Molecular basis of somatosensation in insects

Liu, Chenxi; Zhang, Wei

doi:10.1016/j.conb.2022.102592

Cited by 4 publications

(3 citation statements)

References 86 publications

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“…For reproduction‐related genes, we identified spire, vitellogenins (Vgs), selenoprotein F (SPFs), Tudor, doublesex (Dmrt), sex peptide receptors (SPs), Soxes, and vasa (Gustafson & Wessel, 2010; Li et al., 2020; Manseau & Schüpbach, 1989; Poels et al., 2010; Wilson & Dearden, 2008). In terms of temperature adaptation‐related genes, we identified Trehalases (Trets), heat shock proteins (HSP), DnaJ, and transient receptor potential (TRPs) (Chen et al., 2021; Liu & Zhang, 2022; Tang et al., 2018). Last, for winged morph differentiation‐related genes, we identified six wingless (Wnt), ecdysone receptors (ECRs), ecdysone‐inducible protein E75, insulin receptor (InsR), insulin‐like growth factor‐binding proteins (IGFBPs), insulin‐like peptide (ILP), juvenile hormone esterase (JHE), juvenile hormone‐binding proteins (JHBPs), protein germ cell‐less (CGL), methoprene‐tolerant (Met), and epidermal growth factor receptor (EGFR) (Corona et al., 2016; Hayes et al., 2019; Llimargas & Lawrence, 2001; Truman, 2019).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome analysis and identification of genes related to environmental adaptation of Grylloprimevala jilina Zhou & Ren 2023

Zhou,

et al. 2023

Ecology and Evolution

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Grylloprimevala jilina is a true cave insect living in the dark areas of caves. It has the characteristics of sparse skin pigmentation, degeneration of the compound eyes and monocular eyes, and obvious preference for high‐humidity and low‐temperature environments. Given the highly specialized, rare, and limited distribution, G. jilina is considered an endangered species and also a first‐level national protected insect in China. Cave creatures often undergo dramatic morphological changes in their sensory systems to adapt to the cave environment. Most previous studies mainly focused on morphological adaptive changes in cave insects, and only a few studied the changes at the gene level. In this study, we performed transcriptome analysis of G. jilina and constructed phylogenetic trees of genes that are related to environmental adaptation, including chemosensory, visual‐related, reproduction‐related, temperature adaptation‐related, and winged morph differentiation‐related genes. Besides, the expression levels of environmental adaption‐related genes in different tissues, including antennae, heads, thoraxes, abdomens, legs, and tails, were analyzed. The results showed the loss of chemosensory genes and vision‐related genes, the conservation of reproduction‐related genes and temperature adaptation‐related genes, and the conservation of wing‐related genes despite the loss of wings, and the results were consistent with other cave insects. The identification and expression study of genes possibly related to the environmental adaptability in G. jilina provided basic data for the protection of this endangered species and increased knowledge about insect evolution in general.

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Section: Methodsmentioning

confidence: 99%

Transcriptome analysis and identification of genes related to environmental adaptation of Grylloprimevala jilina Zhou & Ren 2023

Zhou,

et al. 2023

Ecology and Evolution

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“…This temperature preference or thermotaxis has been used as an index to study thermosensory functions in model organisms like Drosophila melanogaster (fruit fly) and Caenorhabditis elegans (nematodes) ( Angilletta et al, 2002 ). In insects, and particularly in Drosophila, various genes related to temperature sensation, including TRP channels, have been identified ( Montell, 2021 ; Liu and Zhang, 2022 ) ( Figure 1 ). Moreover, mechanisms for regulating body temperature through behavioral thermoregulation have been described.…”

Section: Thermosensation In a Drosophila Modelmentioning

confidence: 99%

Functional relationship between peripheral thermosensation and behavioral thermoregulation

Suito,

Tominaga

2024

Front. Neural Circuits

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Thermoregulation is a fundamental mechanism for maintaining homeostasis in living organisms because temperature affects essentially all biochemical and physiological processes. Effector responses to internal and external temperature cues are critical for achieving effective thermoregulation by controlling heat production and dissipation. Thermoregulation can be classified as physiological, which is observed primarily in higher organisms (homeotherms), and behavioral, which manifests as crucial physiological functions that are conserved across many species. Neuronal pathways for physiological thermoregulation are well-characterized, but those associated with behavioral regulation remain unclear. Thermoreceptors, including Transient Receptor Potential (TRP) channels, play pivotal roles in thermoregulation. Mammals have 11 thermosensitive TRP channels, the functions for which have been elucidated through behavioral studies using knockout mice. Behavioral thermoregulation is also observed in ectotherms such as the fruit fly, Drosophila melanogaster. Studies of Drosophila thermoregulation helped elucidate significant roles for thermoreceptors as well as regulatory actions of membrane lipids in modulating the activity of both thermosensitive TRP channels and thermoregulation. This review provides an overview of thermosensitive TRP channel functions in behavioral thermoregulation based on results of studies involving mice or Drosophila melanogaster.

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“…Iris Salecker, INSERM U1024 Institut de biologie de l'Ecole Normale Supérieure, France melanogaster PNS comprises thousands of sensory organs located in stereotyped positions throughout the body. The most abundant sensory organs are mechanosensory bristles (Held, 1991;Liu and Zhang, 2022), tactile hairs covering most of the adult epidermis (Figure 1A). All tactile bristle cells originate from the asymmetric cell division of a single precursor cell, the sensory organ precursor (SOP), which itself is selected by lateral inhibition within the epidermal layer (Gho et al, 1996;Lai and Orgogozo, 2004).…”

Section: Open Access Edited Bymentioning

confidence: 99%

Photoablation at single cell resolution and its application in the Drosophila epidermis and peripheral nervous system

Mangione

D’Antuono²,

Tapon³

2023

Front. Physiol.

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Tissues contain diverse cell populations that, together, make up physiologically functional units. A remarkable example is the animal epidermis, where neuronal and non-neuronal cells intermingle to allow somatosensory perception. In the peripheral nervous system (PNS), the tight association between heterogenous cell types poses challenges when the structural and physiological contributions of neuronal and surrounding cells need to be dissected with suitable precision. When genetic tools for cell-specific, spatiotemporally controlled gene expression are not available, targeted cell ablation represents a considerable obstacle. Here, we describe an efficient method to overcome this limitation and demonstrate its application to the study of the differentiating Drosophila epidermis and PNS. This methodology relies on the use of near infrared (NIR) femtosecond (fs) laser pulses for ablation of the desired cells at the desired time. We show how to confine the photodamage to the targeted cell to induce its death, without harming neighbouring tissues or structures. We validated our approach in the Drosophila PNS by studying the responses of photo-ablated neurons, non-neuronal cells, and the surrounding epidermis. Diverse cellular behaviours including cell extrusion, cell rearrangements and cell shape changes can be monitored in vivo immediately after damage, as well as for several hours post-ablation with high optical resolution using confocal microscopy. This methodology provides a flexible tool to ablate individual cells with high precision and study morphological responses to cell loss in targeted areas or neighbouring structures. We anticipate that this protocol can be easily adapted to other model systems and tissues.

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Molecular basis of somatosensation in insects

Cited by 4 publications

References 86 publications

Transcriptome analysis and identification of genes related to environmental adaptation of Grylloprimevala jilina Zhou & Ren 2023

Transcriptome analysis and identification of genes related to environmental adaptation of Grylloprimevala jilina Zhou & Ren 2023

Functional relationship between peripheral thermosensation and behavioral thermoregulation

Photoablation at single cell resolution and its application in the Drosophila epidermis and peripheral nervous system

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