Molecular and serologic survey of Orientia tsutsugamushi infection among field rodents in southern Cholla Province, Korea.

Song, Hyun Jae; Seong, Seung Yong; Huh, Myung Soo; Park, Sae Gwang; Jang, Won Jong; Kee, Sun Ho; Kim, Kai Hwan; Kim, Sun Chun; Choi, Myung Sik; Kim, Ik Sang; Chang, Woo Hyun

doi:10.4269/ajtmh.1998.58.513

Cited by 19 publications

(12 citation statements)

References 9 publications

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“…For example, Jackson et al (8) isolated O. tsutsugamushi from 12 (13.2%) among 91 wild rodents in Korea. Using PCR, O. tsutsugamushi DNA was detected in 12 (9.5%) among 127 wild rodents (9). In studies with experimentally infected mice, persistence of O. tsutsugamushi was more clearly demonstrated (10-13).…”

Section: Discussionmentioning

confidence: 99%

Persistence ofOrientia tsutsugamushiin Humans

et al. 2012

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We investigated the persistence of viable Orientia tsutsugamushi in patients who had recovered from scrub typhus. Blood specimens were available from six patients with scrub typhus who were at 1 to 18 months after the onset of the illness. The EDTA-treated blood specimens were inoculated into ECV304 cells, and cultures were maintained for 7 months. Sequencing of the 56-kDa type-specific antigen gene of O. tsutsugamushi was performed to ascertain the homology of isolates. O. tsutsugamushi was isolated from all six patients, and nucleotide sequences of isolates serially collected from each patient were identical in all five patients in whom nucleotide sequences were compared. One patient relapsed 2 days after completion of antibiotic therapy; two patients complained of weakness for 1 to 2.5 months after the illness; one patient underwent coronary angioplasty 6 months later; and one patient suffered from a transient ischemic attack 8 months later. This finding suggests that O. tsutsugamushi causes chronic latent infection, which may be associated with certain clinical illnesses, preceded by scrub typhus. Antibiotic therapy abates the symptoms of scrub typhus, but does not eradicate O. tsutsugamushi from the human body.

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Section: Discussionmentioning

confidence: 99%

Persistence ofOrientia tsutsugamushiin Humans

et al. 2012

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“…Scrub typhus is one of the most prevalent febrile illnesses in Korea (19). R. typhi and R. prowazekii have also been isolated in Korea, although there has been no recent documentation of this (1).…”

Section: Discussionmentioning

confidence: 99%

Differentiation of Rickettsiae by groEL Gene Analysis

Lee¹,

Park²,

Jang³

et al. 2003

J Clin Microbiol

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The nucleotide sequences (534 to 546 bp) of the groEL gene, which encodes the 60-kDa heat shock protein GroEL, from 15 rickettsial strains were determined and compared. In the phylogenetic tree created by the unweighted pair group method with arithmetic averages and the neighbor-joining method, rickettsial strains could be distinguished from Ehrlichia strains. Five spotted fever group strains, four typhus group strains, and six scrub typhus group (STG) strains were differentiated as distinct entities. Unlike gltA and ompA gene analyses, differentiation between members of the genus Rickettsia and the STG rickettsiae by groEL gene analysis was possible. In comparison with 16S rRNA gene analysis, the groEL gene has a higher degree of divergence among the rickettsiae. We therefore successfully developed rapid differentiation methods, PCRrestriction fragment length polymorphism analysis and a species-specific PCR, based on the groEL gene sequences. Four Korean isolates were identified by these methods and groEL gene analysis. The results suggest that the groEL gene is useful for the identification and characterization of rickettsiae.

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“…Although they reported similar specificity, Sonthayanon et al [173] noted a sensitivity of only 45% for real-time PCR with 16S rDNA-derived primers in analysis of blood from 183 patients with serologically confirmed disease in Thailand. PCR assays also allow monitoring of levels of infection in arthropod vectors, mammalian hosts (such as rodents), and nonhuman primates and the comparison of active infection with previous infection (assessed by testing for the presence of antibodies) without the necessity of culturing O. tsutsugamushi [72,159,174,175].…”

Section: Molecular Characterization Of Antigen Genes In Orientia Speciesmentioning

confidence: 99%

Scrub Typhus: The Geographic Distribution of Phenotypic and Genotypic Variants ofOrientia tsutsugamushi

et al. 2009

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Orientia tsutsugamushi is the etiological agent of scrub typhus, an acute, mite-borne, febrile illness that occurs in the Asia-Pacific region. Historically, strain characterization used serological analysis and revealed dramatic antigenic diversity. Eyeing a recommendation of potential vaccine candidates for broad protection, we review geographic diversity and serological and DNA prevalences. DNA analysis together with immunological analysis suggest that the prototype Karp strain and closely related strains are the most common throughout the region of endemicity. According to serological analysis, approximately 50% of isolates are seroreactive to Karp antisera, and approximately one-quarter of isolates are seroreactive to antisera against the prototype Gilliam strain. Molecular methods reveal greater diversity. By molecular methods, strains phylogenetically similar to Karp make up approximately 40% of all genotyped isolates, followed by the JG genotype group (Japan strains serotypically similar to the Gilliam strain but genetically non-Gilliam; 18% of all genotyped isolates). Three other genotype groups (Kato-related, Kawasaki-like, and TA763-like) each represent approximately 10% of genotyped isolates. Strains genetically similar to the Gilliam strain make up only 5% of isolates. Strains from these groups should be included in any potential vaccine.

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Molecular and serologic survey of Orientia tsutsugamushi infection among field rodents in southern Cholla Province, Korea.

Cited by 19 publications

References 9 publications

Persistence ofOrientia tsutsugamushiin Humans

Persistence ofOrientia tsutsugamushiin Humans

Differentiation of Rickettsiae by groEL Gene Analysis

Scrub Typhus: The Geographic Distribution of Phenotypic and Genotypic Variants ofOrientia tsutsugamushi

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