Molecular and structural characterization of the biosurfactant produced by Pseudomonas aeruginosa DAUPE 614

Monteiro, Safi Amaro; Sassaki, Guilherme L.; Souza, Lauro Mera de; Meira, Joel Alexandre; Araújo, Janete Magalí de; Mitchell, David A.; Ramos, Luiz Pereira; Krieger, Nádia

doi:10.1016/j.chemphyslip.2007.02.001

Cited by 144 publications

(78 citation statements)

References 30 publications

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“…About 3.2 g/L of RHL has been achieved with those low cost materials [55]. Another report from Monteiro et al [57] suggested that about 3.9 g/L of RHL biosurfactant can be achieved from P. aeruginosa DAUPE 614 in a culture medium supplemented with glycerol (sole carbon source) and ammonium nitrate (nitrogen) where C:N ratio of 55:1.Patel and Desai [35] had used P. aeruginosa GS3 for production of RHL by growing culture in a medium supplemented with molasses (7% v/v) and CSL (0.5% v/v). Olive Oil Mills (OOM) produces concentrated black liquor syrup (water-soluble portion of ripe olives) which is one of the major concerns for agriculture sector.…”

Section: Rhamnolipid (Rhl)mentioning

confidence: 93%

Biosurfactants’ Production from Renewable Natural Resources: Example of Innovativeand Smart Technology in Circular Bioeconomy

Satpute

Płaza

Banpurkar

2017

Management Systems in Production Engineering

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Abstract:A strong developed bio-based industrial sector will significantly reduce dependency on fossil resources, help the countries meet climate change targets, and lead to greener and more environmental friendly growth. The key is to develop new technologies to sustainably transform renewable natural resources into bio-based products and biofuels. Biomass is a valuable resource and many parameters need to be taken in to account when assessing its use and the products made from its. The bioeconomy encompass the production of renewable biological resources and their conversion into food, feed and bio-based products (chemicals, materials and fuels) via innovative and efficient technologies provided by industrial biotechnology. The paper presents the smart and efficient way to use the agro-industrial, dairy and food processing wastes for biosurfactant's production. Clarification processes are mandatory to use the raw substrates for microbial growth as well as biosurfactant production for commercial purposes. At the same time it is very essential to retain the nutritional values of those cheap substrates. Broad industrial perspectives can be achieved when quality as well as the quantity of the biosurfactant is considered in great depth. Since substrates resulting from food processing, dairy, animal fat industries are not explored in great details; and hence are potential areas which can be explored thoroughly.

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Section: Rhamnolipid (Rhl)mentioning

confidence: 93%

Biosurfactants’ Production from Renewable Natural Resources: Example of Innovativeand Smart Technology in Circular Bioeconomy

Satpute

Płaza

Banpurkar

2017

Management Systems in Production Engineering

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“…However, only a small proportion were biosurfactant producers, which support the concept of bacterial community, whereas the biosurfactant producers will liberate the surface-tension-active molecules, that will help other microorganisms to degrade the wide variety of hydrocarbons found at the site (28, 30). has also been reported that the most known biosurfactant that they produce, is a rhamnolipid (22,33). Although most of the reports on rhamnolipid production are attributed to…”

Section: Screening Of Biosurfactant Producing Bacteriamentioning

confidence: 99%

Selection of biosurfactan/bioemulsifier-producing bacteria from hydrocarbon-contaminated soil

Viramontes-Ramos¹,

M²,

Ballinas-Casarrubias³

et al. 2010

Braz. J. Microbiol.

