Molecular and virulence characterization of highly prevalent Streptococcus agalactiae circulated in bovine dairy herds

Pang, Maoda; Sun, Lichang; He, Tao; Bao, Hongdu; Zhang, Lili; Zhou, Yan; Zhang, Hui; Wei, Ruicheng; Liu, Yongjie; Wang, Ran

doi:10.1186/s13567-017-0461-2

Cited by 43 publications

(53 citation statements)

References 51 publications

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“…In this case, in most cases, the etiology of mastitis is multifactorial and the disease always arises on the background of a decrease in immunobiological protection, both at the local and system level, and is accompanied by changes in immune reactivity (Hamilton et al, 2006;Wu et al, 2015). However, the microbial factor is of paramount importance in the application of the pathogenetic mechanisms of this pathology, and therefore knowledge and determination of the biological balance between microorganisms and microorganisms is a significant moment in improving the methods of diagnosis, prevention and treatment of cows Pang et al, 2017).…”

Section: Tablementioning

confidence: 99%

“…Despite this, the researchers constantly encountered problems in interpreting the results (Singh et al, 2011;Wang et al, 2014;Al-Farha et al, 2017;Sato et al, 2017). It was related to the of dynamic changes in the quantitative composition of somatic cells in different periods of lactation, daily oscillations in their numbers; study species and individual peculiarities of animals (Zhelavskyi, 2005;Kurjogi et al, 2014;Lombardini et al, 2017;Pang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Changes in cellular factors of local immunity udder of cows with mastitis

2019

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Zhelavskyi, M.M., Dmytriv, O.Ya., & Mizyk, V.P. (2019). Changes in cellular factors of local immunity udder of cows with mastitis. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. Series: Veterinary sciences, 21(93), 46-52. doi: 10.32718/nvlvet9309The immunological aspects of lactation of cows are investigated in this work. The authors present modern scientific data on the local immune protection of the mammary gland of cows. The purpose of the research was to improve the laboratory methods of diagnosis of mastitis and to study the cytological composition of the secretion of the breast of the cows for subclinical and purulent catarrhal inflammation of the udder. The cytological differentiation of milk cells was carried out according to its own developed, tested and patented method. Our experimental data convincingly confirm that in subclinical and purulentcatarrhal mastitis in cows there are significant changes in changes in the cytological composition and the system of local immune defense of the mammary gland. An important role in inflammation of the mammary gland is played by neutrophilic granulocytes that migrate to the parenchyma at the beginning of the clinical pathology (with subclinical inflammation) and release a range of inflammatory mediators in the inflammation zone. Subclinical mastitis of cows is accompanied by a sharp increase in the total somatic cells count in the secretion of the mammary gland. This is due to the activity of neutrophilic granulocytes, monocytes of histiocytes. Corresponding changes were found in the cytological balance of immunocompetent cells of the secretion of cows at from subclinical mastitis. The most significant shift was noted in the index of migratory activity of neutrophils (P < 0.01), which is a specific "indicator" indicator of inflammatory response in the body of diseased animals. Subclinical inflammation was also manifested by a decrease in the migration activity of lymphocytes. At purulent-catarrhal mastitis of cows there is a significant increase in the number of somatic cells. Also, the migration activity of neutrophils and histiocytes in the pathologic process zone is increasing and changes in the balance of immune cells are established. Thus, our experimental data convincingly confirm that in subclinical and purulent-catarrhal mastitis in cows there are significant changes in the system of local immune defense of the mammary gland. It has an important diagnostic value and also needs immunocorrection in the treatment process.

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Section: Tablementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Changes in cellular factors of local immunity udder of cows with mastitis

2019

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“…Immunobiological protection of the body is provided by a system of organs, tissues and cells that interact with pathogenic factors: bacteria, viruses, mycoplasmas, rickettsiae, fungi, parasites and ensure their destruction and elimination from the body. At the same time, the immune system carries out censorship control in the body recognizes and neutralizes moused, invaded, apoptotic cells and cells with signs of oncogenic transformation (Vernay et al, 2012;Tizard, 2013;Kurjogi & Kaliwal, 2014;Pang et al, 2017;Ruegg, 2017).…”

