Molecular Attenuation Process in Live Vaccine Generation for Arenaviruses

Goñi, Sandra Elizabeth; Lozano, Mario Enrique

doi:10.5772/33970

Point Mutation 2012

DOI: 10.5772/33970

|View full text |Cite

Molecular Attenuation Process in Live Vaccine Generation for Arenaviruses

Sandra Elizabeth Goñi¹,

Mario Enrique Lozano²

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2013

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Watching Every Step of the Way: Junín Virus Attenuation Markers in the Vaccine Lineage

Stephan

Lozano

Goñi

2013

View full text Add to dashboard Cite

The Arenaviridae family includes several hemorrhagic fever viruses which are important emerging pathogens. Junín virus, a member of this family, is the etiological agent of Argentine Hemorrhagic Fever (AHF). A collaboration between the Governments of Argentina and the USA rendered the attenuated Junín virus vaccine strain Candid#1. Arenaviruses are enveloped viruses with genomes consisting of two single-stranded RNA species (L and S), each carrying two coding regions separated by a stably structured, non-coding intergenic region. Molecular characterization of the vaccine strain and of its more virulent ancestors, XJ13 (prototype) and XJ#44, allows a systematic approach for the discovery of key elements in virulence attenuation. We show comparisons of sequence information for the S RNA of the strains XJ13, XJ#44 and Candid#1 of Junín virus, along with other strains from the vaccine lineage and a set of Junín virus field strains collected at the AHF endemic area. Comparisons of nucleotide and amino acid sequences revealed different point mutations which might be linked to the attenuated phenotype. The majority of changes are consistent with a progressive attenuation of virulence between XJ13, XJ#44 and Candid#1. We propose that changes found in genomic regions with low natural variation frequencies are more likely to be associated with the virulence attenuation process. We partially sequenced field strains to analyze the genomic variability naturally occurring for Junín virus. This information, together with the sequence analysis of strains with intermediate virulence, will serve as a starting point to study the molecular bases for viral attenuation.

show abstract

Watching Every Step of the Way: Junín Virus Attenuation Markers in the Vaccine Lineage

Stephan

Lozano

Goñi

2013

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Molecular Attenuation Process in Live Vaccine Generation for Arenaviruses

Cited by 1 publication

References 83 publications

Watching Every Step of the Way: Junín Virus Attenuation Markers in the Vaccine Lineage

Watching Every Step of the Way: Junín Virus Attenuation Markers in the Vaccine Lineage

Contact Info

Product

Resources

About