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Petroleum-derived hydrocarbons are among the most persistent soil contaminants, and some hydrocarbondegrading microorganisms can produce biosurfactants to increase bioavailability and degradation. The aim of this work was to identify biosurfactant-producing bacterial strains isolated from hydrocarboncontaminated sites, and to evaluate their biosurfactant properties. The drop-collapse method and minimal agar added with a layer of combustoleo were used for screening, and positive strains were grown in liquid medium, and surface tension and emulsification index were determined in cell-free supernantant and cell suspension. A total of 324 bacterial strains were tested, and 17 were positive for the drop-collapse and hydrocarbon-layer agar methods. Most of the strains were Pseudomonas, except for three strains (Acinetobacter, Bacillus, Rhodococcus). Surface tension was similar in cell-free and cell suspension measurements, with values in the range of 58 to 26 (mN/m), and all formed stable emulsions with motor oil (76-93% E 24 ). Considering the variety of molecular structures among microbial biosurfactants, they have different chemical properties that can be exploited commercially, for applications as diverse as bioremediation or degradable detergents.

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“…Several structural rhamnolipid congeners produced by different strains of P. aeruginosa have been identified so far (22,(30)(31)(32).The type of rhamnolipid produced depends on the bacterial strain, the carbon source used, and the process strategy (24,33,34). Rhamnolipids are formed by one or two rhamnose molecules linked to one or two fatty acids of saturated or unsaturated alkyl chain between C 8 and C 12 (35).…”

Section: Bagheri Lotfabad T Et Al Antibacterial Capabilities Of Rhammentioning

confidence: 99%

Assessment of Antibacterial Capability of Rhamnolipids Produced by Two Indigenous Pseudomonas aeruginosa Strains

Lotfabad

Shahcheraghi

Shooraj

2012

Jundishapur J Microbiol

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This study shows that rhamnolipid mixtures of MR01 and MASH1 have antimicrobial efficacy against Gram positive bacteria and may be evaluated as antimicrobial agent against multidrugresistant clinical pathogenic isolates. Background:In recent decades, biological properties of biosurfactants, particularly glycolipids, including antimicrobial, antifungal, and anti-cellular effects have been projected in many research studies. The unique advantages of these compounds in terms of production and environment made them important as antimicrobial agents. Objectives: This study aims to evaluate probable antibacterial and antifungal properties of MR01 and MASH1 biosurfactants against several specified microorganisms. Materials and Methods:In this article antibacterial activities of two rhamnolipid mixtures of MR01 and MASH1 were studied. MR01 and MASH1 are glycolipid-type biosurfactants that are being produced by two strains of Pseudomonas aeruginosa MR01 and MASH1, respectively. Antibacterial effects of two biosurfactants were assessed by disc diffusion test method and determination of minimum inhibitory concentration (MIC). Results: They exhibit not only excellent surface activity but also remarkable inhibitory effect against Gram-positive bacteria. According to results, although none of two biosurfactants showed significant effects on Gram negative bacteria growth inhibition, both assessing methods confirmed the growth inhibition of Gram-positive bacteria by MR01 and MASH1 biosurfactants. Conclusions: According to results of this study, MR01 and MASH1 biosurfactants had antimicrobial efficacy against Gram positive bacterial groups. This effect is comparable to antibiotics and therefore the future use of these biosurfactants as broad spectrum antibiotics is highly promising. These features of biosurfactants (BS) should broaden its applications in new advanced technologies. Future studies will be performed on characterization and isolation of biologically active fraction of the rhamnolipid biosurfactant produced by P. aeruginosa strains. This bioactive compound may be evaluated as a potent antimicrobial agent to be applied against a panel of pathogenic micro-organisms including a few multidrug-resistant (MDR) clinical pathogenic isolates such as methicillin-resistant Staphylococcus aureus (MRSA) and other MDR pathogenic strains.

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Molecular and structural characterization of the biosurfactant produced by Pseudomonas aeruginosa DAUPE 614

Cited by 144 publications

References 30 publications

Biosurfactants’ Production from Renewable Natural Resources: Example of Innovativeand Smart Technology in Circular Bioeconomy

Biosurfactants’ Production from Renewable Natural Resources: Example of Innovativeand Smart Technology in Circular Bioeconomy

Selection of biosurfactan/bioemulsifier-producing bacteria from hydrocarbon-contaminated soil

Assessment of Antibacterial Capability of Rhamnolipids Produced by Two Indigenous Pseudomonas aeruginosa Strains

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