Molecular and Serologic Characterization of a Group a Bovine Rotavirus with a Short Genome Pattern

Parwani, Anil V.; Muñoz, Macarena; Tsunemitsu, Hiroshi; Lucchelli, Alejandro; Saif, Linda J.

doi:10.1177/104063879500700216

Cited by 3 publications

(8 citation statements)

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“…On the other hand, G6 is regarded as an uncommon genotype in human RV and has been rarely detected in humans [7][8][9][10], although it is prevalent in cattle combined with P [5], P [1], and P [11] [5,12,[25][26][27][28][29]. To date, several G6 human RV strains have been examined for their origin based on the analysis of whole genomic sequences [4,[30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Detection and full genomic analysis of G6P[9] human rotavirus in Japan

et al. 2011

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A rare genotype G6P[9] was identified in two human group A rotavirus strains designated as KF14 and KF17, that were detected in stool specimens from children with diarrhea in Japan. VP7 gene sequences of these two strains were identical and genetically closely related to G6 human rotavirus strains reported in European countries and the United States. To our knowledge, this is the first report of detection of a G6 human rotavirus in Japan. For further genetic analysis to elucidate the origin of the G6 rotavirus, nearly full-length sequences of all 11 RNA segments were determined for the KF17 strain. The complete genomic constellation of KF17 was determined as G6-P[9]-I2-R2-C2-M2-A3-N2-T3-E3-H3, a novel genotype constellation for human rotavirus. Phylogenetic analysis indicated that VP6, VP1-3, and NSP2 genes of KF17 clustered with bovine-like G6 human strains and some animal strains into sub-lineages distinct from those of common DS-1-like G2 human rotaviruses. On the other hand, KF17 genes encoding VP4, NSP1, and NSP3-5 showed high sequence identities to the human G3P[9] strain AU-1, and clustered with AU-1 and some feline strains within the same lineage. These findings suggested that the G6P[9] human rotavirus detected in Japan may have occurred through reassortment among uncommon bovine-like human rotaviruses and human/feline AU-1-like rotaviruses.

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Section: Discussionmentioning

confidence: 99%

Detection and full genomic analysis of G6P[9] human rotavirus in Japan

et al. 2011

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“…Because each strain produces a unique genome profile, the electropherotyping of rotavirus field strains by PAGE is a useful tool not only for diagnosis but also for obtaining important epidemiological in-formation about the origin of field strains and variation and diversity among these strains (2,11,15,21,23). In addition to the usual ''long''-genome electropherotypes, short-genome electropherotypes, in which the smallest two segments appear to migrate more slowly than usual, have been described for group A rotaviruses (1,7,8,10,20,23). These short genome electropherotypes are relatively uncommon, have been restricted to group A, and have been described only in humans, rabbits, and calves (19).…”

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confidence: 99%

“…The sources of the reference rotaviruses used in this study have been described previously (6,7,11,15,24). The reference group A and C viruses were grown in rhesus monkey kidney (MA-104) cells in roller bottles and titrated by a plaque assay as previously described (11,12,15,18,24).…”

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confidence: 99%

“…Gnotobiotic calf experiments were conducted to passage field group B rotaviruses, examine their pathogenicity, and generate large quantities of stock virus (since group B rotaviruses do not replicate in cell culture). Bovine group B rotaviruses were passaged in three newborn gnotobiotic calves, derived, fed, inoculated, and maintained as previously described (7,16,17). Calf A was oronasally inoculated with a fecal filtrate of live virulent bovine group B strain BB-RVLV (previously passaged in a conventional colostrum-deprived calf), and calves B and C were oronasally inoculated with a fecal filtrate of the original cow specimen of bovine group B rotavirus KD strain.…”

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confidence: 99%

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Identification of group B rotaviruses with short genome electropherotypes from adult cows with diarrhea

1996

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Two field strains (BB-RVLV and KD) of group B rotaviruses from adult dairy cows with diarrhea displayed short genome electropherotypes. Gnotobiotic calves inoculated with fecal filtrates of each group B rotavirus developed diarrhea, and only group B rotaviruses or antigens were detected in the feces by immunoelectron microscopy and in intestinal epithelial cells by immunofluorescent staining, respectively. The feces or intestinal contents of the cows and inoculated calves were negative for group A and C rotaviruses by enzyme-linked immunosorbent assay, immunoelectron microscopy, or cell culture immunofluorescence assays. Comparison of the genome electropherotypes of the calf-passaged BB-RVLV and KD strains with the original samples and reference bovine group A, B, and C rotaviruses revealed conservation of their short-genome electropherotypes and double-stranded RNA migration patterns characteristic of group B rotaviruses. To our knowledge, our previous study (L. J. Saif, K. V. Brock, D. R. Redman, and E. M. Kohler, Vet. Rec. 128:447-449, 1991) and this report are the first description of bovine group B rotaviruses (in a mixed infection with bovine coronavirus or singly in fecal contents) in adult cows with diarrhea and this is the first report of short-genome electropherotypes among group B rotaviruses.

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“…In general, the migration pattem of group A BRV is long (Theil, 1990). However, there have been reports of short and super-short migration patterns among these viruses (Paul et al, 1988;Theil et al, 1988;Allen et al, 1989;Parwani et al, 1995;Chinasagaram et al, 1995). The supershort migration pattem in the VMRI strain is due to the rearrangement in the 11th genomic segment (Paul et al, 1988;.…”

Section: Genome and Geneticsmentioning

confidence: 99%

Characterization of the P and G types and the VP4 and VP7 gene divergence among group A bovine rotaviruses

Mummidi

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Molecular and Serologic Characterization of a Group a Bovine Rotavirus with a Short Genome Pattern

Cited by 3 publications

References 34 publications

Detection and full genomic analysis of G6P[9] human rotavirus in Japan

Detection and full genomic analysis of G6P[9] human rotavirus in Japan

Identification of group B rotaviruses with short genome electropherotypes from adult cows with diarrhea

Characterization of the P and G types and the VP4 and VP7 gene divergence among group A bovine rotaviruses

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