Molecular Aspects of Plant Virus Transmission by Olpidium and Plasmodiophorid Vectors

Rochon, D’Ann; Kakani, Kishore; Robbins, Marjorie; Reade, Ron

doi:10.1146/annurev.phyto.42.040803.140317

Cited by 97 publications

(90 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this unit cell, there was a single icosahedral penton in the crystallographic asymmetric unit. Using the self-rotation function in the CCP4 suite as a guide (15), the h, k, and l indexing was consistent with the icosahedral setting used by the Viper database (http://viperdb.scripps.edu): z(2)-3-5-x (2). In this orientation, the two icosahedral 2-fold axes coincide with the z-and x-coordinate axes, while a set of icosahedral 3-fold and 5-fold axes lie, in that order, between the z and x axes in the x-z plane.…”

Section: Methodsmentioning

confidence: 99%

“…ucumber necrosis virus (CNV) is a member of the genus Tombusvirus and has a monopartite positive-sense RNA genome (1,2). CNV is transmitted in nature via zoospores of the fungus Olpidium bornovanus (2)(3)(4).…”

mentioning

confidence: 99%

“…CNV is transmitted in nature via zoospores of the fungus Olpidium bornovanus (2)(3)(4). The type species of the Tombusvirus genus is Tomato bushy stunt virus (TBSV), and the structure of TBSV was the first virus structure determined by X-ray crystallography (5).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Atomic Structure of Cucumber Necrosis Virus and the Role of the Capsid in Vector Transmission

Kakani

Katpally

et al. 2013

J Virol

Self Cite

View full text Add to dashboard Cite

Cucumber necrosis virus (CNV) is a member of the genus Tombusvirus and has a monopartite positive-sense RNA genome packaged in a T‫3؍‬ icosahedral particle. CNV is transmitted in nature via zoospores of the fungus Olpidium bornovanus. CNV undergoes a conformational change upon binding to the zoospore that is required for transmission, and specific polysaccharides on the zoospore surface have been implicated in binding. To better understand this transmission process, we have determined the atomic structure of CNV. As expected, being a member of the Tombusvirus genus, the core structure of CNV is highly similar to that of Tomato bushy stunt virus (TBSV), with major differences lying on the exposed loops. Also, as was seen with TBSV, CNV appears to have a calcium binding site between the subunits around the quasi-3-fold axes. However, unlike TBSV, there appears to be a novel zinc binding site within the ␤ annulus formed by the N termini of the three C subunits at the icosahedral 3-fold axes. Two of the mutations causing defective transmission map immediately around this zinc binding site. The other mutations causing defective transmission and particle formation are mapped onto the CNV structure, and it is likely that a number of the mutations affect zoospore transmission by affecting conformational transitions rather than directly affecting receptor binding. C ucumber necrosis virus (CNV) is a member of the genusTombusvirus and has a monopartite positive-sense RNA genome (1, 2). CNV is transmitted in nature via zoospores of the fungus Olpidium bornovanus (2-4). The type species of the Tombusvirus genus is Tomato bushy stunt virus (TBSV), and the structure of TBSV was the first virus structure determined by X-ray crystallography (5).These viruses have a Tϭ3 icosahedral protein shell with a diameter of ϳ300 Å formed from 180 identical coat protein (CP) subunits. The three conformationally distinct copies of the CPs, called A, B, and C, are shown in Fig. 1. The C subunits lie next to the icosahedral 2-fold axes, while the A and B subunits surround the 5-fold axes. Each capsid protein is comprised of three domains: the first ϳ60 residues at the N-terminal region (R), the shell (S) domain, and the protruding (P) domain (Fig. 2A). The first ϳ90 residues are disordered in the A and B subunits, while the first ϳ60 residues are disordered in the C subunit. Residues ϳ60 to 78 in the C subunit form a structure called the ␤ annulus that is thought to stabilize the capsid. The first ϳ60 residues at the N-terminal region interact with the RNA interior. Between this region (R) and the S domain are ϳ30 residues that act as a flexible connecting arm. The S domain is ϳ170 residues and forms a tight protein shell around the RNA genome. The final ϳ115 residues form the P domains, which combine with the adjacent subunit's P domains to form dimeric protrusions from the shell. The A subunits lie immediately adjacent to the icosahedral 5-fold axes, and their P domains interact with the P domains of adjacent B subunits to form 60 of the 90...

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Atomic Structure of Cucumber Necrosis Virus and the Role of the Capsid in Vector Transmission

Kakani

Katpally

et al. 2013

J Virol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The true fungi of the species O. brassicae transmit viruses of the genera Ophiovirus and Varicosavirus. These viruses are transmitted in a mode referred to as 'in vivo', which resembles circulative transmission because the viruses enter into the cytoplasm of the vector, mostly zoospores from where they are released, presumably during germination [51].…”

Section: Ophiovirus Taxonomymentioning

confidence: 99%

Recent advances in Citrus psorosis virus

2014

View full text Add to dashboard Cite

“…A pesar de haberse demostrado dicha asociación, no se había estudiado hasta el momento la posible transmisión del virus mediante dicho hongo vector, responsable de la transmisión de otros virus que causan enfermedades importantes en otras especies hortícolas (Rochon et al, 2004;Sasaya y Koganezawa, 2006 brassicae que, correspondería a la cepa "crucífera" cuyo rango de hospedantes se restringe a dicha familia botánica y se caracteriza por necesitar el apareamiento entre zoosporas para el desarrollo de esporas de resistencia, y O. virulentus que constituiría la cepa "no crucífera" y no requeriría de la reproducción sexual para continuar su ciclo (Koganezawa et al, 2005;Sasaya y Koganezawa, 2006). En dos replicaciones distintas, se demostró la capacidad de O. virulentus, originario de un cultivo de tomate, para transmitir PepMV a plantas de tomate sanas regadas con el agua de drenaje recogida de plantas infectadas con ambos agentes.…”

Section: Discussionunclassified

Epidemiología y variabilidad patogénica del virus del mosaico del pepino dulce (Pepino mosaic virus). Nuevas enfermedades asociadas a su presencia (torrao o cribado)

Fernandez¹,

Olvido²

View full text Add to dashboard Cite

Molecular Aspects of Plant Virus Transmission by Olpidium and Plasmodiophorid Vectors

Cited by 97 publications

References 115 publications

Atomic Structure of Cucumber Necrosis Virus and the Role of the Capsid in Vector Transmission

Atomic Structure of Cucumber Necrosis Virus and the Role of the Capsid in Vector Transmission

Recent advances in Citrus psorosis virus

Epidemiología y variabilidad patogénica del virus del mosaico del pepino dulce (Pepino mosaic virus). Nuevas enfermedades asociadas a su presencia (torrao o cribado)

Contact Info

Product

Resources

About