Molecular and phenotypic analyses reveal the non-identity of the Phaeobacter gallaeciensis type strain deposits CIP 105210T and DSM 17395

Abstract

The marine genus Phaeobacter currently comprises six species, some of which were intensively studied mainly due to their ability to produce secondary metabolites. The type strain of the type species, Phaeobacter gallaeciensis BS107 T , has been deposited at several public culture collections worldwide. Based on differences in plasmid profiles, we detected that the alleged P. gallaeciensis type strains deposited at the Collection Institute Pasteur (CIP; Paris, France) as CIP 105210 and at the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany) as DSM 17395 are not identical. To determine the identity of these strains, we conducted DNA-DNA hybridization, matrix-assisted laser desorption/ionization timeof-flight mass spectrometry (MALDI-TOF), 16S rRNA gene and internal transcribed spacer (ITS) sequence analyses, as well as physiological experiments. Based on the detailed 16S rRNA gene reanalysis we showed that strain CIP 105210 most likely corresponds to the original P. gallaeciensis type strain BS107 T . In contrast, the Phaeobacter strain DSM 17395 exhibits a much closer affiliation to Phaeobacter inhibens DSM 16374 T (5T5 T ) and should thus be allocated to this species. The detection of the dissimilarity of strains CIP 105210 T and DSM 17395 will influence future comparative studies within the genus Phaeobacter.

Effect of TDA‐producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non‐axenic algae and copepod systems

“…The highly virulent V. anguillarum strain 90‐11‐286 (Rønneseth et al ., 2017) was used in the co‐culture experiments and was isolated from diseased rainbow trout ( Oncorhynchus mykiss ) from a Danish aquaculture (Pedersen and Larsen, 1993; Skov et al ., 1995). P. inhibens DSM 17395 was used for the co‐culture experiments (Martens et al ., 2006; Vandecandelaere et al ., 2008; Buddruhs et al ., 2013). NB10 and 90‐11‐286 were grown and counted on TSA (Tryptone Soy Agar, Difco 212185) supplemented with 2.5 mg l −1 chloramphenicol for NB10.…”

Influence of Iron on Production of the Antibacterial Compound Tropodithietic Acid and Its Noninhibitory Analog in Phaeobacter inhibens

“…Phaeobacter inhibens DSM 17395 (25,26), its TDAnegative mutants MJG-G6 (tdaB::EZ-Tn5) (21) and DSM 17395-Pda8 (13), Phaeobacter sp. strain 27-4 (10), its TDA-negative mutant Phaeobacter sp.…”

Gene Regulatory and Metabolic Adaptation Processes of Dinoroseobacter shibae DFL12T during Oxygen Depletion

“…Until now, our knowledge about the details of the metabolic processes in Roseobacter clade members has been rather limited. Recently, first systems biologyoriented studies with Phaeobacter inhibens DSM 17395 (formerly deposited as Phaeobacter gallaeciensis DSM 17395) (12) were published covering the analysis of the proteomic and metabolic response to rich medium and amino acid-containing medium (13,14). In comparison with D. shibae DFL12 T , P. inhibens DSM 17395 is able to degrade a wide range of carbon sources.…”