Molecular aspects of implantation

Aplin, John D.; Spanswick, Catherine; Behzad, Farhad; Kimber, Susan J.; Vićovac, Ljiljana

doi:10.1093/molehr/2.7.527

Cited by 80 publications

(16 citation statements)

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“…These receptor molecules facilitate the preferential entry of WNV and kunjin virus (a subtype of WNV) through the apical surface. Similarly, apical localization of ␣ v and ␤ 3 integrin subunits expression was observed in polarized cells (35). This provided further evidence of the specificity of WNV for ␣ v ␤ 3 integrin.…”

Section: Discussionsupporting

confidence: 62%

Interaction of West Nile Virus with αvβ3 Integrin Mediates Virus Entry into Cells

Chu

2004

Journal of Biological Chemistry

199

161

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The functional receptor for the flavivirus West Nile (WNV) infection has been characterized in this study with a combination of biochemical and molecular approaches. A 105-kDa protease-sensitive glycoprotein that binds WNV was isolated from the plasma membrane of cells permissive to WNV infection. The protein was subjected to peptide sequencing, and this glycoprotein was identified as a member of the integrin superfamily. Infection of WNV was shown to be markedly inhibited in Vero cells pretreated with blocking antibodies against ␣ v ␤ 3 integrin and its subunits by receptor competition assay. It was also noted that cells pretreated with antibodies against ␣ v ␤ 3 integrin can effectively inhibit flavivirus Japanese encephalitis but to a lesser extent flavivirus dengue infections. West Nile virus entry is independent of divalent cations and is not highly blocked by arginine-glycine-aspartic acid (RGD) peptides, suggesting that the interaction between the virus and ␣ v ␤ 3 integrin is not highly dependent on the classical RGD binding motif. In addition, gene silencing of the ␤ 3 integrin subunit in cells has resulted in cells largely resistant to WNV infection. In contrast, expression of recombinant human ␤ 3 integrin substantially increased the permissiveness of CS-1 melanoma cells for WNV infection. Soluble ␣ v ␤ 3 integrin can also effectively block WNV infection in a dose-dependent manner. Furthermore, WNV infection also triggered the outside-in signaling pathway via the activation of integrin-associated focal adhesion kinase. The identification of ␣ v ␤ 3 integrin as a receptor for WNV provides insight into virusreceptor interaction, hence creating opportunities in the development of anti-viral strategies against WNV infection.

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Section: Discussionsupporting

confidence: 62%

Interaction of West Nile Virus with αvβ3 Integrin Mediates Virus Entry into Cells

Chu

2004

Journal of Biological Chemistry

199

161

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“…Indeed, chemokines have been implicated in a number of reproductive events, including ovulation, menstruation, embryo implantation, and parturition and disease conditions (i.e., endometriosis and cancer) [36,37]. ITGB5 (integrin beta 5) has also been implicated in cell migration and angiogenesis during embryo implantation due to its apical expression on glandular and luminal epithelial cells [38]. Previous research has demonstrated that the expression of ITGB5 , which regulated by chemokines (such as CX3CL1 and CCL14), could increase trophoblast adherence and migration at the maternal-fetal interface [39].…”

Section: Discussionmentioning

confidence: 99%

RUNX2, GPX3 and PTX3 gene expression profiling in cumulus cells are reflective oocyte/embryo competence and potentially reliable predictors of embryo developmental competence in PCOS patients

