Molecular based assessment of genetic diversity within Barbary fig (Opuntia ficus indica (L.) Mill.) in Tunisia

Zoghlami, Nèjia; Chrita, Ichraf; Bouamama, Badra; Gargouri, Mahmoud; Zemni, Hassène; Ghorbel, Abdelwahed; Mliki, Ahmed

doi:10.1016/j.scienta.2007.02.009

Cited by 32 publications

(15 citation statements)

References 18 publications

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“…The characterization of material based on morphological traits and common local names often leads to duplicated accessions, many of which are synonymous or homonymous or have a false attribution (Zoghlami et al 2007). The practical utility of any molecular approach for germplasm management is partly determined by the ability to differentiate between large numbers of accessions (Gilbert et al 1999).…”

Section: Resultsmentioning

confidence: 99%

Analysis of Genetic Diversity of Persea bombycina “Som” Using RAPD-Based Molecular Markers

et al. 2009

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The utility of RAPD markers in assessing genetic diversity and phenetic relationships in Persea bombycina, a major tree species for golden silk (muga) production, was investigated using 48 genotypes from northeast India. Thirteen RAPD primer combinations generated 93 bands. On average, seven RAPD fragments were amplified per reaction. In a UPGMA phenetic dendrogram based on Jaccard's coefficient, the P. bombycina accessions showed a high level of genetic variation, as indicated by genetic similarity. The grouping in the phenogram was highly consistent, as indicated by high values of cophenetic correlation and high bootstrap values at the key nodes. The accessions were scattered on a plot derived from principal correspondence analysis. The study concluded that the high level of genetic diversity in the P. bombycina accessions may be attributed to the species' outcrossing nature. This study may be useful in identifying diverse genetic stocks of P. bombycina, which may then be conserved on a priority basis.

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Section: Resultsmentioning

confidence: 99%

Analysis of Genetic Diversity of Persea bombycina “Som” Using RAPD-Based Molecular Markers

et al. 2009

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“…The use of morphological descriptors comprising cladode, flower and fruit traits yielded a high number of morphotypes and allowed the discrimination of all of the studied species. In previous studies, the discrimination level was found to be as high as those reported by other studies using molecular markers for Opuntia spp (Labra et al 2003, García-Zambrano et al 2006, Zoghlami et al 2007, García-Zambrano et al 2009, Caruso et al 2010, Majure et al 2012, El-Banna et al 2013, Valadez-Moctezuma et al 2014, Ganopoulos et al 2015, Samah et al 2016.…”

Section: Discussionmentioning

confidence: 55%

“…The species reached the Mediterranean basin during the 16 th century (Barbera 1995). Nevertheless, only towards the end of the 20 th century have large plantations been established, where only rough estimates of the worldwide plantation area (Zoghlami et al 2007) are allowed due to the absence of updated statistical data from countries in which this species is cultivated.…”

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confidence: 99%

Polymorphism analysis in some Algerian <em>Opuntia</em> species using morphological and phenological UPOV descriptors

Hadjkouider¹,

Boutekrabt²,

Lallouche³

et al. 2017

Bot. Sci.

