Molecular Aspects of core-shell Intrinsic Defect Induced Enhanced Antibacterial Activity of ZnO Nanocrystals

Verma, Suresh K.; Jha, Ealisha; Panda, Pritam Kumar; Das, Jugal Kishore; Thirumurugan, Arun; Suar, Mrutyunjay; Parashar, S. K. S.

doi:10.2217/nnm-2017-0237

Cited by 86 publications

(37 citation statements)

References 105 publications

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“…Synthesis of metallic oxide nanoparticles such as ZnO has been reported by HEBM in previous studies. 47 Similar changes in physiochemical properties of ZnO nanoparticles have been reported in the case of zinc oxide in the previous literature reported by our group and other groups. 47 − 50 These results have revealed the change in physiochemical properties of TiO 2 nanoparticles with milling time and speculated their change in the biological effect accordingly.…”

Section: Discussionsupporting

confidence: 89%

Mechanistic Insight into Size-Dependent Enhanced Cytotoxicity of Industrial Antibacterial Titanium Oxide Nanoparticles on Colon Cells Because of Reactive Oxygen Species Quenching and Neutral Lipid Alteration

Verma¹,

Jha

Panda³

et al. 2018

ACS Omega

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This study evaluates the impact of industrially prepared TiO2 nanoparticles on the biological system by using an in vitro model of colon cancer cell lines (HCT116). Industrial synthesis of titanium oxide nanoparticles was mimicked on the lab scale by the high-energy ball milling method by milling bulk titanium oxide particles for 5, 10, and 15 h in an ambient environment. The physiochemical characterization by field emission scanning electron microscopy, dynamic light scattering, and UV–visible spectroscopy revealed alteration in the size and surface charge with respect to increase in the milling time. The size was found to be reduced to 82 ± 14, 66 ± 12, and 42 ± 10 nm in 5, 10, and 15 h milled nano TiO2 from 105 ± 12 nm of bulk TiO2, whereas the zeta potential increased along with the milling time in all biological media. Cytotoxicity and genotoxicity assays performed with HCT116 cell lines by MTT assay, oxidative stress, intracellular lipid analysis, apoptosis, and cell cycle estimation depicted cytotoxicity as a consequence of reactive oxygen species quenching and lipid accumulation, inducing significant apoptosis and genotoxic cytotoxicity. In silico analysis depicted the role of Sod1, Sod2, p53, and VLDR proteins–TiO2 hydrogen bond interaction having a key role in determining the cytotoxicity. The particles exhibited significant antibacterial activities against Escherichia coli and Salmonella typhimurium.

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Section: Discussionsupporting

confidence: 89%

Mechanistic Insight into Size-Dependent Enhanced Cytotoxicity of Industrial Antibacterial Titanium Oxide Nanoparticles on Colon Cells Because of Reactive Oxygen Species Quenching and Neutral Lipid Alteration

Verma¹,

Jha

Panda³

et al. 2018

ACS Omega

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“…Size, shape, and morphology of the metal nanoparticles are strongly influenced by the metabolites in the plant extract, which leads to the formation of nanoparticles of high stability with narrow size distribution [ 86 , 87 ]. Plant metabolites are terpenoids, polyphenols, sugars, alkaloids, phenolic acid, and proteins which play an important role in the binding and reduction of metal ions, thus thereby yielding metal nanoparticles as shown in Fig.…”

Section: Various Techniques For Nanoparticle Synthesismentioning

confidence: 99%

Nanotechnology and its challenges in the food sector: a review

Kumar

Mahajan

Kaur

et al. 2020

Materials Today Chemistry

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Antibacterial activity of nanoparticles has received significant attention worldwide because of their great physical and chemical stability, excellent magnetic properties, and large lattice constant values. These properties are predominate in the food science for enhancing the overall quality, shelf life, taste, flavor, process-ability, etc., of the food. Nanoparticles exhibit attractive antibacterial activity due to their increased specific surface area leading to enhanced surface reactivity. When nanoparticles are suspended in the biological culture, they encounter various biological interfaces, resulting from the presence of cellular moieties like DNA, proteins, lipids, polysaccharides, etc., which helps antibacterial properties in many ways. This paper reviews different methods used for the synthesis of nanoparticles but is specially focusing on the green synthesis methods owing to its non-toxic nature towards the environment. This review highlights their antibacterial application mainly in the food sector in the form of food-nanosensors, food-packaging, and food-additives. The possible mechanism of nanoparticles for their antibacterial behavior underlying the interaction of nano-particles with bacteria, (i) excessive ROS generation including hydrogen peroxide (H 2 O 2 ), OH − (hydroxyl radicals), and O − 2 2 (peroxide); and (ii) precipitation of nano-particles on the bacterial exterior; which, disrupts the cellular activities, resulting in membranes disturbance. All these phenomena results in the inhibition of bacterial growth. Along with this, their current application and future perspectives in the food sector are also discussed. Nanoparticles help in destroying not only pathogens but also deadly fungi and viruses. Most importantly it is required to focus more on the crop processing and its containment to stop the post-harvesting loss. So, nanoparticles can act as a smart weapon towards the sustainable move.

