Molecular and Physiological Analysis of Growth-Limiting Drought Stress in Brachypodium distachyon Leaves

Verelst, Wim; Bertolini, Edoardo; Bodt, Stefanie De; Vandepoele, Klaas; Demeulenaere, Marlies; Pè, Mario Enrico; Inzé, Dirk

doi:10.1093/mp/sss098

Cited by 104 publications

(78 citation statements)

References 46 publications

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“…Drought stress resulted in the lowest DEG number (Fig. 3C), which is in accordance with a previous report (Verelst et al, 2013). In general, a prominent increase in the percentage of DEGs was found under combinations of stresses, where 24-30% of the transcriptome was modulated ( Fig.…”

Section: Integration Between Transcriptional Changes and Physiologicasupporting

confidence: 92%

Unique Physiological and Transcriptional Shifts under Combinations of Salinity, Drought, and Heat

Shaar-Moshe

Blumwald²,

Peleg³

2017

Plant Physiol.

112

113

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Climate-change-driven stresses such as extreme temperatures, water deficit, and ion imbalance are projected to exacerbate and jeopardize global food security. Under field conditions, these stresses usually occur simultaneously and cause damages that exceed single stresses. Here, we investigated the transcriptional patterns and morpho-physiological acclimations of Brachypodium dystachion to single salinity, drought, and heat stresses, as well as their double and triple stress combinations. Hierarchical clustering analysis of morpho-physiological acclimations showed that several traits exhibited a gradually aggravating effect as plants were exposed to combined stresses. On the other hand, other morphological traits were dominated by salinity, while some physiological traits were shaped by heat stress. Response patterns of differentially expressed genes, under single and combined stresses (i.e. common stress genes), were maintained only among 37% of the genes, indicating a limited expression consistency among partially overlapping stresses. A comparison between common stress genes and genes that were uniquely expressed only under combined stresses (i.e. combination unique genes) revealed a significant shift from increased intensity to antagonistic responses, respectively. The different transcriptional signatures imply an alteration in the mode of action under combined stresses and limited ability to predict plant responses as different stresses are combined. Coexpression analysis coupled with enrichment analysis revealed that each gene subset was enriched with different biological processes. Common stress genes were enriched with known stress response pathways, while combination unique-genes were enriched with unique processes and genes with unknown functions that hold the potential to improve stress tolerance and enhance cereal productivity under suboptimal field conditions.

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Section: Integration Between Transcriptional Changes and Physiologicasupporting

confidence: 92%

Unique Physiological and Transcriptional Shifts under Combinations of Salinity, Drought, and Heat

Shaar-Moshe

Blumwald²,

Peleg³

2017

Plant Physiol.

112

113

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“…The decline in the photosynthetic rate under both stresses are frequently attributed to lowered internal CO 2 , inhibition of photosynthetic enzymes (eg Rubisco) and synthesis of ATP (Arasimowicz Lidon, 2012). However, it was also shown that mild water stress decreased biomass production without a significant effect on photosynthesis (Verelst et al, 2012). This demonstrates that plants reduce their growth as an adaptation response to stress rather than as a secondary consequence of resource limitations (Rollins et al, 2013).…”

