Molecular and morphometric identification of Trypanosoma (Megatrypanum) minasense in blood samples of marmosets (Callithrix: Callithrichidae) from the city of Rio de Janeiro, Brazil.

Coimbra, Diogo P.; Penedo, Diego M.; Silva, Monique O.M.; Abreu, Ana Paula Martinez de; Silva, Cláudia Bezerra da; Verona, Carlos E.; Heliodoro, Gabriela; Massard, Carlos Luiz; Nogueira, Denise Monnerat

doi:10.1016/j.parint.2019.101999

Cited by 6 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the probability of finding infectious forms on light microscopy may be influenced by the low parasitemia in infections by Trypanosoma spp. (Ziccardi & Lourenço-de-Oliveira 1999, Tenório et al 2014, Coimbra et al 2019, while PCR is considered a more accurate diagnostic methodology (Tenório et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…In wild individuals of Callithrix sp. from Jardim Botânico, Rio de Janeiro, hemoparasites identified as T. minasense were found in 33% of the animals through morphometric data and in 20% of them based on DNA sequence similarity, also exhibiting size polymorphism (Coimbra et al 2019). The infection rate was similar to that found in the present study (19.2% for Sapajus sp.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular detection and phylogenetic analysis of Trypanosoma spp. in Neotropical primates from Rio de Janeiro State, Brazil

et al. 2022

View full text Add to dashboard Cite

Trypanosoma spp. infection is a problem in many tropical countries, infecting several animal species, including humans. The aim of the present study was to identify the Trypanosoma species in Neotropical primates from Rio de Janeiro state and compare the results with other reports both phylogenetically and geographically. Molecular detection was based on the 18 SSU gene. The sequences obtained in the PCR were sequenced and compared with others previously deposited in GenBank. These sequences were used to perform phylogenetic analysis and make a distribution map of primate species infected by Trypanosoma species in Brazil. Among 34 monkeys, five capuchin monkeys (Sapajus spp.) and one marmoset (Callithrix spp.) showed Trypanosoma spp. sequences in the same clade of Trypanosoma minasense and three capuchin monkeys’ sequences were in the same clade of Trypanosoma cruzi. The Atlantic Forest and the Brazilian Amazon are the regions with the highest frequency of studies about Trypanosoma spp. and variety of Neotropical primate hosts. These are areas that deserve attention regarding the conservation of biodiversity, but it also makes evident the lack of studies with Neotropical primates in other regions of the country, as well as multidisciplinary studies to better understand the host pathogen relationships.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular detection and phylogenetic analysis of Trypanosoma spp. in Neotropical primates from Rio de Janeiro State, Brazil

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Moreover, T. minasense has been detected in Alouatta caraya in captivity in Ilha Solteira, São Paulo, Brazil (Tenório et al, 2014), and three free-living Callithrix spp. at a botanical garden in downtown Rio de Janeiro, Brazil (Coimbra et al, 2020). Even with T. minasense present in several Brazilian regions, the public health risk is minimal since this protozoan is considered apathogenic; however, the reported finding is of descriptive importance.…”

mentioning

confidence: 96%

“…In Brazil, information regarding the potential role of primates as a reservoir of trypanosomatid infections is scarce, and diagnosis may represent a prophylactic measure for zoonoses control, especially for non-human primates because of their physiological, genetic, and geographic proximity to humans (Wolfe et al, 1998;Coimbra et al, 2020).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Molecular detection of trypanosomatids in neotropical primates in the state of Mato Grosso, Midwest, Brazil

Cândido

Pavelegini

Pacheco

et al. 2021

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Trypanosomatids are uniflagellate protozoa belonging to the Trypanosomatidae family. The genera Trypanosoma and Leishmania are of paramount importance as they contain species that cause serious diseases, such as Chagas disease and Leishmaniasis, respectively. The objective of the present study was to identify trypanosomatids present in the whole blood of free-living and captive neotropical primates in Mato Grosso State, Midwest Brazil. Between 2017 and 2019, 38 blood samples were collected from seven different neotropical primate species in seven cities in the state. Through molecular techniques, including polymerase chain reaction (PCR) to amplify a fragment of the kinetoplast DNA (kDNA) and 18S ribosomal RNA (18S rRNA) gene, sequencing, and phylogenetic analysis, nine Leishmania spp. [seven L. infantum and two L. (Leishmania) amazonensis] and two Trypanosoma spp. (T. minasense and T. rangeli) were identified. This study contributes to understanding the occurrence and epidemiology of trypanosomatids in Mato Grosso State and the importance of neotropical primates as trypanosome hosts and possible infection sources for other animals and humans. Future identification of other blood pathogens in neotropical primates will assist in disease control and prevention strategies.

show abstract

Serosurvey of Trypanosomatidae (Trypanosoma cruzi and Leishmania infantum) in Howler Monkeys from Southern Brazil: Risk Factor and Influence of Landscape Structure

et al. 2023

View full text Add to dashboard Cite

Molecular and morphometric identification of Trypanosoma (Megatrypanum) minasense in blood samples of marmosets (Callithrix: Callithrichidae) from the city of Rio de Janeiro, Brazil.

Cited by 6 publications

References 10 publications

Molecular detection and phylogenetic analysis of Trypanosoma spp. in Neotropical primates from Rio de Janeiro State, Brazil

Molecular detection and phylogenetic analysis of Trypanosoma spp. in Neotropical primates from Rio de Janeiro State, Brazil

Molecular detection of trypanosomatids in neotropical primates in the state of Mato Grosso, Midwest, Brazil

Serosurvey of Trypanosomatidae (Trypanosoma cruzi and Leishmania infantum) in Howler Monkeys from Southern Brazil: Risk Factor and Influence of Landscape Structure

Contact Info

Product

Resources

About