Section: Resultsmentioning

confidence: 99%

Immunobiological aspects of cow lactation

Zhelavskyi

2019

SMLNUVMB

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Lactation of cows is a high-energy metabolic process. It is regulated in the body by the complex physiological mechanisms of the nervous, endocrine and immune systems. The purpose of to study changes in the immunobiological reactivity of the body of cows during lactation. Clinical and experimental studies were accomplished at the veterinary clinic and in the specialized laboratory of reproductive animal immunology. For the study, four groups of analogues of experimental animals were formed in which the immunological methods determined the immune status: cows (n = 17) during the early lactation (secretion of colostrum, 3–5th day); second (n = 32) – cows (n = 32) in the mid (3–5th month) lactation; the third (n = 28) – during the late lactation (5–7th day) and the fourth group (n = 28) – during the dry period (12–20th day). Our research has shown that during lactation in the body of cows fluctuations of the studied indicators of cellular and humoral immunity occur. Experimental data that showed that the percentage of T-lymphocytes in the blood of cows of Ukrainian dairy black-billed breed during the initial (3–5 days) period of lactogenesis is the lowest (37.88 ± 1.53%). However, the lowest expression of B-immune cells (16.05 ± 0.74%) was also detected during colostrum secretion. These changes occurred against the background of the decrease in CD3+/СD22+ (2.36 ± 0.11). In particular, at the 3–5th month of lactation, peripheral blood flow contained 53.40 ± 0.83% CD3+, ie 1.40 times more. The number of CD22+ mononuclear cells increased by only 1.14 times (up to 18.31 ± 0.69%). The beginning late lactation and dry period in the number of immunocompetent cells was observed: CD3+ – up to 42.11 ± 1.03%, and CD22+ – up to 22.92 ± 0.89%. All this was accompanied by a corresponding redistribution of the balance (CD3+/СD22+). We have identified certain patterns in the blast transformation ability of T-lymphocytes, which was manifested in the change in the activity of activated immune cells of blood. At the beginning of lactation, the functional capacity of T cells was the lowest (42.47 ± 0.62%). But subsequently with the increase of the lactation curve there was a certain activation of their transformation into blasts (51.11 ± 1.05%). At the beginning of late lactation cows underwent a gradual decrease in the blasttransformation capacity of immunocompetent cells (43.11 ± 0.89%). And which continued to decline gradually during the dry period. Thus, during lactation in the body of cows there are dynamic permanent changes in the system of cellular and humoral immunity. From the beginning of lactogenesis, there is a gradual increase in the increase in the functioning of the mammary gland of cows, and further decrease in the population composition of CD3+ and СD22+, which was also accompanied by changes in the functional activity of immunocompetent cells.

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“…Genetic diversity and clonal relatedness of S. agalactiae strains recovered from human and animal infections have been previously demonstrated by pulsed-field gel electrophoresis (PFGE) (Pinto et al 2013). Studies performed in different countries showed capsular types Ia, II, III, IV and V predominant in human and bovine infections, including Brazil (Pinto et al 2013;Carvalho-Castro et al 2017;Pang et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Biofilm formation on different pH conditions byStreptococcus agalactiaeisolated from bovine mastitic milk

Miranda

Lannes-Costa

Pimentel

et al. 2018

Lett Appl Microbiol

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Streptococcus agalactiae is among the most relevant aetiologic agent of bovine clinical and subclinical mastitis, a major problem for the dairy industry. The disease may cause significant economic loss due to decreased production and milk quality and increased use of medicaments. Presently, data demonstrated that biofilm formation favours the establishment of infectious process in health mammary tissue by S. agalactiae and emphasizes that an acidic pH promotes adhesion by biofilm-forming bacterial strains. S. agalactiae strains (25%) showed resistance to tetracycline, azithromycin, erythromycin and clindamycin, and consequently were classified as multidrug-resistant strains.

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Molecular and virulence characterization of highly prevalent Streptococcus agalactiae circulated in bovine dairy herds

Cited by 43 publications

References 51 publications

Changes in cellular factors of local immunity udder of cows with mastitis

Changes in cellular factors of local immunity udder of cows with mastitis

Immunobiological aspects of cow lactation

Biofilm formation on different pH conditions byStreptococcus agalactiaeisolated from bovine mastitic milk

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