Molecular Basis of Non-β-Lactam Antibiotics Resistance in Staphylococcus aureus

Lade, Harshad; Joo, Hwang-Soo; Kim, Jae‐Seok

doi:10.3390/antibiotics11101378

Cited by 26 publications

(14 citation statements)

References 276 publications

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“…Collectively, the drug resistance genes identified from our WGS results are consistent with various reported MDR-MRSA isolates that harbored determinants that enhanced their ability to resist antimicrobials [ 63 , 64 ]. These MDR-MRSA harbored blaZ -encoded β-lactamase to disrupt the amide bond of β-lactam ring and different families of exporters including efflux pumps such as NorA, SdrM, and LmrS to extrude drugs (e.g., macrolides, aminoglycosides, oxazolidinones, diaminopyrimidines, and phenicols) from the interior to the external environment, thus lowering their intracellular concentration [ 65 ].…”

Section: Discussionsupporting

confidence: 87%

Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Hospitalized Newborn Infants

et al. 2023

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Multidrug resistance (MDR) is a significant challenge in healthcare management, and addressing it requires a comprehensive approach. In this study, we employed a combination of phenotypic and genotypic approaches, along with whole genome sequencing (WGS) to investigate five hospital-associated MDR methicillin-resistant Staphylococcus aureus (MRSA) strains that were isolated from newborn infants. Our analysis revealed the following for the MDR-MRSA strains: SauR31 was resistant to three antimicrobial classes; SauR12, SauR91 and SauR110 were resistant to four antimicrobial classes; and SauR23 exhibited resistance to seven classes. All the MDR-MRSA strains were capable of producing slime and biofilms, harbored SCCmec type IV, and belonged to different spa types (t022, t032, and t548), with varying profiles for microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) and virulence genes. The WGS data for the MDR SauR23 and SauR91 strains revealed that most of the antimicrobial resistance genes were present in the chromosomes, including blaZ, mecA, norA, lmrS, and sdrM, with only the ermC gene found in a small (<3 kb) plasmid. The presence of MDR-MRSA strains among neonates raises public concern, hence implementation of multifaceted interventions is recommended to address this issue. In addition, metadata is needed to improve the investigation of antimicrobial resistance genes in MDR isolates.

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Section: Discussionsupporting

confidence: 87%

Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Hospitalized Newborn Infants

et al. 2023

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“…Staphylococcus aureus is a widespread bacterium found in humans and animals [ 12 ], in S. aureus, the gene activity that encodes penicillin-binding proteins causes a resistance reaction to beta-lactam antibiotics we know as MRSA [ 13 ], additionally resistant to non-beta-lactam medicines are MRSA strains. At first, MRSA was thought to cause nosocomial infections [ 14 ]. Nonetheless, MRSA colonization and infection have recently been found in a lot of people and animals who are not in clinical settings [ 15 ].…”

Section: Mrsa In Pet Animalsmentioning

confidence: 99%

Pet animals as reservoirs for spreading methicillin-resistant Staphylococcus aureus to human health

Khairullah¹,

Sudjarwo²,

Effendi³

et al. 2023

J Adv Vet Anim Res

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Methicillin-resistant Staphylococcus aureus (MRSA) is a strain of pathogenic bacteria that is a major problem in the world’s health. Due to their frequent interaction with humans, pets are one of the main risk factors for the spread of MRSA. The possibility for zoonotic transmission exists since frequently kept dogs and cats are prone to contract MRSA and act as reservoirs for spreading MRSA. The mouth, nose, and perineum are the primary locations of MRSA colonization, according to the findings of MRSA identification tests conducted on pets. The types of MRSA clones identified in cats and dogs correlated with MRSA clones infecting humans living in the same geographic area. A significant risk factor for the colonization or transmission of MRSA is human-pet contact. An essential step in preventing the spread of MRSA from humans to animals and from animals to humans is to keep hands, clothing, and floor surfaces clean.

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“…Although HA-MRSA and CA-MRSA strains may have different antimicrobial susceptibility patterns [ 15 ], both are now considered significant hazards to human health in the context of infectious diseases [ 16 ]. Understanding their respective distribution in the population is crucial for their treatment and management.…”

Section: Introductionmentioning

confidence: 99%

Detection of Genes Encoding Microbial Surface Component Recognizing Adhesive Matrix Molecules in Methicillin-Resistant Staphylococcus aureus Isolated from Pyoderma Patients

Alorabi

Ejaz

Khoso

et al. 2023

Genes

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Pyoderma is a common skin infection predominantly caused by Staphylococcus aureus. In addition to methicillin resistance, this pathogen is resistant to many other antibiotics, which ultimately limits the available treatment options. Therefore, the present study aimed to compare the antibiotic-resistance pattern, to detect the mecA gene and the genes encoding microbial surface component recognizing adhesive matrix molecules (MSCRAMMs) in S. aureus isolates. A total of 116 strains were isolated from patients suffering with pyoderma. Disk diffusion assay was opted to perform antimicrobial susceptibility testing of the isolates. Out of the isolates tested, 23–42.2% strains appeared susceptible to benzylpenicillin, cefoxitin, ciprofloxacin and erythromycin. While linezolid was found to be the most effective anti-staphylococcal drug, followed by rifampin, chloramphenicol, clindamycin, gentamicin and ceftaroline. Out of 116 isolates, 73 (62.93%) were methicillin-resistant S. aureus (MRSA). Statistically significant (p ≤ 0.05) differences in antibiotic resistance patterns between MRSA and methicillin-susceptible S. aureus (MSSA) were found. A significant association of resistance to ceftaroline, rifampin, tetracycline, ciprofloxacin, clindamycin, trimethoprim–sulfamethoxazole and chloramphenicol was found in MRSA. However, no significant difference was observed between MRSA and MSSA for resistance against gentamicin, erythromycin or linezolid. All cefoxitin-resistant S. aureus, nonetheless, were positive for the mecA gene. femA was found in all the MRSA isolates. Among other virulence markers, bbp and fnbB were found in all the isolates, while can (98.3%), clfA and fnbA (99.1%) were present predominately in MRSA. Thus, this study offers an understanding of antibiotic resistance MSCRAMMs, mecA, and femA gene patterns in locally isolated strains of S. aureus.

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Molecular Basis of Non-β-Lactam Antibiotics Resistance in Staphylococcus aureus

Cited by 26 publications

References 276 publications

Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Hospitalized Newborn Infants

Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Hospitalized Newborn Infants

Pet animals as reservoirs for spreading methicillin-resistant Staphylococcus aureus to human health

Detection of Genes Encoding Microbial Surface Component Recognizing Adhesive Matrix Molecules in Methicillin-Resistant Staphylococcus aureus Isolated from Pyoderma Patients

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