Molecular basis underlying male sterility in bHLH142 overexpressing rice

Ko, Swee-Suak; Li, Min-Jeng; Lin, Yi-Chun; Hsing, Hong-Xian; Yang, Tingting; Chen, Tien-Kuan; Jhong, Chung-Min; Ku, Maurice S. B.

doi:10.1101/116996

Cited by 3 publications

(3 citation statements)

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“…1A). Moreover, overexpressing bHLH142 caused premature upregulation of EAT1 (Ko et al, 2017). Third, TDR interacts with EAT1 (Ji et al, 2013;Ko et al, 2014;Niu et al, 2013) in regulating the expression of the downstream genes for pollen maturation.…”

Section: Discussionmentioning

confidence: 98%

“…In addition, we have also shown that bHLH142 interacts with TDR to form a heterodimer and then co-modulate the expression of EAT1 expression (Ko et al, 2014). Moreover, overexpression of homologous bHLH142 leads to premature upregulation of EAT1 transcription and causes early tapetal PCD, suggesting that timely and precise control of bHLH142 level is critical to normal rice pollen development (Ko et al, 2017).…”

Section: Introductionmentioning

confidence: 87%

“…The tapetum layer is the innermost layer of the anther wall that provides nutrients for the development of microspores (Goldberg et al, 1993). Timely control of tapetal program cell death (PCD) is crucial for pollen maturation (Ko et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Rice transcription factor GAMYB modulates bHLH142 and is homeostatically regulated by TDR during anther tapetal and pollen development

et al. 2021

Journal of Experimental Botany

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GAMYB, UDT1, TIP2/bHLH142, TDR, and EAT1/DTD are important transcription factors (TFs) that play a crucial role during rice pollen development. This study demonstrates that bHLH142 acts downstream of UDT1 and GAMYB and works as a “hub” in these two pollen pathways. We show that GAMYB modulates bHLH142 expression through specific binding to the MYB motif of bHLH142 promoter during early stage of pollen development; while TDR acts as a transcriptional repressor of the GAMYB modulation of bHLH142 by binding to the E-box close to the MYB motif on the promoter. The altered expression of TFs highlights the importance that a tight, precise, and coordinated regulation among these TFs is essential for normal pollen development. Most notably, this study illustrates the regulatory pathways of GAMYB and UDT1 that rely on bHLH142 in a direct and an indirect manner, respectively, and function in different tissues with distinct biological functions during pollen development. This study advances our understanding of the molecular mechanisms of rice pollen development.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 87%

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Rice transcription factor GAMYB modulates bHLH142 and is homeostatically regulated by TDR during anther tapetal and pollen development

et al. 2021

Journal of Experimental Botany

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GAMYB modulatesbHLH142and is homeostatically regulated by TDR during rice anther tapetal and pollen development

et al. 2020

Preprint

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GAMYB, UDT1, TIP2/bHLH142, TDR, and EAT1/DTD are important transcription factors (TFs) that play a crucial role during rice pollen development. This study demonstrates that bHLH142 acts downstream of UDT1 and GAMYB and works as a “hub” in these two pollen pathways. We show that GAMYB modulates bHLH142 expression through specific binding to the MYB motif of bHLH142 promoter during early stage of pollen development; while TDR acts as a transcriptional repressor of the GAMYB modulation of bHLH142 by binding to the E-box close to the MYB motif on the promoter. The up- and down-regulation of TFs highlights the importance that a tight, precise, and coordinated regulation among these TFs is essential for normal pollen development. Most notably, this study illustrates the regulatory pathways of GAMYB and UDT1 that rely on bHLH142 in a direct and an indirect manner, respectively, and function in different tissues with distinct biological functions during pollen development. This study advances our understanding of the molecular mechanisms of rice pollen development.HighlightGAMYB can directly modulate the transactivation of the bHLH142, but the modulation is repressed by TDR to keep the homeostasis of bHLH142 gene expression to ensure normal pollen development.

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Comparative Transcriptome Analysis between Fertile and CMS Flower Buds in Wucai (Brassica campestris L.)

Chen

Zhang

et al. 2018

BMC Genomics

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BackgroundWucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen) is a variant of nonheading Chinese cabbage (Brassica campestris L.), which is one of the major vegetables in China. Cytoplasmic male sterility (CMS) has been used for Wucai breeding in recent years. However, the underlying molecular mechanism of Wucai CMS remains unclear. In this study, the phenotypic and cytological features of Wucai CMS were observed by anatomical analysis, and a comparative transcriptome analysis was carried out to identify genes related to male sterility using Illumina RNA sequencing technology (RNA-Seq).ResultsMicroscopic observation demonstrated that tapetum development was abnormal in the CMS line, which failed to produce fertile pollen. Bioinformatics analysis detected 4430 differentially expressed genes (DEGs) between the fertile and sterile flower buds. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to better understand the functions of these DEGs. Among the DEGs, 35 genes (53 DEGS) were implicated in anther and pollen development, and 11 genes were involved in pollen cell wall formation and modification; most of these showed downregulated expression in sterile buds. In addition, several genes related to tapetum development (A6, AMS, MS1, MYB39, and TSM1) and a few genes annotated to flowering (CO, AP3, VIN3, FLC, FT, and AGL) were detected and confirmed by qRT-PCR as being expressed at the meiosis, tetrad, and uninucleate microspore stages, thus implying possible roles in specifying or determining the fate and development of the tapetum, male gametophyte and stamen. Moreover, the top four largest transcription factor families (MYB, bHLH, NAC and WRKY) were analyzed, and most showed reduced expression in sterile buds. These differentially expressed transcription factors might result in abortion of pollen development in Wucai.ConclusionThe present comparative transcriptome analysis suggested that many key genes and transcription factors involved in anther development show reduced gene expression patterns in the CMS line, which might contribute to male sterility in Wucai. This study provides valuable information for a better understanding of CMS molecular mechanisms and functional genome studies in Wucai.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5331-4) contains supplementary material, which is available to authorized users.

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Molecular basis underlying male sterility in bHLH142 overexpressing rice

Cited by 3 publications

References 45 publications

Rice transcription factor GAMYB modulates bHLH142 and is homeostatically regulated by TDR during anther tapetal and pollen development

Rice transcription factor GAMYB modulates bHLH142 and is homeostatically regulated by TDR during anther tapetal and pollen development

GAMYB modulatesbHLH142and is homeostatically regulated by TDR during rice anther tapetal and pollen development

Comparative Transcriptome Analysis between Fertile and CMS Flower Buds in Wucai (Brassica campestris L.)

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