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“…Some works have evidenced ZnO nanoparticles with dimensions smaller than 100 nm have shown increasing antimicrobial properties against Gram-negative and Gram-positive bacteria due to a higher cellular internalization. Specifically, Verma et al (2018) studies have indicated that the antibacterial activity of ZnO nanoparticles against Staphylococcus aureus and Escherichia coli increased with the decrease in their size from 250 to 80, 40, and 20 nm due to an increased reactive oxygen species generation and membrane damage in bacteria [11,12]. Different synthesis methods as sol-gel, hydrothermal, simple thermal sublimation, vapor-liquid-solid, double-jet precipitation, self-combustion, and "green synthesis" have been used to obtain ZnO nanorods, nanospheres, nanotubes, nanowires, and nanoneedles [13][14][15].…”

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confidence: 99%

Antimicrobial Bilayer Nanocomposites Based on the Incorporation of As-Synthetized Hollow Zinc Oxide Nanotubes

et al. 2020

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An antimicrobial polymeric bilayer structure based on the application of an acrylic coating containing hollow zinc oxide nanotubes over a polymeric substrate was developed in this work. Firstly, zinc oxide nanotubes (ZnO NT ) were obtained by an atomic layer deposition (ALD) process over electrospun polyvinyl alcohol nanofibers followed by polymer removal through calcination with the purpose of obtaining antimicrobial nanostructures with a high specific area. Parameters of electrospinning, ALD, and calcination processes were set in order to obtain successfully hollow zinc oxide nanotubes. Morphological studies through scanning electron microscopy (SEM) and transmission electron microscopy (TEM) microscopies confirmed the morphological structure of ZnO NT with an average diameter of 180 nm and thickness of approximately 60 nm. Thermal and X-ray diffraction (XRD) analyses provided evidence that calcination completely removed the polymer, resulting in a crystalline hexagonal wurtzite structure. Subsequently, ZnO NT were incorporated into a polymeric coating over a polyethylene extruded film at two concentrations: 0.5 and 1 wt. % with respect to the polymer weight. An antimicrobial analysis of developed antimicrobial materials was performed following JIS Z2801 against Staphylococcus aureus and Escherichia coli. When compared to active materials containing commercial ZnO nanoparticles, materials containing ZnO NT presented higher microbial inhibition principally against Gram-negative bacteria, whose reduction was total for films containing 1 wt. % ZnO NT . Antiviral studies were also performed, but these materials did not present significant viral reduction.Nanomaterials 2020, 10, 503 2 of 14 antimicrobial nanostructures are one of the most promising alternatives in the search for new antimicrobial substances [3][4][5]. The attention has been principally centered on metallic/metal oxide nanoparticles due to their broad antibacterial and antifungal activities at low concentrations thanks to their high specific area [6][7][8][9].Zinc oxide (ZnO) nanoparticles are an inorganic material with optical, chemical sensing, electric conductivity, catalytic, photochemical, and antimicrobial properties. This metal oxide presents three crystal structures: wurtzite, zinc blend, and rocksalt, with a direct wide band gap of 3.3 Ev [10]. Some works have evidenced ZnO nanoparticles with dimensions smaller than 100 nm have shown increasing antimicrobial properties against Gram-negative and Gram-positive bacteria due to a higher cellular internalization. Specifically, Verma et al. (2018) studies have indicated that the antibacterial activity of ZnO nanoparticles against Staphylococcus aureus and Escherichia coli increased with the decrease in their size from 250 to 80, 40, and 20 nm due to an increased reactive oxygen species generation and membrane damage in bacteria [11,12]. Different synthesis methods as sol-gel, hydrothermal, simple thermal sublimation, vapor-liquid-solid, double-jet precipitation, self-combustion, and "green synthes...

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Molecular Aspects of core-shell Intrinsic Defect Induced Enhanced Antibacterial Activity of ZnO Nanocrystals

Abstract: Synthesized nano ZnO exhibit size-dependent antibacterial action as consequences of interactions with cell membrane proteins via hydrogen bond interaction with amino acid residues followed by internalization, membrane depolarization and induction of reactive oxygen species generation.

Cited by 86 publications

References 105 publications

Mechanistic Insight into Size-Dependent Enhanced Cytotoxicity of Industrial Antibacterial Titanium Oxide Nanoparticles on Colon Cells Because of Reactive Oxygen Species Quenching and Neutral Lipid Alteration

Mechanistic Insight into Size-Dependent Enhanced Cytotoxicity of Industrial Antibacterial Titanium Oxide Nanoparticles on Colon Cells Because of Reactive Oxygen Species Quenching and Neutral Lipid Alteration

Nanotechnology and its challenges in the food sector: a review

Antimicrobial Bilayer Nanocomposites Based on the Incorporation of As-Synthetized Hollow Zinc Oxide Nanotubes

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