Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas.

Dourdin, Thomas Sol; Rivière, Guillaume; Cormier, Alexandre; Poi, Carole Di; Guyomard, Killian; Rabiller, Manuella; Akcha, Farida; Sadialiou, Thierno Bah; Monier, Pauline Le; Sussarellu, Rossana

doi:10.1016/j.envpol.2023.121472

Cited by 5 publications

(24 citation statements)

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“…Another hypothesis of this study was the role of DNA methylation in mediating environmentally induced effects, which is now commonly admitted. , First, in gastrula embryos, DMRs were significantly enriched in intergenic regions (Figure SI9), as already observed in F0, although dedicated studies are needed to clarify functional consequences of the methylation of noncoding regions and transposons. Second, it is remarkable that pesticide-induced differential methylation followed opposite trends in the F0 (hypomethylation) and F1 (hypermethylation) generations.…”

Section: Discussionmentioning

confidence: 74%

“…The pesticide mixture used was the same as the one used in the F0 generation . Briefly, the pesticides of the mixture were chosen based on their reported presence in the main French oyster farming areas during the reproduction period of the oyster.…”

Section: Methodsmentioning

confidence: 99%

“…The embryotoxicity test was conducted in triplicate according to the previously described protocol, following the standardized embryo–larval bioassay ISO 17244:2015 based on the counting of larval abnormalities. During larval rearing, larvae were sampled in each flow-through tank every 2–3 days and stored in a 0.1% seawater–formaldehyde solution until image analysis as described in ref . The metamorphosis bioassay was conducted on competent eye-spotted pediveliger larvae retrieved on a 150 μm sieve (14 dpf, 12 days post exposure) in 12-well microplates using epinephrine (Sigma-Aldrich) at a 10 –4 M final concentration as described in ref .…”

Section: Methodsmentioning

confidence: 99%

“…During larval rearing, larvae were sampled in each flow-through tank every 2–3 days and stored in a 0.1% seawater–formaldehyde solution until image analysis as described in ref . The metamorphosis bioassay was conducted on competent eye-spotted pediveliger larvae retrieved on a 150 μm sieve (14 dpf, 12 days post exposure) in 12-well microplates using epinephrine (Sigma-Aldrich) at a 10 –4 M final concentration as described in ref . Spat growth and on-field survival monitoring were conducted following the protocols used for the F0 generation …”

Section: Methodsmentioning

confidence: 99%

“…The metamorphosis bioassay was conducted on competent eye-spotted pediveliger larvae retrieved on a 150 μm sieve (14 dpf, 12 days post exposure) in 12-well microplates using epinephrine (Sigma-Aldrich) at a 10 –4 M final concentration as described in ref . Spat growth and on-field survival monitoring were conducted following the protocols used for the F0 generation …”

Section: Methodsmentioning

confidence: 99%

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Ancestors’ Gift: Parental Early Exposure to the Environmentally Realistic Pesticide Mixture Drives Offspring Phenotype in a Larger Extent Than Direct Exposure in the Pacific Oyster, Crassostrea gigas

Sol Dourdin,

Guyomard,

Rabiller

et al. 2024

Environ. Sci. Technol.

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Marine organisms are threatened by the presence of pesticides in coastal waters. Among them, the Pacific oyster is one of the most studied invertebrates in marine ecotoxicology where numerous studies highlighted the multiscale impacts of pesticides. In the past few years, a growing body of literature has reported the epigenetic outcomes of xenobiotics. Because DNA methylation is an epigenetic mark implicated in organism development and is meiotically heritable, it raises the question of the multigenerational implications of xenobioticinduced epigenetic alterations. Therefore, we performed a multigenerational exposure to an environmentally relevant mixture of 18 pesticides (nominal sum concentration: 2.85 μg•L −1 ) during embryo−larval stages (0−48 hpf) of a second generation (F1) for which parents where already exposed or not in F0. Gene expression, DNA methylation, and physiological end points were assessed throughout the life cycle of individuals. Overall, the multigenerational effect has a greater influence on the phenotype than the exposure itself. Thus, multigenerational phenotypic effects were observed: individuals descending from exposed parents exhibited lower epinephrine-induced metamorphosis and field survival rates. At the molecular level, RNA-seq and Methyl-seq data analyses performed in gastrula embryos and metamorphosis-competent pediveliger (MCP) larvae revealed a clear F0 treatment-dependent discrimination. Some genes implicated into shell secretion and immunity exhibited F1:F0 treatment interaction patterns (e.g., Calm and Myd88). Those results suggest that low chronic environmental pesticide contamination can alter organisms beyond the individual scale level and have long-term adaptive implications.

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Section: Discussionmentioning

confidence: 74%

Section: Methodsmentioning

confidence: 99%