Molecular and Phenotypic Characterization of Potentially New
            <i>Shigella dysenteriae</i>
            Serotype

Coimbra, Roney Santos; Lenormand, Pascal; Grimont, Francine; Bouvet, Philippe; Matsushita, Shigeru; Grimont, Patrick A. D.

doi:10.1128/jcm.39.2.618-621.2001

Cited by 23 publications

(22 citation statements)

References 31 publications

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“…makes serological identification a crucial step in the diagnosis of Shigella infection (6). According to recent reports, S. flexneri has eight serotypes, of which serotypes 1 to 5 are further classified into 12 subserotypes.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Serologically Atypical Strains ofShigella flexneriType 4 Isolated in Dhaka, Bangladesh

et al. 2002

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Twenty-one atypical Shigella flexneri type 4 strains isolated from patients attending the Dhaka treatment center of the International Centre for Diarrhoeal Disease Research, Bangladesh, were extensively characterized and compared with S. flexneri serotypes 4a and 4b. The atypical strains agglutinated only with the type antigen factor 4 and did not agglutinate with any group factors, thereby excluding their characterization into serotype 4a or 4b. Of the 21 strains, 85.7% did not ferment mannitol but were able to ferment most of the sugars, whereas the remaining 14.3% strains fermented mannitol but were unable to ferment most of the sugars. Most of the strains were resistant to ampicillin, tetracycline, and trimethoprim-sulfomethoxazole. All of the strains harbored the 140-MDa plasmid, had the ipaH gene, had the sen gene (encoding Shigella enterotoxin 2), had the ability to bind Congo red, and were positive for keratoconjunctivitis in the guinea pig eye, attesting their invasive properties. All of the strains contained a middle-range plasmid (35 to 62 MDa) as well as a number of stable small plasmids, yielding mainly two plasmid profiles which were different from those of 4a and 4b strains. Conjugation and curing experiments suggested that the middle-range plasmids harbored a self-transferable multiple antibiotic resistance marker. Pulsed-field gel electrophoresis analysis of all of the tested strains yielded two types with numerous subtypes, whereas ribotyping yielded only two types which were completely different from those of types 4a and 4b. This study concluded that two different clones of atypical S. flexneri type 4 exist and strongly suggests that these are new subserotypes of S. flexneri that await further serological classification.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Serologically Atypical Strains ofShigella flexneriType 4 Isolated in Dhaka, Bangladesh

et al. 2002

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“…), il n'existe pas de milieu d'enrichissement spécifique. L'identification des colonies se fonde sur des réactions biochimiques (fermentation des sucres pour le diagnostic de groupe) et d'agglutination sur lame (diagnostic de sérogroupe) amenant au sérotypage de la souche qui doit idéalement être adressée, en France, au Centre national de référence pour surveillance épidémiologique et identification biotypique voire lysotypique par biologie moléculaire [52].…”

Section: Diagnostic Au Laboratoireunclassified

Shigellose ou dysenterie bacillaire

2007

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“…In view of this fact, these organisms have been placed in the same genomic group (Coimbra et al, 2001). …”

Section: Ribotyping In Shigella Isolatesmentioning

confidence: 99%

Ribotyping as a Molecular Biological Technique for Studying Diversity in <i>Shigella</i> Isolates - A Review

Pal¹

2014

ILNS

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Diarrhoea in developing countries is caused by an increasingly long list of bacterial, viral, and parasitic pathogens with rotavirus, Enterotoxigenic Escherichia coli, Campylobacter, Shigella, and Salmonella heading the list. Using methods to detect most of the known enteropathogens, one or more enteropathogen(s) is isolated in two-thirds of diarrhoeal illnesses in the developing world. Deoxyribonucleic acid probes have proved very useful in detecting pathogens such as enterotoxigenic (ETEC), enteroinvasive (EIEC), enteropathogenic E. coli (EPEC), and Shigella but have not yet proved to be particularly rapid or less expensive. Molecular biology has proved useful in epidemiological studies as a means of strain identification and detection of genome diversity. Since the introduction of ribonucleic acid gene restriction patterns as taxonomic tools in 1986, ribotyping has become an established method for systematics, epidemiological, ecological population and genome diversity studies of microorganisms including Shigella. The technological development culminated in the automation of ribotyping which allowed for high-throughput applications. PCR ribotyping has proved being a highly discriminatory, flexible, robust and cost-efficient routine technique which makes inter-laboratory comparison and build of ribotype databases possible, too. The aim of the present review is to determine the present status of ribotyping technique in detecting the diversity in Shigella isolates.

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Molecular and Phenotypic Characterization of Potentially New Shigella dysenteriae Serotype

Cited by 23 publications

References 31 publications

Phenotypic and Genotypic Characterization of Serologically Atypical Strains ofShigella flexneriType 4 Isolated in Dhaka, Bangladesh

Phenotypic and Genotypic Characterization of Serologically Atypical Strains ofShigella flexneriType 4 Isolated in Dhaka, Bangladesh

Shigellose ou dysenterie bacillaire

Ribotyping as a Molecular Biological Technique for Studying Diversity in <i>Shigella</i> Isolates - A Review

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