Section: Transpiration and Photosynthesismentioning

confidence: 94%

Effect of drought and heat stresses on plant growth and yield: a review

Lipiec¹,

Doussan²,

Nosalewicz³

et al. 2013

International Agrophysics

469

225

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A b s t r a c t. Drought and heat stresses are important threat limitations to plant growth and sustainable agriculture worldwide. Our objective is to provide a review of plant responses and adaptations to drought and elevated temperature including roots, shoots, and final yield and management approaches for alleviating adverse effects of the stresses based mostly on recent literature. The sections of the paper deal with plant responses including root growth, transpiration, photosynthesis, water use efficiency, phenotypic flexibility, accumulation of compounds of low molecular mass (eg proline and gibberellins), and expression of some genes and proteins for increasing the tolerance to the abiotic stresses. Soil and crop management practices to alleviate negative effects of drought and heat stresses are also discussed. Investigations involving determination of plant assimilate partitioning, phenotypic plasticity, and identification of most stress-tolerant plant genotypes are essential for understanding the complexity of the responses and for future plant breeding. The adverse effects of drought and heat stress can be mitigated by soil management practices, crop establishment, and foliar application of growth regulators by maintaining an appropriate level of water in the leaves due to osmotic adjustment and stomatal performance.K e y w o r d s: water stress, high temperature, root and shoot growth, tolerance mechanisms, management practices INTRODUCTIONPlants are frequently exposed to drought and heat stresses that reduce crop yield worldwide. The combined effect of both heat and drought on yield of many crops is stronger than the effects of each stress alone (Dreesen et al., 2012;Rollins et al., 2013).Agricultural water deficit arises from both insufficient rainfall and soil water during the growing season to sustain a high crop yield (Sekhon et al., 2010;Vadez et al., 2011;2012;). Projections show an increase in intense rain events and at the same time reduction in the number of rain days that leads to increased risk of drought (Trenberth, 2011;Vadez et al., 2011). Therefore, under rainfed conditions water scarcity is one of the most widespread limitations to crop production.A period of dry weather, injurious to crops, is often defined as 'drought' that is related to changes in soil and meteorological conditions and not with plant and tissue hydration. Drought stress occurs when the humidity of the soil and the relative air humidity are low and the ambient temperature is high.Predisposition of plants to maintain a high potential of water in the tissues under drought is called dehydration avoidance, and tolerance that determines plant predisposition to survive water deficiency is called drought resistance (Blum, 2005;Vadez et al., 2011). Molecular biologists often report the effect of an exotic gene towards 'drought tolerance' and advertise its expected value in breeding (Blum, 2005).Heat stress or heat wave is defined as the rise in temperature beyond a threshold level for a period sufficient to cause permanent ...

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“…Reduced water availability leads to drought stress, which is a major constraint on the physiology, growth, development, and productivity of plants (Boyer, 1970(Boyer, , 1982Lobell and Field, 2007;Roberts and Schlenker, 2009;Skirycz et al, 2010;Lobell et al, 2011;Verelst et al, 2013). Therefore, understanding the mechanisms of drought response in plants is essential for the improvement of plant performance under water-limiting conditions and has been the subject of many investigations over the years Yamaguchi-Shinozaki, 1997, 2007;Chaves et al, 2009;Nakashima et al, 2009;Pinheiro and Chaves, 2011).…”

Section: Introductionmentioning

confidence: 99%

Time-Series Transcriptomics Reveals That AGAMOUS-LIKE22 Affects Primary Metabolism and Developmental Processes in Drought-Stressed Arabidopsis

et al. 2016

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In Arabidopsis thaliana, changes in metabolism and gene expression drive increased drought tolerance and initiate diverse drought avoidance and escape responses. To address regulatory processes that link these responses, we set out to identify genes that govern early responses to drought. To do this, a high-resolution time series transcriptomics data set was produced, coupled with detailed physiological and metabolic analyses of plants subjected to a slow transition from well-watered to drought conditions. A total of 1815 drought-responsive differentially expressed genes were identified. The early changes in gene expression coincided with a drop in carbon assimilation, and only in the late stages with an increase in foliar abscisic acid content. To identify gene regulatory networks (GRNs) mediating the transition between the early and late stages of drought, we used Bayesian network modeling of differentially expressed transcription factor (TF) genes. This approach identified AGAMOUS-LIKE22 (AGL22), as key hub gene in a TF GRN. It has previously been shown that AGL22 is involved in the transition from vegetative state to flowering but here we show that AGL22 expression influences steady state photosynthetic rates and lifetime water use. This suggests that AGL22 uniquely regulates a transcriptional network during drought stress, linking changes in primary metabolism and the initiation of stress responses.

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Molecular and Physiological Analysis of Growth-Limiting Drought Stress in Brachypodium distachyon Leaves

Cited by 104 publications

References 46 publications

Unique Physiological and Transcriptional Shifts under Combinations of Salinity, Drought, and Heat

Unique Physiological and Transcriptional Shifts under Combinations of Salinity, Drought, and Heat

Effect of drought and heat stresses on plant growth and yield: a review

Time-Series Transcriptomics Reveals That AGAMOUS-LIKE22 Affects Primary Metabolism and Developmental Processes in Drought-Stressed Arabidopsis

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