Molecular beacons immobilized within suspended core optical fiber for specific DNA detection

Nguyen, Linh V.; Warren‐Smith, Stephen C.; Cooper, Alan; Monro, Tanya M.

doi:10.1364/oe.20.029378

Cited by 33 publications

(21 citation statements)

References 22 publications

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“…However, to facilitate interaction of the light propagating in the fiber core with the analyte for use in biosensing, chemical etching [13] or tapering [14] typically has to be carried out on the fibers, leading to inferior mechanical properties. More advanced designs of optical fibers such as photonic crystal fiber [15] or suspended core microstructured fiber [12,16] can allow direct interaction of the light in the fiber core with the analyte solution flowing through the air-holes running along the fiber core. However, it is difficult to realize an all-fiber configuration in such cases because the air-holes need to be open to allow the flow of the solution through these holes.…”

Section: Introductionmentioning

confidence: 99%

Interferometric-type optical biosensor based on exposed core microstructured optical fiber

Nguyen

Hill

Warren‐Smith

et al. 2015

Sensors and Actuators B: Chemical

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In all cases accepted manuscripts should:  link to the formal publication via its DOI  bear a CC-BY-NC-ND license -this is easy to do, click here to find out how  if aggregated with other manuscripts, for example in a repository or other site, be shared in alignment with our hosting policy  not be added to or enhanced in any way to appear more like, or to substitute for, the published journal article Abstract: This work presents a novel biosensor using the multimode interference effect in an exposed core microstructured optical fiber (ECF). In this work biotin molecules are immobilized onto the ECF core surface to serve as the capturing probe for streptavidin, the target molecules.Since each distinct guided mode in the ECF interacts with the surrounding medium differently, the interference between any two specific modes will experience a fringe shift (or phase change) upon a change in the refractive index (RI) of the surrounding medium, or a localized RI change on the surface of the ECF core as a result of a biological binding event. In our experiment, the interferometric sensing platform was realized by splicing a section of ECF with lead-in and leadout single mode fibers (SMFs). An interference pattern is obtained in the transmission spectrum as the result of multiple excited modes (excited and re-collected at the lead-in and lead-out splicing points) propagating in the ECF with different propagation constants. The interference pattern is non-uniform, indicating that there are more than two modes involved. Fast Fourier transform (FFT) is used to separate individual interference patterns that contribute to this complex spectrum and monitor their phase changes upon RI variation of the surrounding medium. In this way multiple RI sensitivities can be realized because each spatial frequency possesses a distinct sensitivity with respect to the surrounding RI. The operation of this device was validated by measuring the phase changes that occur when the sensing platform was subjected to solutions of different RIs or functionalized with different molecules. A biosensor was demonstrated based on this novel platform using biotin as the capturing probe to detect streptavidin with low non-specific adsorption. The proposed platform is reliable, cost-effective, and offers a potential label-free biosensing alternative to the widely used surface plasmon resonance (SPR) technique.

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Section: Introductionmentioning

confidence: 99%

Interferometric-type optical biosensor based on exposed core microstructured optical fiber

Nguyen

Hill

Warren‐Smith

et al. 2015

Sensors and Actuators B: Chemical

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“…The immobilization of two types of MBs on the surface of the SCF core was carried out following the procedure detailed in our previous publication [18] using a combination of the fuzzy nanoassembly technique [20] and the biotin-streptavidin binding mechanism [21]. An in-house made pump system was used to flow the required solutions through the SCF air-holes for deposition of different immobilization layers.…”

Section: Methodsmentioning

confidence: 99%

“…Among such optical fiber designs for biochemical sensing purposes, the development of a special type of optical fiber named “suspended core microstructured optical fiber”, which can provide strong interactions between the guided light in the fiber core and samples loaded within the fiber voids in addition to simple filling characteristics, while being simple to fabricate has been proposed and developed in our group [14]. The SCF has been demonstrated for a variety of biochemical sensing applications based on fluorescence measurements such as selective detection of biomolecules [15], chemicals [16], and real-time distributed measurements using exposed-core SCF [17] or specific DNA sensing in a dip-sensing fashion [18]. SCFs are hollow fibers with a small (micron-scale) solid core supported by a few thin struts (3 or more struts depending on the design) reflecting the name “suspended core fiber”.…”

Section: Introductionmentioning

confidence: 99%

Genotyping Single Nucleotide Polymorphisms Using Different Molecular Beacon Multiplexed within a Suspended Core Optical Fiber

Nguyen

Giannetti

Warren‐Smith

et al. 2014

Sensors

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We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild- or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF. The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild- and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons.

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“…This is unlike other surface functionalization process such as those based on thiols or silanes, which only work on silver or gold, or require substantial preparation of the substrate in order to create the required hydroxyl groups. Therefore, PE coatings can be deposited on metallic [105,106], polymer [107,108], and glass surfaces [109,110], and even on living organisms [111] which highlights their biocompatibility. PE coatings are also highly reproducible with the thickness of individual layers being only dictated by both the ionic strength of the buffer in which the PE are dissolved (usually NaCl solutions) as well as the concentration of the PE in solution [112].…”

Section: Physisorptionmentioning

confidence: 99%

Whispering Gallery Mode Devices for Sensing and Biosensing

François¹,

Zhi

Meldrum

2015

Photonic Materials for Sensing, Biosensing and Display Devices

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Optical sensors based on resonant whispering gallery mode (WGM) optical cavities are discussed in the context of recent research developments. WGM-based sensors are among the most sensitive optical sensing devices currently known, especially when combined with surface plasmon effects. The basic theory of WGM "reactive" sensing is first discussed, along with the basic parameters that are used to characterize such devices (i.e., sensitivity, signal-to-noise ratio, and detection limit). Surface chemistry methods are of critical importance for targeted sensing applications and the fundamentals of this aspect are examined. The performance of fluorescent WGM-based devices is compared to state-of-the-art "evanescently-coupled" structures. Finally, the performance of WGM-based optical sensors is compared and contrasted to a range of alternative optical microsensor technologies currently under development.A. François

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Molecular beacons immobilized within suspended core optical fiber for specific DNA detection

Cited by 33 publications

References 22 publications

Interferometric-type optical biosensor based on exposed core microstructured optical fiber

Interferometric-type optical biosensor based on exposed core microstructured optical fiber

Genotyping Single Nucleotide Polymorphisms Using Different Molecular Beacon Multiplexed within a Suspended Core Optical Fiber

Whispering Gallery Mode Devices for Sensing and Biosensing

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