Molecular-based investigation of Cryptosporidium and Giardia from animals in water catchments in southeastern Australia

Nolan, Matthew J.; Jex, Aaron R.; Koehler, Anson V.; Haydon, Shane R.; Stevens, Melita; Gasser, Robin B.

doi:10.1016/j.watres.2012.12.027

Cited by 70 publications

(88 citation statements)

References 97 publications

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“…However, the IIaA19G2R1 subtype is a relatively rare subtype and has previously been described by Xiao et al (2007), Wielinga et al (2008), Ng et al (2012), Rieux et al (2013) and Couto et al (2014) infecting cattle in the North America, The Netherlands, Australia, France and Brazil, respectively and by Nolan et al (2013) in deer in Australia. To the best of our knowledge, this is the first report of this subtype in sheep.…”

Section: Discussionmentioning

confidence: 98%

Genetic characterization of Cryptosporidium in animal and human isolates from Jordan

Hijjawi

Mukbel

Yang

et al. 2016

Veterinary Parasitology

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(Mukbel R). #Joint first authors Highlights First genotyping study of Cryptosporidium in animals in Jordan  Faecal samples (n=284) from domestic animals and humans (n=48) screened  Overall prevalence of 11.6% in animals and six species detected.  Novel Cryptosporidium genotype detected in horses AbstractLittle is known about the epidemiology of Cryptosporidium in Jordan and to date, only one genotyping study has been conducted on Cryptosporidium isolates from Jordanian children. In the present study, a total of 284 faecal samples from Jordanian cattle, sheep, goats and chicken and 48 human faecal samples were screened for the presence of Cryptosporidium using an 18S quantitative PCR (qPCR) and a C. parvum/C. hominis specific qPCR at a lectin locus. Of these, 37 of 284 animal faecal samples were positive by qPCR at the 18S locus giving an overall prevalence of 11.6%. The point prevalence of Cryptosporidium in chickens, sheep, horses, cattle and goats ranged from 4.8% (chickens) to 18.7% (cattle). A total of six species were detected; C. xiaoi (n=9), C.andersoni (n=7), C. ryanae (n=5), C. parvum (n=4), C. baileyi (n=1) and a genetically distinct and potentially novel species in two isolates from horses. Sub-genotype analysis of the 4 C. parvum isolates at the 60-kDa glycoprotein (gp60) locus identified subtype IIaA19G2R1 (n=2) and IIaA16GR1 (n=2). For the human samples, 4 positives (8.3% prevalence) were detected. Of these, two were C. parvum (subtypes IIdA20G1 and IIaA15G2R1) and two were C. hominis (subtypes 1bA9G3 and 1bA10G2R2). Further studies are required to better understand the epidemiology and transmission of Cryptosporidium in Jordan.

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Section: Discussionmentioning

confidence: 98%

Genetic characterization of Cryptosporidium in animal and human isolates from Jordan

Hijjawi

Mukbel

Yang

et al. 2016

Veterinary Parasitology

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“…in wild deer, with C. ubiquitum, C. bovis, C. ryanae, the deer genotype and C. parvum and a C. hominis-like genotype reported (cf. Cinque et al 2008;Amer et al 2009;Feng, 2010;Ng et al 2011;Nolan et al 2013;García-Presedo et al 2013b). In captive cervids C. parvum and C. ubiquitum predominated (cf.…”

Section: Cryptosporidium In Wild Mammalsmentioning

confidence: 99%

“…Of the few genotyping studies conducted with foxes, the Cryptosporidium fox genotype, C. canis fox subtype (a variant of C. canis) and a Cryptosporidium macropodum-like genotype have been reported (Xiao et al 2002b;Ng et al 2011;Ruecker et al 2012Ruecker et al , 2013Nolan et al 2013).…”

Section: Cryptosporidium In Wild Mammalsmentioning

confidence: 99%

Cryptosporidiumspecies in humans and animals: current understanding and research needs

2014

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S U M M A R YCryptosporidium is increasingly recognized as one of the major causes of moderate to severe diarrhoea in developing countries. With treatment options limited, control relies on knowledge of the biology and transmission of the members of the genus responsible for disease. Currently, 26 species are recognized as valid on the basis of morphological, biological and molecular data. Of the nearly 20 Cryptosporidium species and genotypes that have been reported in humans, Cryptosporidium hominis and Cryptosporidium parvum are responsible for the majority of infections. Livestock, particularly cattle, are one of the most important reservoirs of zoonotic infections. Domesticated and wild animals can each be infected with several Cryptosporidium species or genotypes that have only a narrow host range and therefore have no major public health significance. Recent advances in next-generation sequencing techniques will significantly improve our understanding of the taxonomy and transmission of Cryptosporidium species, and the investigation of outbreaks and monitoring of emerging and virulent subtypes. Important research gaps remain including a lack of subtyping tools for many Cryptosporidium species of public and veterinary health importance, and poor understanding of the genetic determinants of host specificity of Cryptosporidium species and impact of climate change on the transmission of Cryptosporidium.

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“…Moreover, the gdh sequence from the red deer Giardia isolate also displayed 99% identity with the sequences of the same marker from cattle (K4016, DQ182607) in Denmark and had two single-nucleotide polymorphisms (SNPs) (Langkjaer et al 2007). Sequencing also showed that the tpi sequence was identical to the sequence of Giardia from deer (OS1704, KC282978) in Australia, red deer (C. elaphus) (JC002, (Lebbad et al 2010, Solarczyk et al 2012, Nolan et al 2013. Thus, the Giardia isolate from red deer RDP132 was designated as the (cervid-specific) sub-assemblage AIII.…”

mentioning

confidence: 94%

Axenic in vitro culture and molecular characterization of Giardia duodenalis from red deer (Cervus elaphus) and Thomson’s gazelle (Gazella thomsonii)

Solarczyk

Majewska

Słodkowicz-Kowalska

2014

Acta Parasitologica

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Giardia duodenalis is an ubiquitous flagellate that infects humans and many species of animals. This species exhibits great biotypic and genetic diversity. In the present study, we established short-and long-term in vitro cultures of G. duodenalis trophozoites originating from red deer and Thomson's gazelle (artiodactyls) and genetically characterised the isolates by their glutamate dehydrogenase and triose phosphate isomerase gene sequences. The G. duodenalis isolates from red deer and the gazelle represented assemblages A (AIII sub-assemblage) and B. In conclusion, G. duodenalis assemblages and sub-assemblages can be associated with differences in growth rate in vitro cultures.

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Molecular-based investigation of Cryptosporidium and Giardia from animals in water catchments in southeastern Australia

Cited by 70 publications

References 97 publications

Genetic characterization of Cryptosporidium in animal and human isolates from Jordan

Genetic characterization of Cryptosporidium in animal and human isolates from Jordan

Cryptosporidiumspecies in humans and animals: current understanding and research needs

Axenic in vitro culture and molecular characterization of Giardia duodenalis from red deer (Cervus elaphus) and Thomson’s gazelle (Gazella thomsonii)

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