Molecular Basis of Resistance to Selected Antimicrobial Agents in the Emerging Zoonotic Pathogen Streptococcus suis

Gurung, Mamata; Tamang, Migma Dorji; Moon, Dong Chan; Kim, Su-Ran; Jeong, Jin-Ha; Jang, Geum-Chan; Jung, Suk-Chan; Park, Yong‐Ho; Lim, Suk-Kyung

doi:10.1128/jcm.00123-15

Cited by 47 publications

(60 citation statements)

References 37 publications

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“…A proportionately higher susceptibility to β -lactams and aminoglycosides were also reported previously [5,11,17,22]. On the contrary, high levels of resistance to these agents were also reported by various workers [9,19,23-25]. …”

Section: Discussionsupporting

confidence: 70%

Prevalence of multiple drug resistant Streptococcus suis in and around Guwahati, India

Devi¹,

Dutta²,

Rajkhowa

et al. 2017

Vet World

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Aim:This study was conducted to determine the prevalence and antimicrobial susceptibility of Streptococcus suis and their resistance patterns isolated from both clinically healthy carriers and diseased pigs in and around Guwahati, Assam, India.Materials and Methods:A total of 497 samples were collected during October, 2012, to April, 2014, from clinically healthy (n=67) and diseased (n=230) pigs of varying age and either sex maintained under organized and unorganized farming systems. Samples were processed for isolation and identification of S. suis by biochemical characterization and polymerase chain reaction targeting the housekeeping gene glutamate dehydrogenase. In vitro antimicrobial susceptibility of the recovered isolates against nine antibiotic groups comprising 17 antimicrobial agents was studied by standard method.Results:Of the 497 samples examined, 7 (1.41%) isolates were confirmed to be S. suis of which 5 (1.87%) and 2 (0.87%) were derived from clinically healthy and diseased pigs, respectively. All the isolates were susceptible to gentamicin, amikacin, and erythromycin (100%) followed by the penicillin group and enrofloxacin (85.71%), ceftriaxone, doxycycline HCL, ofloxacin and chloramphenicol (71.43%), to kanamycin, clindamycin and co-trimoxazole (42.85%). The isolates showed least susceptibility to cefalexin, tetracycline and streptomycin (28.57%). All the five S. suis isolates from clinically healthy pigs were susceptible to penicillin G, amoxyclav, doxycycline HCl, gentamicin, amikacin and erythromycin, 80.00% isolates susceptible to ampicillin, enrofloxacin and ofloxacin, 60.00% to ceftriaxone, kanamycin and chloramphenicol, 40% to cefalexin, tetracycline, clindamycin and co-trimoxazole, respectively. Only 20.00% isolates were susceptible to streptomycin. Both the isolates recovered from diseased pigs were susceptible to ampicillin, ceftriaxone, gentamicin, amikacin, enrofloxacin, erythromycin, and clindamycin. On the other hand, both the isolates were resistant to cefalexin, tetracycline, doxycycline HCL, and kanamycin. Altogether five different resistance patterns (multi-drug resistance) were observed. Of the seven S. suis isolates, two isolates were susceptible to all the 17 antimicrobial agents, one isolate was resistant to four antimicrobial agents, two isolates to seven agents, one isolate to nine agents, and one isolate exhibited resistance to 14 antimicrobial agents.Conclusion:This study was conducted to determine the prevalence of S. suis in clinically healthy and diseased pigs and their antimicrobial susceptibility patterns. All the isolates were susceptible to gentamicin, amikacin and erythromycin, and most of them were resistant to cefalexin, tetracycline and streptomycin. Five different patterns of antimicrobial resistance (multi-drug resistance) were observed.

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Section: Discussionsupporting

confidence: 70%

Prevalence of multiple drug resistant Streptococcus suis in and around Guwahati, India

Devi¹,

Dutta²,

Rajkhowa

et al. 2017

Vet World

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“…Among the 59 tet genes, the tet(O) gene encoding a ribosome protective protein (RPP) appears to be the most common determinant of resistance in S. suis strains isolated from both pigs and humans globally [2,5,17,23,26]. On the other hand, although the tet(M) gene (also encoding a RPP) was detected only in 2.0% and 3.9% of Korean [5] and Italian [17] S. suis pig strains, respectively, it was detected in 36.4% of human strains in Hong Kong [2]. The tet(M) gene was the most commonly detected TC resistance gene in Enterococcus faecalis isolated from swine feces (20/22) [12].…”

mentioning

confidence: 99%

“…None of the strains were resistant to β-lactams. PCG resistance in S. suis was first reported in the UK in a serotype 2 strain isolated from a human patient in 1980 [18], and has since emerged in S. suis isolated from pigs globally [5,6,28]. However, China [28] and the UK [6] isolates exhibit low resistance rates (9.1% and 5%, respectively), and resistant strains have not been detected in Vietnam [7].…”

mentioning

confidence: 99%

Changes in antimicrobial resistance phenotypes and genotypes in <i>Streptococcus suis</i> strains isolated from pigs in the Tokai area of Japan

Ichikawa¹,

Oshima²,

Yamagishi³

et al. 2020

The Journal of Veterinary Medical Science

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Streptococcus suis strains isolated from porcine endocarditis and tonsils in the Tokai area of Japan during 2004-2007 and 2014-2016 (n=114) were tested for antimicrobial susceptibility and distribution of selected resistance genes. No strains showed resistance to penicillin, ampicillin, cefotaxime, meropenem, vancomycin, and levofloxacin. High resistance to tetracycline (80.7%), clindamycin (65.8%), erythromycin (56.1%), and clarithromycin (56.1%) was observed. In chloramphenicol and sulfamethoxazole-trimethoprim, there was a trend towards increased resistance between the first (2004)(2005)(2006)(2007) and second (2014)(2015)(2016) periods. tet(O) and erm(B) genes were the most frequently detected, and tet(M) and mef(A/E) genes were only detected in strains isolated during 2014-2016. These results indicate that chloramphenicol and sulfamethoxazoletrimethoprim resistance, and tet(M) and mef(A/E) genes emerged in S. suis of this area after 2014.

