Atherosclerosis IV 1977
DOI: 10.1007/978-3-642-95308-8_67
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Molecular Aspects of Hepatic Triglyceride Lipase and Lipoprotein Lipase from Human Post-Heparin Plasma

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Cited by 5 publications
(9 citation statements)
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“…Postheparin plasma was obtained from normal human volunteers 15 min after intravenous injection of 60 U/kg of heparin (Riker Laboratories, Northridge, CA) and from cynomolgus monkeys 10 min after 100 U/kg of heparin. Human HTGL was partially purified and anti-human HTCL antiserum was produced in a goat as previously reported (17). In vitro assay of human postheparin plasma demonstrated comparable levels of activity after pretreatment with the antibody or with 50 mM sodium dodecyl sulfate (18) using a gum-arabic stabilized emulsion of tri-(H3)oleoylglycerol (New England Nuclear, Boston, MA).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Postheparin plasma was obtained from normal human volunteers 15 min after intravenous injection of 60 U/kg of heparin (Riker Laboratories, Northridge, CA) and from cynomolgus monkeys 10 min after 100 U/kg of heparin. Human HTGL was partially purified and anti-human HTCL antiserum was produced in a goat as previously reported (17). In vitro assay of human postheparin plasma demonstrated comparable levels of activity after pretreatment with the antibody or with 50 mM sodium dodecyl sulfate (18) using a gum-arabic stabilized emulsion of tri-(H3)oleoylglycerol (New England Nuclear, Boston, MA).…”
Section: Methodsmentioning
confidence: 99%
“…The assay was initially developed using a homogenate of cynomolgus monkey liver prepared in buffer containing 0.01 M phosphate (pH 7.4) and 0.15 M NaCl (PBS), and 0.2 U/ml heparin. Lipolytic activity was assayed in aliquots of this homogenate using 0.45 ml of gum arabic emulsion containing 5 ,umol of trioleoylglycerol (10 ACi/ml) as previously described (17). Protein in the liver homogenate was measured by the method of Lowry et al (22) Immediately before injection, each preparation of IgG (prepared as described above) was filtered through a 0.45-,um filter (Millipore Corp., Bedford, MA) and centrifuged 2 h at 49,000 rpm in an SW50 rotor to remove aggregates.…”
Section: Methodsmentioning
confidence: 99%
“…Thus we concluded that the higher affinity, enzymatically active peak eluting from heparin-Sepharose may be present as a homodimer under conditions ofthe assay ( 18). However LPL is likely to dissociate into its subunits at NaCl concentrations of0.8 M or more (52)(53)(54). Therefore LPL may emerge from the column as a stable, dissociated monomer when it is eluted with -1.0 M NaCl, and then reassociate into the active dimer when it is diluted in the activity-assay buffer.…”
Section: Figs 3 B and 4 B)mentioning
confidence: 99%
“…From determinations by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), human HTGL with molecular weight of 62-69 [2,11,161, containing approximately 8% carbohydrate [2] has been described. From determinations by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), human HTGL with molecular weight of 62-69 [2,11,161, containing approximately 8% carbohydrate [2] has been described.…”
Section: Introductionmentioning
confidence: 99%