have been reported as agents of human infections (Cano et al 2004).…”
Section: Discussionmentioning
confidence: 99%
“…Las etapas de desarrollo de las especies de Colletotrichum pueden separarse en: 1) deposición en las superficie del hospedante, 2) fijación de la conidia en la superficie, 3) germinación de la conidia, 4) producción del apresorio, 5) penetración de la epidermis de la planta, 6) crecimiento y colonización del tejido del hospedante y 7) producción de acérvulos y esporulación (RODRÍGUEZ-LÓPEZ et al, 2009). Las conidias son variables en tamaño y su forma es cilíndrica con puntas redondeadas (CANO et al, 2004). El hongo produce apresorios melanizados con dimensiones variables (de 6 a 20 x 4 a 12 µm) a partir del cual se produce una púa de infección que penetra al fruto al degradar la cutícula mediante enzimas degradadoras de la pared celular y presión de turgencia, permaneciendo latente hasta que el fruto madura (BENO;PRUSKY, 2000).…”
Section: Análisis De La Infección De Colletotrichum Gloeosporioides (unclassified
“…Furthermore, Colletotrichum sp. have recently been reported to cause subcutaneous and systemic infections among immunosuppressed patients (2). Colletotrichum sp.…”
mentioning
confidence: 99%