Huang

Hao

Shen

et al. 2013

Reprod Biol Endocrinol

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BackgroundPolycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The developmental competence of oocytes and embryos in PCOS patients is reduced to a certain extent (comparing to non-PCOS patients, the high quality embryo rate was decreased by 16% from the data of our centre) during the in vitro fertilization (IVF) process. Cross-talk between the oocyte and cumulus cells is critical for oocyte maturation and embryo competence. In this study, we have evaluated the transcription of specific genes in cumulus cells harvested from pre-ovulatory follicles of PCOS patients before IVF, according to individual oocyte nuclear maturity and developmental competence. Seven genes (RUNX2, PSAT1, ADAMTS9, CXCL1, CXCL2, CXCL3, and ITGB5) were targeted from our previous cDNA microarray data which isolated genes related to oocyte nuclear maturation in PCOS patients. Two additional genes which had been found to be associated with oocyte maturation or embryo quality in non-PCOS patients (GPX3 and PTX3) were also studied.MethodsThe mRNA expression levels of cumulus cells were detected by qRT- PCR.ResultsConsistent with our previous cDNA microarray data, with the exception of GPX3 and PTX3, the selected 7 genes were related to oocyte nuclear maturation in PCOS patients. Noticeably, the expression level of RUNX2 was lower in cumulus cells derived from oocytes that could develop into blastocysts than the level of expression from oocytes that could not. The PTX3 expression level was significantly lower in cumulus cells from oocytes with two normal pronuclei than that from oocytes that formed >2 pronuclei (MPN) after fertilization. GPX3 mRNA levels were decreased in cumulus cells isolated from oocytes that developed into blastocysts with high potential development competence.ConclusionsSeveral cumulus cell genes were associated with oocyte maturation, fertilization and embryo quality in PCOS patients. RUNX2 and GPX3 are candidate genetic markers in the monitoring of embryo quality for PCOS patients, whereas PTX3 mainly played a role in fertilization process. Together with morphological evaluation, cumulus cells genes may serve as biomarkers of oocyte and embryo selection during the IVF process for PCOS patients and may advance our understanding of PCOS.

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“…Indeed, it has been demonstrated that the aberrant expression of integrin αvβ3 is associated with infertility and recurrent pregnancy loss (Lessey et al, 1995; Liu et al, 2012). In mice, αvβ3 is expressed in the uterine luminal epithelium and the blastocyst during implantation, and injection of RGD peptides or neutralizing antibody against the αv or β3 subunit into the mouse uterine cavity reduces the rate of implantation compared to injection of BSA or non-RGD peptides, suggesting its participation in mediating the trophectoderm-luminal epithelium interaction (Aplin et al, 1996; Illera et al, 2000; Sutherland et al, 1993). There is evidence that integrin signaling modulates trophoblast adhesion to extracellular matrices via activation of phospholipase C-γ to initiate phosphoinositide signaling and intracellular calcium mobilization during blastocyst implantation (Wang et al, 2002, 2007b).…”

Section: Cell-cell Interactions: the Nature Of Embryo Implantationmentioning

confidence: 99%

Physiological and molecular determinants of embryo implantation

Zhang

Lin

Kong

et al. 2013

Molecular Aspects of Medicine

450

480

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Embryo implantation involves the intimate interaction between an implantation-competent blastocyst and a receptive uterus, which occurs in a limited time period known as the window of implantation. Emerging evidence shows that defects originating during embryo implantation induce ripple effects with adverse consequences on later gestation events, highlighting the significance of this event for pregnancy success. Although a multitude of cellular events and molecular pathways involved in embryo-uterine crosstalk during implantation have been identified through gene expression studies and genetically engineered mouse models, a comprehensive understanding of the nature of embryo implantation is still missing. This review focuses on recent progress with particular attention to physiological and molecular determinants of blastocyst activation, uterine receptivity, blastocyst attachment and uterine decidualization. A better understanding of underlying mechanisms governing embryo implantation should generate new strategies to rectify implantation failure and improve pregnancy rates in women.

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Molecular aspects of implantation

Cited by 80 publications

References 0 publications

Interaction of West Nile Virus with αvβ3 Integrin Mediates Virus Entry into Cells

Interaction of West Nile Virus with αvβ3 Integrin Mediates Virus Entry into Cells

RUNX2, GPX3 and PTX3 gene expression profiling in cumulus cells are reflective oocyte/embryo competence and potentially reliable predictors of embryo developmental competence in PCOS patients

Physiological and molecular determinants of embryo implantation

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