Molecular Basis of Pheromonogenesis Regulation in Moths

Hull, J. Joe; Fónagy, Adrien

doi:10.1007/978-3-030-05060-3_8

Cited by 6 publications

(7 citation statements)

References 288 publications

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“…Given the established role of PK2 peptides (e.g., PBANs) in lepidopteran pheromone biosynthesis [77,78], we sought to assess the potential in vivo pheromonotropic activity of synthetic LyghePKa and PKb in three species of moths, M. brassicae, O. nubilalis, and S. littoralis. Although slightly different species-specific protocols were used to examine pheromone blend titers, the general scheme utilized decapitated females that were maintained in Petri dishes with moist filter paper until treatment.…”

Section: Injection Of Synthetic Neuropeptides and Preparation Of Pheromone Gland Extractsmentioning

confidence: 99%

“…Given the well-established pheromonotropic role of PK2 peptides in lepidopterans [77,78], we next sought to determine if the Lygus peptides could stimulate pheromone biosynthesis in different moth species by injecting varying amounts of synthetic Ly-ghePKa and LyghePKb into decapitated females. Depending on geographical region, the M. brassicae sex pheromone blend consists of 2-3 components with the two predominant compounds, (Z)-11-hexadecenyl acetate (Z11-16:Ac) and hexadecyl acetate (16:Ac), present in a 93:7 ratio [79].…”

Section: In Vivo Pheromonotropic Activitymentioning

confidence: 99%

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Molecular and Functional Characterization of Pyrokinin-Like Peptides in the Western Tarnished Plant Bug Lygus hesperus (Hemiptera: Miridae)

Hull

Brent

Choi

et al. 2021

Insects

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The pyrokinin (PK) family of insect neuropeptides, characterized by C termini consisting of either WFGPRLamide (i.e., PK1) or FXPRLamide (i.e., PK2), are encoded on the capa and pk genes. Although implicated in diverse biological functions, characterization of PKs in hemipteran pests has been largely limited to genomic, transcriptomic, and/or peptidomic datasets. The Lygus hesperus (western tarnished plant bug) PK transcript encodes a prepropeptide predicted to yield three PK2 FXPRLamide-like peptides with C-terminal sequences characterized by FQPRSamide (LyghePKa), FAPRLamide (LyghePKb), and a non-amidated YSPRF. The transcript is expressed throughout L. hesperus development with greatest abundance in adult heads. PRXamide-like immunoreactivity, which recognizes both pk- and capa-derived peptides, is localized to cells in the cerebral ganglia, gnathal ganglia/suboesophageal ganglion, thoracic ganglia, and abdominal ganglia. Immunoreactivity in the abdominal ganglia is largely consistent with capa-derived peptide expression, whereas the atypical fourth pair of immunoreactive cells may reflect pk-based expression. In vitro activation of a PK receptor heterologously expressed in cultured insect cells was only observed in response to LyghePKb, while no effects were observed with LyghePKa. Similarly, in vivo pheromonotropic effects were only observed following LyghePKb injections. Comparison of PK2 prepropeptides from multiple hemipterans suggests mirid-specific diversification of the pk gene.

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Section: Injection Of Synthetic Neuropeptides and Preparation Of Pheromone Gland Extractsmentioning

confidence: 99%

Section: In Vivo Pheromonotropic Activitymentioning

confidence: 99%

Molecular and Functional Characterization of Pyrokinin-Like Peptides in the Western Tarnished Plant Bug Lygus hesperus (Hemiptera: Miridae)

Hull

Brent

Choi

et al. 2021

Insects

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“…Identifying the PG‐enriched expression of the desaturase gene can provide valuable insights into its role in pheromonogenesis. 41 We quantitatively measured the expression of all the six candidates using qRT‐PCR, and the rate of the desaturase expression between different ages (1–10 days) was compared. We observed a significantly higher expression level ( P < 0.001) of Ecau_D11 and a slightly lower expression of Ecau_D14 in the 1–2‐day‐old female E. cautella .…”

Section: Resultsmentioning

confidence: 99%

“…Identifying the PG-enriched expression of the desaturase gene can provide valuable insights into its role in pheromonogenesis. 41 We quantitatively measured the expression of all the six S1). candidates using qRT-PCR, and the rate of the desaturase expression between different ages (1-10 days) was compared.…”

Section: Relative Expression Level Of E Cautella Desatursesmentioning

confidence: 99%

Bioproduction of (Z,E)‐9,12‐tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast

Ding

Wang

Al-Saleh

et al. 2021

Pest Management Science

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BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae. RESULTS:We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses.CONCLUSION: The reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bioproduction technology for high-value storage pest control.

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“…PBAN receptor is a G protein‐coupled receptor located on the surface of PG cells (Rafaeli, 2009). When PBAN binds to its receptor, it triggers a secondary messenger, which in turn activates the corresponding enzymes and eventually leads to the biosynthesis of sex pheromones (Hull & Fónagy, 2019; Hull & Fónagy, 2020; Jurenka & Rafaeli, 2011; Matsumoto et al, 2010; Rafaeli, 2009). Usually, PBAN employs Ca 2+ and cAMP as secondary messengers to regulate sex pheromone biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Octopamine terminates sex pheromone biosynthesis by suppressing PBAN signal in moths

Yang

Zhang³

et al. 2022

Insect Molecular Biology

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The biosynthesis and termination of insect sex pheromones should be accurately regulated. In most moths, the biosynthesis and release of sex pheromones are regulated by a class of neuropeptides known as pheromone biosynthesis activating neuropeptides (PBANs). However, endogenous mechanisms underlying the termination of sex pheromone biosynthesis in moths remain elusive. In the present study, Helicoverpa armigera was employed as a model to investigate the role of octopamine (OA) in the inhibition of sex pheromone biosynthesis. Results demonstrated that the release of sex pheromones decreased with an increase in OA titres in older female moths. Moreover, OA treatment led to a significant decrease in sex pheromone production, female capability to attract male counterparts and subsequent female acceptance, indicating its inhibitory role in sex pheromone release. Subsequent qPCR and RNAi analyses revealed that OctβR was a key receptor of OA that regulated sex pheromone biosynthesis. In addition, the OA/OctβR signal suppressed intracellular Ca2+ levels and attenuated PBAN‐mediated increase in the enzyme activities of calcineurin and acetyl‐CoA carboxylase as demonstrated by OA treatment and OctβR‐RNAi. Altogether, these results revealed a mechanism underlying the inhibition of sex pheromone production by OA via suppression of PBAN signalling in moths.

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Molecular Basis of Pheromonogenesis Regulation in Moths

Cited by 6 publications

References 288 publications

Molecular and Functional Characterization of Pyrokinin-Like Peptides in the Western Tarnished Plant Bug Lygus hesperus (Hemiptera: Miridae)

Molecular and Functional Characterization of Pyrokinin-Like Peptides in the Western Tarnished Plant Bug Lygus hesperus (Hemiptera: Miridae)

Bioproduction of (Z,E)‐9,12‐tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast

Octopamine terminates sex pheromone biosynthesis by suppressing PBAN signal in moths

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