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“…Previous reports suggested that S. suis strains had a high resistance to macrolides, lincosamides, tetracycline, and sulfonamides [11][12][13][14][15] . The S. suis isolates in this study showed a high resistance to macrolides, lincosamides, tetracycline, and sulfonamides, as well as a high resistance to quinolones and aminoglycosides.…”

Section: Discussionmentioning

confidence: 98%

“…Antimicrobial agents play an important role in the treatment and control of S. suis infection. However, irrational use of antibiotics leads to a continual increase in drug resistance of S. suis worldwide [11][12][13][14][15] . Therefore, antimicrobial susceptibility testing is a crucial step preceding the prescription of antibiotic treatment.…”

Section: Introductionmentioning

confidence: 99%

Güney Çin’de Hastalıklı ve Sağlıklı Domuzlarda Streptococcus suis’in Antimikrobiyal Direnci, Serotipleri ve Genetik Çeşitliliği

Peng¹,

ChongBo²,

Yang³

et al. 2019

Kafkas Univ Vet Fak Derg

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In the study, the characteristics of 81 Streptococcus suis isolates recovered from healthy and diseased pigs in Southern China were analyzed. These isolates showed differential resistance rates of over 40% to 22 and over 80% to 11 antibiotics out of 42 tested drugs. These isolates belonged to 6 serotypes (2, 3, 7, 9, 19, and 28), 9.9% of which were characterised as non-typable (NT), and were classified into 15 sequence types (STs), 8 of which were new (ST812-ST815, ST821-823, and ST832). Additionally, 6 virulence-associated gene patterns (VAGPs) and 10 random amplified polymorphic DNA (RAPD) patterns were found among the 81 strains. Among the 4 genotyping methods used in this study, multilocus sequence typing (MLST) provided the highest resolution for identification of S. suis strains. Significant differences in STs, RAPD patterns, and VAGPs were observed between strains isolated from healthy pigs and those isolated from diseased pigs. This study did not find any statistically significant correlations between antimicrobial resistance and serotypes, STs, RAPD patterns, VAGPs, or strain sources. Serotype 2 strains were predominant (44.4%) in the study and all of these strains showed the molecular characteristics of highly pathogenic strains in VAGP, GDH sequence types, and MLST. Our results showed that S. suis strains in Southern China are continually evolving, and therefore increased surveillance of S. suis in piggeries must be considered. Güney Çin'de Hastalıklı ve Sağlıklı Domuzlarda Streptococcus suis'in Antimikrobiyal Direnci, Serotipleri ve Genetik Çeşitliliği ÖzBu çalışmada Güney Çin'de sağlıklı ve hasta domuzlardan elde edilen 81 Streptococcus suis izolatının özellikleri incelendi. İzolatlar, test edilen 42 antibiyotikten 22'sine %40'ın üzerinde ve 11'ine %80'in üzerinde değişen direnç gösterdi. Bu izolatlar 6 serotipe (2,3,7,9,19 ve 28) ait olup %9.9'u tiplendirilemeyen olarak karakterize edildi ve 8'i yeni (ST812-ST815, ST821-823 ve ST832) olmak üzere 15 sekans tipine (ST) sınıflandırıldı. Ayrıca, 81 suşta 6 adet virulans ile ilişkili gen şeması (VAGP) ve 10 adet rastgele amplifiye polimorfik DNA (RAPD) şeması bulundu. Çalışmada kullanılan 4 genotiplendirme metodu arasında multilokus sekans tiplendirme (MLST) S. suis suşlarının tespitinde en yüksek rezolusyonu sağladı. Sağlıklı ve hasta domuzlardan izole edilen suşlar arasında sekans tiplerinde, RAPD şemasında ve VAGP'lerde anlamlı farklılıklar gözlemlendi. Çalışmada antimikrobiyal direnç ile serotipler, ST, RAPD şeması, VAGP'ler veya suş kaynakları arasında istatistiksel olarak bir ilişki tespit edilmedi. Serotip 2 suşu çalışmadaki predominant olandı ve tüm bu suşlar VAGP, GDH sekans tipi ve MIST'de oldukça patojenik suşların moleküler özelliklerini göstermekteydi. Elde edilen sonuçlar Güney Çin'de S. suis suşlarının sürekli olarak değiştiğini ve bu yüzden domuzlarda S.suis takibinin gerekli olduğunu göstermiştir.

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Molecular Basis of Resistance to Selected Antimicrobial Agents in the Emerging Zoonotic Pathogen Streptococcus suis

Cited by 47 publications

References 37 publications

Prevalence of multiple drug resistant Streptococcus suis in and around Guwahati, India

Prevalence of multiple drug resistant Streptococcus suis in and around Guwahati, India

Changes in antimicrobial resistance phenotypes and genotypes in <i>Streptococcus suis</i> strains isolated from pigs in the Tokai area of Japan

Güney Çin’de Hastalıklı ve Sağlıklı Domuzlarda Streptococcus suis’in Antimikrobiyal Direnci, Serotipleri ve Genetik Çeşitliliği

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