Molecular and phenotypic characterization of Xanthomonas campestris pv. campestris causing black rot in Brassica crops in Brazil

Batista, Josefa Neiane Goulart; Ferreira, Marisa Álvares da Silva Velloso; Quezado-Duval, A. M.

doi:10.1007/s40858-021-00456-y

Cited by 5 publications

(1 citation statement)

References 35 publications

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“…A previous study has suggested incidental infections of tomato plants can occur with Xanthomonas campestris pv. campestris (Batista et al, 2021), although no evidence exists that this pathovar can be found on tomato seed. In the case of a positive reaction with only the XopD test, other tests should be used to confirm the identity of the isolate.…”

Section: Discussionmentioning

confidence: 99%

Development and validation of real‐time PCR tests for specific identification of Xanthomonas species causing bacterial spot disease on tomato (Solanum lycopersicum) and pepper (Capsicum annuum)

et al. 2023

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Bacterial spot is a significant disease of tomato and pepper crops caused by members of four Xanthomonas pathogens from three species, namely Xanthomonas euvesicatoria pv. euvesicatoria, Xanthomonas euvesicatoria pv. perforans, Xanthomonas vesicatoria and Xanthomonas hortorum pv. gardneri. These Gram‐negative bacterial plant pathogens are recommended for regulation as quarantine pests by the European and Mediterranean Plant Protection Organization and are currently regulated on tomato and pepper seeds in many regions of the world. Tomato and pepper seeds are a transmission pathway for all three species. Real‐time PCR TaqMan tests were developed and validated for specific identification of isolates from tomato and pepper seeds of these pathogens. These tests are designed to be used in seed health tests after isolation on semiselective media to reduce the number of isolates that need testing in follow‐up pathogenicity tests. A second set of real‐time TaqMan PCR tests was subsequently developed targeting two markers for each taxonomic group of strains. The analytical specificity of all developed tests was evaluated with in vitro and in silico analyses. Diagnostic specificity and sensitivity were also validated on collections of isolates in several laboratories.

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Section: Discussionmentioning

confidence: 99%

Development and validation of real‐time PCR tests for specific identification of Xanthomonas species causing bacterial spot disease on tomato (Solanum lycopersicum) and pepper (Capsicum annuum)

et al. 2023

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Pathogenicity assays on Arabidopsis thaliana infected with Xanthomonas campestris pv. campestris seize aggressiveness variation at the race and isolate levels

et al. 2022

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Field Evaluations of Plant Defense Activators and Sulfur as Alternatives to Copper Bactericides for the Management of Cabbage Black Rot in Florida

Ramirez,

Dufault,

Paret

et al. 2024

Plant Health Progress

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The bacterium Xanthomonas campestris pv. campestris causes black rot, one of the most important diseases affecting brassica production worldwide. Typically, the foliar application of copper-based bactericides is used to manage the disease. However, reports of copper tolerance among xanthomonads, expanded production of diverse brassica crops, and inherent climate challenges have motivated the brassica industry in Florida to evaluate alternatives to copper for improved black rot management. Across nine cabbage trials conducted in 2021 and 2022 in Florida, twelve alternatives were evaluated individually or as tank mixtures within a program. Each trial included a copper hydroxide standard (Kocide 3000) and a nontreated control. Based on the area under disease progress curve (AUDPC), solo applications of the plant defense activators Actigard (acibenzolar-S-methyl), Howler (Pseudomonas chlororaphis), Regalia (Reynoutria sachalinensis extract), ReyZox (Reynoutria sachalinensis extract + azoxystrobin), Theia (Bacillus subtilis), and Vacciplant (laminarin) reduced disease severity compared to the nontreated control and performed equivalent to or better than the Kocide 3000 standard across trials. Howler, Regalia, and Kocide 3000 increased cabbage yields compared to the non-treated control in at least one trial, while Actigard failed to improve yield in any trial. Applications of Microthiol (sulfur), either alone or as a tank mixture, failed to reduce disease severity or improve yield. Regardless of the program, maximum disease control at the final rating was limited to 8% compared to the nontreated control, indicating a need to improve integrated approaches for black rot management.

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Molecular and phenotypic characterization of Xanthomonas campestris pv. campestris causing black rot in Brassica crops in Brazil

Cited by 5 publications

References 35 publications

Development and validation of real‐time PCR tests for specific identification of Xanthomonas species causing bacterial spot disease on tomato (Solanum lycopersicum) and pepper (Capsicum annuum)

Development and validation of real‐time PCR tests for specific identification of Xanthomonas species causing bacterial spot disease on tomato (Solanum lycopersicum) and pepper (Capsicum annuum)

Pathogenicity assays on Arabidopsis thaliana infected with Xanthomonas campestris pv. campestris seize aggressiveness variation at the race and isolate levels

Field Evaluations of Plant Defense Activators and Sulfur as Alternatives to Copper Bactericides for the Management of Cabbage Black Rot in Florida

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