Molecular and serological prevalence of Anaplasma marginale in water buffaloes in northern Brazil

Silva, Jenevaldo Barbosa da; Vinhote, Wagner M.S.; Oliveira, Carlos Magno Chaves; André, Marcos Rogério; Machado, Rosangela Zacarias; Fonseca, Adivaldo Henrique da; Barbosa, José Diomedes

doi:10.1016/j.ttbdis.2013.09.007

Cited by 32 publications

(13 citation statements)

References 19 publications

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“…This finding was however not in agreement with the work of Alfredo et al (2005), where the authors reported a high seroprevalence 63% of bovine anaplasmosis among calves in Tete province of Mozambique. However, the authors also corroborated the findings of Da Silva et al (2014) indicating that higher seroprevalence of bovine anaplasmosisis associated with geographical location as well as the sex of animals. It is important to note that, despite the difference in the number of samples used, the serological test applied in both studies also differs.…”

Section: Seroprevalence Of Bovine Anaplasmosissupporting

confidence: 79%

Seroprevalence of Anaplasmosis in Dairy Cattle from Peninsular Malaysia

Bitrus¹,

Abba²,

Jesse³

et al. 2018

Adv. Anim. Vet. Sci.

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Section: Seroprevalence Of Bovine Anaplasmosissupporting

confidence: 79%

Seroprevalence of Anaplasmosis in Dairy Cattle from Peninsular Malaysia

Bitrus¹,

Abba²,

Jesse³

et al. 2018

Adv. Anim. Vet. Sci.

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“…In the comparative analysis of the snPCR result for msp5 gene among splenectomized animals, cattle presented a significantly higher average frequency of infection than buffaloes. Other studies also reported that buffaloes had lower values (Silva et al 2014a(Silva et al , 2014b compared to the observed in cattle (Bacanelli et al 2014). A splenectomized buffalo was positive only 60 days after inoculation.…”

Section: Discussionmentioning

confidence: 80%

“…Despite the confirmed presence of this rickettsia in buffalo herds (Silva et al 2014a(Silva et al , 2014b(Silva et al , 2014c(Silva et al , 2015, there is little knowledge about the development of anaplasmosis in this species. Furthermore, there is no research that simultaneously evaluates the pathogenicity of A. marginale in cattle and buffaloes and compares the effects of the disease between the two species.…”

Section: Introductionmentioning

confidence: 99%

Experimental infection by Anaplasma marginale in buffaloes and cattle: clinical, hematological, molecular and pathological aspects

et al. 2019

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The study aimed to evaluate and compare the clinical, laboratory and pathological aspects of buffalo and bovine experimentally infected with AmRio 2 strain of Anaplasma marginale. Four Murrah buffaloes and four crossbred cattle were used in the experiment, which two animals of each species were splenectomized. Strain AmRio 2 of A. marginale was inoculated in all experimental animals. Clinical exams, Packed Cell Volume (PCV), blood counts, blood smears, rickettsemia, necropsy and histopathology were performed in all cases. Semi-Nested-PCR (snPCR) for the msp5 and snPCR for the msp1α target gene for identification of A. marginale in blood samples from animals was done. From positive samples for msp1α snPCR, samples were analyzed for the amino acid sequences of this gene. Two splenectomized cattle presented apathy, pale mucous membranes, jaundice, hyperthermia, and severe anemia. The remaining experimental animals did not show clinical signs. The rickettsemia in all animals was less than 1%. The mean PCV of the splenectomized cattle was below 20% at two-time points after infection. On the blood count, the main changes were observed in splenectomized calves and were characterized by a decrease in red blood cells, hemoglobin, PCV and platelets (p <0.05). All animals presented leukocyte elevation by increased lymphocytes, however, with no significant difference. The average prepatent period was two days in all the animals. The average incubation period in cattle that became ill was 25.5 days, and death occurred, on average, 63 days after inoculation of the strain. The necropsy findings were characterized by pale carcass, ascites, enlarged liver, distended gallbladder, and thick bile. Histopathological findings included infiltration of macrophages and lymphocytes in various organs, hepatic sinusoidal dilatation, and necrosis of the large intestine. In snPCR for the msp5 gene, 100% of the animals were positive in at least one evaluation. And in the snPCR for the infection of the msp1α target gene was also found in all animals in at least one sample evaluated. However, sequencing revealed only five animals, including the bovine which died, with a similarity of the amino acid sequences with AmRio 2 strain of A. marginale. It is concluded that the splenectomized cattle died due to anaplasmosis caused by the inoculated strain and the buffalo were more resistant compared to cattle. Buffaloes can be an alternative to cattle rearing in areas with a high occurrence of clinical cases of anaplasmosis.

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“…In fact, numerous studies reported that vegetation cover, grazing system and livestock production system are also involved in the transmission of tick‐borne diseases (Torina et al., ,b; Magona et al., ; Liu et al., ). Other studies also mentioned inter‐regional variations in prevalence of anaplasmosis infection (Sahibi et al., ,b; Torina et al., ,b; Ait Hamou et al., ; Ashraf et al., ; Atif et al., ; Barbosa da Silva et al., ).…”

Section: Discussionmentioning

confidence: 98%

High Prevalence ofAnaplasmaspp. in Small Ruminants in Morocco

Lbacha

Alali

Zouagui

et al. 2015

Transbound Emerg Dis

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The prevalence of infection by Anaplasma spp. (including Anaplasma phagocytophilum) was determined using blood smear microscopy and PCR through screening of small ruminant blood samples collected from seven regions of Morocco. Co-infections of Anaplasma spp., Babesia spp, Theileria spp. and Mycoplasma spp. were investigated and risk factors for Anaplasma spp. infection assessed. A total of 422 small ruminant blood samples were randomly collected from 70 flocks. Individual animal (breed, age, tick burden and previous treatment) and flock data (GPS coordinate of farm, size of flock and livestock production system) were collected. Upon examination of blood smears, 375 blood samples (88.9%) were found to contain Anaplasma-like erythrocytic inclusion bodies. Upon screening with a large spectrum PCR targeting the Anaplasma 16S rRNA region, 303 (71%) samples were found to be positive. All 303 samples screened with the A. phagocytophilum-specific PCR, which targets the msp2 region, were found to be negative. Differences in prevalence were found to be statistically significant with regard to region, altitude, flock size, livestock production system, grazing system, presence of clinical cases and application of tick and tick-borne diseases prophylactic measures. Kappa analysis revealed a poor concordance between microscopy and PCR (k = 0.14). Agreement with PCR is improved by considering microscopy and packed cell volume (PCV) in parallel. The prevalence of double infections was found to be 1.7, 2.5 and 24% for Anaplasma-Babesia, Anaplasma-Mycoplasma and Anaplasma-Theileria, respectively. Co-infection with three or more haemoparasites was found in 1.6% of animals examined. In conclusion, we demonstrate the high burden of anaplasmosis in small ruminants in Morocco and the high prevalence of co-infections of tick-borne diseases. There is an urgent need to improve the control of this neglected group of diseases.

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Molecular and serological prevalence of Anaplasma marginale in water buffaloes in northern Brazil

Cited by 32 publications

References 19 publications

Seroprevalence of Anaplasmosis in Dairy Cattle from Peninsular Malaysia

Seroprevalence of Anaplasmosis in Dairy Cattle from Peninsular Malaysia

Experimental infection by Anaplasma marginale in buffaloes and cattle: clinical, hematological, molecular and pathological aspects

High Prevalence ofAnaplasmaspp. in Small Ruminants in Morocco

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