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Background: In the present study, we have investigated the morphological variation in a set of five Opuntia species from the Algerian steppes using 49 UPOV descriptors. Questions: Which of the 49 descriptors that can be used as powerful estimators of the phenotypic diversity within Opuntia species? How is the morphological diversity patterned in Algerian Opuntia? Species study/ Mathematical model: Opuntia ficus-indica, Opuntia amycleae, Opuntia streptacantha, Opuntia engelmannii, Opuntia robusta. Principal Components Analysis (PCA) and Hierarchical Cluster Analysis were used. Study site: Four counties were studied located in the Algerian steppes. The present research was carried out during 2014. Methods: 49 descriptors adopted by the International Union for the Protection of New Varieties of Plants (UPOV) were employed in the present research, where cladode, flower and fruit traits were used to determine the overall degree of polymorphism among 5 Opuntia species. Results: Principal Component Analysis and Hierarchical Cluster Analysis indicated a consistent differentiation between all studied species. The relative magnitude of the first two PCA eigenvectors showed that 8 descriptors out of 49 were identified as the most important descriptors for the classification of the species. The dendrogram performed on the calculated Euclidean distances between all species pairs allowed the identification of 3 groups, unlike the PCA that identified 4 groups. The species Opuntia ficus-indica and Opuntia amycleae were identified as very close morphologically. Conclusions:The present outcome represents a paramount step towards the fast selection of interesting species and for their best management and conservation. Key words: Opuntia, diversity, multivariate analyses, clustering, UPOV. ResumenAntecedentes: En el presente estudio, hemos investigado la variación morfológica en un conjunto de cinco especies de Opuntia de las estepas argelinas utilizando 49 descriptores UPOV. Preguntas: ¿Cuáles de los 49 descriptores que pueden ser utilizados como estimadores poderosos de la diversidad fenotípica dentro de las especies de Opuntia? ¿Cómo se modela la diversidad morfológica en el Opuntia Argelina? Especie en estudio/ Modelo matemático: Opuntia ficus-indica, Opuntia amycleae, Opuntia streptacantha, Opuntia engelmannii, Opuntia robusta. Análisis de Componentes Principales (PCA) y un Análisis de Agrupamiento Jerárquico fueron usados Sitio de estudio y fechas: Se estudiaron cuatro condados situados en la estepa argelina. La presente investigación se realizó durante el año 2014. Métodos: En la presente investigación se utilizaron 49 descriptores adoptados por la Unión Internacional para la Protección de las Obtenciones Vegetales (UPOV), en los que se utilizaron características de cladodio, flor y fruto para determinar el grado global de polimorfismo entre 5 especies de Opuntia. Resultados: Se aplicó un análisis multivariado, constituido por un análisis de componentes principales (PCA) y un análisis de agrupamiento jerárquico, cuyo...

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“…DNA amplification reactions and electrophoresis were performed according to Zoghlami et al (2007). Amplifications were repeated at least twice and only reproducible products were taken into account for further data analysis.…”

Section: Dna Amplificationmentioning

confidence: 99%

Comparative study of the growth parameters of legumes grown in fipronil-stressed soils

Ahemad¹,

Khan²

2011

Eurasia J Biosci

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For the first time, genetic variability and differentiation among five annual caraway (Carum carvi) populations originating from Tunisia, Germany and Egypt were examined. Random Amplified Polymorphic DNA (RAPD) marker data were obtained and analysed with respect to genetic diversity, population structure and gene flow. Fourteen primers generated a total of 136 discernible and reproducible bands across the analyzed populations, out of which 56 were polymorphic. The UPGMA cluster analysis permitted the discrimination of all the genotypes and their sorting into 3 main groups. Tunisian caraway populations diverged significantly from German and Egyptian ones. Population clustering was made dependently from geographic origin. This has been further explained at the DNA level as we were able to select a set of RAPD fingerprints unique to each of the studied populations. Furthermore, dimensional graph derived from factorial analysis of RAPD frequency data, allowed significant grouping of the genotypes into five sub-plots, representing each one population. Shannon's index values showed that variation ranks between rather than within populations. These results indicated that considerable genetic differences among C. carvi populations were registered.

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Molecular based assessment of genetic diversity within Barbary fig (Opuntia ficus indica (L.) Mill.) in Tunisia

Cited by 32 publications

References 18 publications

Analysis of Genetic Diversity of Persea bombycina “Som” Using RAPD-Based Molecular Markers

Analysis of Genetic Diversity of Persea bombycina “Som” Using RAPD-Based Molecular Markers

Polymorphism analysis in some Algerian <em>Opuntia</em> species using morphological and phenological UPOV descriptors

Comparative study of the growth parameters of legumes grown in fipronil-stressed soils

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