Molecular and phenotypic characterization of methicillin-resistant Staphylococcus aureus isolates causing bacteremia at a major hospital in southern Mississippi

Samanta, Dhritiman; Batte, Justin L.; Brown, S; Crosby, Angela G.; Marcos, Luis A.; Elasri, Mohamed O.

doi:10.1016/j.ajic.2015.01.023

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…Similarly, a 15-year study in Taiwan concluded that CA-MRSA strains have replaced HA-MRSA in neonatal units [5]. Also, a predominance of CA-MRSA in the skin and soft tissue infections (SSTIs) and bacteremia isolates has been reported in Australia, China, the USA, and North Africa among other regions [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Multidrug-Resistant Staphylococcus aureus Isolates in a Tertiary Care Hospital, Kingdom of Bahrain

Al-Saleh¹,

Shahid²,

Farid³

et al. 2023

Cureus

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a ubiquitous pathogen associated with a wide spectrum of human infections. In recent decades, MRSA infections have been increasingly reported in individuals without established risk factors, infecting immunocompetent members of the community. This emergence is attributed to the production of various virulence factors, notably Panton-Valentine leukocidin (PVL).Objective: The aim of this study was to better understand the prevalence, antibiotic resistance profiles, and molecular characteristics of S. aureus and MRSA in a tertiary care hospital in the Kingdom of Bahrain.Materials and methods: This cross-sectional study was carried out in a tertiary hospital for a one-year period, from December 2020 to December 2021. A total of 161 consecutive S. aureus isolates were collected. Antibiotic susceptibility was tested using BD Phoenix™ automated identification and susceptibility testing system. Molecular analysis was conducted via conventional PCR and conventional multiplex PCR for SCCmec typing.Results: In this study, 161 S. aureus isolates were investigated, 60% (n=97) were characterized as MRSA, of which, 12% (n=12) were healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) while 88% (n=85) were community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA). No statistically significant difference (P>0.05) in antibiotic resistance trends between HA-MRSA and CA-MRSA was detected. Multidrug resistance (MDR) amounted to 19% (n=30) of all S. aureus isolates, 14% (n=9) of methicillin-susceptible Staphylococcus aureus (MSSA) isolates, and 22% (n=21) of MRSA isolates. SCC mec typing demonstrated a high prevalence of type IV (61%, n=59), followed by type V (32%, n=31), then type II (4%, n=4), and type III (3%, n=3). The PVL prevalence was 39% (n=25) in MSSA and 62% (n=60) in MRSA, 33% (n=4) in HA-MRSA, and 66% (n=56) in CA-MRSA.Conclusion: This study demonstrated the emergence of PVL-producing CA-MRSA in a tertiary care hospital, as well as the detection of PVL-producing MDR strains. This development prompts serious measures to be taken in order to sustain a healthy clinical environment.

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Section: Introductionmentioning

confidence: 99%

Multidrug-Resistant Staphylococcus aureus Isolates in a Tertiary Care Hospital, Kingdom of Bahrain

Al-Saleh¹,

Shahid²,

Farid³

et al. 2023

Cureus

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“…Staphylococcus aureus (S. aureus) is a common pathogen and can cause different kinds of infection in both hospitals and communities (Knox et al, 2015;Samanta et al, 2015). Several parts of human body can carry S. aureus and the nasal cavity is the main part.…”

Section: Introductionmentioning

confidence: 99%

Prevalence, Influencing Factors, Antibiotic Resistance, Toxin and Molecular Characteristics of Staphylococcus aureus and MRSA Nasal Carriage among Diabetic Population in the United States, 2001–2004

LIN

Yang

Bai

et al. 2017

Polish Journal of Microbiology

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Diabetic population were reported more likely to suffer carriage and infection with Staphylococcus aureus (S. aureus) and methicillin-resistant Staphylococcus aureus (MRSA) than non-diabetic population. We aim to elucidate the prevalence and characteristics of S. aureus and MRSA nasal carriage among diabetic population in the United States National Health and Nutrition Examination Survey, 2001-2004. Univariate analyses were conducted using Chi-square test, Fisher's exact probability test or student t test, as appropriate. Multivariate analy¬sis using logistic regression was conducted to assess the association between influencing factors and S. aureus and MRSA nasal carriage. 1010 diabetic participants were included in the study. The prevalence of S. aureus and MRSA nasal carriage were 28.32% and 1.09%, respec¬tively. After the logistic regression, ever had a painful sensation or tingling in hands or feet past three months (Odds Ratio [OR] = 0.359, 95% Confidence Interval [CI], 0.146-0.882) was significant among S. aureus nasal carriage and gender (OR = 3.410, 95% CI, 1.091-10.653) was significant among MRSA nasal carriage. The proportions of staphylococcal enterotoxin (SE) A, SEB, SEC, SED, Toxic-shock syn¬drome toxin-1, and Panton Valentine Leukocidin toxin among S. aureus strains were 18.75%, 3.13%, 12.50%, 15.63%, 28.13%, and 9.38%, respectively. 63.63% of MRSA strains were community-acquired, 27.27% were hospital-acquired, and 9.09% were non-typeable. Diabetic patients might be more likely to carry S. aureus and MRSA in the United States. Improving hand hygiene compliance, reducing antibiotic overuse, screening for carriers, and decolonization are recommended to reduce the spread of S. aureus and MRSA, especially in community.

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Molecular and phenotypic characterization of methicillin-resistant Staphylococcus aureus isolates causing bacteremia at a major hospital in southern Mississippi

Cited by 2 publications

References 20 publications

Multidrug-Resistant Staphylococcus aureus Isolates in a Tertiary Care Hospital, Kingdom of Bahrain

Multidrug-Resistant Staphylococcus aureus Isolates in a Tertiary Care Hospital, Kingdom of Bahrain

Prevalence, Influencing Factors, Antibiotic Resistance, Toxin and Molecular Characteristics of Staphylococcus aureus and MRSA Nasal Carriage among Diabetic Population in the United States, 2001–2004

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