2020
DOI: 10.3390/pathogens9100857
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Molecular and Serological Prevalence of Leptospira spp. in Feral Pigs (Sus scrofa) and their Habitats in Alabama, USA

Abstract: Leptospirosis is a widespread zoonosis and has been recognized as a re-emerging infectious disease in humans and a variety of wild and domestic animal species. In order to understand the prevalence and diversity of Leptospira spp. in feral pig populations of Alabama, we trapped 315 feral pigs in Bullock County east-central Alabama, and collected 97 environmental samples from riparian areas in Bullock County and Macon County east-central Alabama. Two previously published PCRs followed by DNA sequencing and BLAS… Show more

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Cited by 6 publications
(5 citation statements)
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“…Feral animals change the biodiversity in a region and the prevalence of reservoir hosts for leptospirosis, potentially shedding leptospires in the environment. Several states in the US have documented increases in feral swine in the last decade, with seroprevalence indicating past or present leptospiral infections in approximately 50% of populations in some areas [79][80][81][82][83][84][85]. The wildlife trade can also change biodiversity by introducing nonnative species and foreign pathogens into the United States [86].…”
Section: Canine Importation Feral Animals and Wildlife Tradementioning
confidence: 99%
“…Feral animals change the biodiversity in a region and the prevalence of reservoir hosts for leptospirosis, potentially shedding leptospires in the environment. Several states in the US have documented increases in feral swine in the last decade, with seroprevalence indicating past or present leptospiral infections in approximately 50% of populations in some areas [79][80][81][82][83][84][85]. The wildlife trade can also change biodiversity by introducing nonnative species and foreign pathogens into the United States [86].…”
Section: Canine Importation Feral Animals and Wildlife Tradementioning
confidence: 99%
“…After discarding the ethanol, the mosquito was rinsed in fresh 1× PBS four times and homogenized with 400 µl of 1× PBS using three zirconia beads in a Precellys 24 lysis and homogenization instrument (Bertin Instruments, France) set at 5000 rpm for 15 s as described [ 36 ]. Homogenates were stored at −20 °C until DNA extraction using the High Pure PCR Template Preparation Kit (Roche Diagnostics, Indianapolis, IN, USA) as described previously [ 46 , 47 ].…”
Section: Methodsmentioning
confidence: 99%
“…Between July 2019 and March 2020, feral swine (n = 276) were trapped at a 4515-hectare privately-owned land in Bullock County in Alabama as described [ 32 ]. The property lies within the Upper Coastal Plain physiographic region.…”
Section: Methodsmentioning
confidence: 99%
“…The High-Pure PCR Template Preparation Kit (Roche Diagnostics, Indianapolis, IN USA) was used to extract total nucleic acids from whole blood according to the manufacturer’s instructions and described previously [ 11 , 32 ]. In brief, whole blood (400 µL) was mixed with equal volume of binding buffer followed by homogenization and digestion with proteinase K (10% of total volume).…”
Section: Methodsmentioning
confidence: 99%