Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae)

Sanches, Gustavo Seron; Évora, Patricia Martinez; Mangold, Atilio José; Jittapalapong, Sattaporn; Rodríguez-Mallón, Alina; Guzmán, Pedro E. Encinosa; Bechara, Gervásio Henrique; Camargo‐Mathias, Maria Izabel

doi:10.1016/j.vetpar.2015.11.007

Cited by 46 publications

(33 citation statements)

References 27 publications

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“…in the Neotropical region: tropical species (occurring from northern Mexico, Central America, and tropical areas of South America) and temperate species (found temperate and cold localities from the southern cone of South America) [5–7]. This notion is supported by more recent studies [8–10], indicating that ticks currently identified as “ R. sanguineus ” actually belong to at least two distinct species.…”

Section: Discussionmentioning

confidence: 85%

Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal

et al. 2017

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BackgroundThe taxonomic status of the brown dog tick Rhipicephalus sanguineus (sensu stricto) is a subject of on-going debate; there is a consensus that populations of this tick species should be referred to as R. sanguineus (sensu lato) until its taxonomic status is resolved. Recent genetic studies revealed the existence of more than one lineage of R. sanguineus (s.l.) in temperate countries. In this study, we assessed the genetic identity of ticks collected from rural dogs living in several areas located in all major geographical regions of Portugal.MethodsA total of 347 ticks were collected from rural dogs living in different regions of Portugal. These ticks were morphologically identified and partial mitochondrial 16S rRNA gene sequences (~300 bp) were obtained from representative specimens.ResultsThe ticks were morphologically identified as Ixodes ricinus (seven males and 27 females), Rhipicephalus bursa (one male), Rhipicephalus pusillus (one female) and R. sanguineus (s.l.) (two larvae, 101 nymphs, 108 males and 100 females). Partial mitochondrial 16S rRNA gene sequences were obtained from 58 R. sanguineus (s.l.) specimens, and all of them were genetically identified as belonging to the so-called temperate lineage of R. sanguineus (s.l.)ConclusionsThese results strongly suggest that the temperate species of R. sanguineus (s.l.) is the only representative of this tick group found on dogs in Portugal. It also adds weight to the hypothesis that Rhipicephalus turanicus is not present in this country, although further investigations are necessary to confirm this.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2072-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 85%

Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal

et al. 2017

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“…[92], Sanches et al . [93] who divided the R. sanguineus group into tropical and temperate strains. On the other hand, the phylogenetic tree of 18S rDNA gene sequence (KU198407), placed the Egyptian brown dog tick in a separate clad that may return to 18S rRNA identified at genera level (KF958435.1) [51], subsequently, doubted the usefulness and applicability of such gene in molecular taxonomy of ticks (Figure-7a).…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt

Abdullah¹,

El-Molla²,

Salib³

et al. 2016

Vet World

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Aim:Rickettsioses have an epidemiological importance that includes pathogens, vectors, and hosts. The dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii play important roles as vectors and reservoirs of Rickettsiae. The aim of this study was to determine the prevalence of Rickettsiae in ixodid ticks species infesting dogs and camels in Egypt, in addition to, the morphological and molecular identification of R. sanguineus and H. dromedarii.Materials and Methods:A total of 601 and 104 of ticks’ specimens were collected from dogs and camels, respectively, in Cairo, Giza and Sinai provinces. Hemolymph staining technique and OmpA and gltA genes amplification were performed to estimate the prevalence rate of Rickettsiae in ticks. For morphological identification of tick species, light microscope (LM) and scanning electron microscope (SEM) were used. In addition to the phylogenetic analyses of 18S rDNA, Second internal transcript spacer, 12S rDNA, cytochrome c oxidase subunit-1, and 16S rDNA were performed for molecular identification of two tick species.Results:The prevalence rate of Rickettsiae in ticks was 11.6% using hemolymph staining technique and 6.17% by OmpA and gltA genes amplification. Morphological identification revealed that 100% of dogs were infested by R. sanguineus while 91.9% of camels had been infested by H. dromedarii. The phylogenetic analyses of five DNA markers confirmed morphological identification by LM and SEM. The two tick species sequences analyses proved 96-100% sequences identities when compared with the reference data in Genbank records.Conclusion:The present studies confirm the suitability of mitochondrial DNA markers for reliable identification of ticks at both intra- and inter-species level over the nuclear ones. In addition to, the detection of Rickettsiae in both ticks’ species and establishment of the phylogenetic status of R. sanguineus and H. dromedarii would be useful in understanding the epidemiology of ticks and tick borne rickettsioses in Egypt.

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“…The specific taxonomic classification of R. sanguineus sensu lato remains subject to ongoing debate (Dantas‐Torres, ; Moraes‐Filho et al ., ; Nava et al ., , ; Dantas‐Torres et al ., ; Dantas‐Torres & Otranto, ; Sanches et al ., ). According to Nava et al .…”

Section: Introductionmentioning

confidence: 97%

“…Other authors have supported and extended these findings by suggesting the existence of at least two climate‐related lineages based on climate variables correlation analysis and including a ‘tropical lineage’ and a ‘temperate lineage’ (Szabó et al ., ; Moraes‐Filho et al ., ; Sanches et al ., ; Zemtsova et al ., ). Recently, (Dantas‐Torres et al ., ) highlighted four genetically distinct morphotypes in R. sanguineus s.l ., designated as Type I (from Greece), Type II (from Italy, France, Spain and Portugal), Type III (from India) and R. sanguineus s.l .…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA analysis of Rhipicephalus sanguineus s.l. from the western Iberian peninsula

Almeida

Simões

Coimbra-Dores

et al. 2017

Medical Vet Entomology

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Rhipicephalus sanguineus Latreille (1806) (Ixodida: Ixodidae) is considered to be the most widely distributed tick and to have a vast range of habitats and hosts, including livestock, pets and wildlife. In addition to morphological differences, recent investigations using approaches based on molecular genetic markers have revealed the existence of different R. sanguineus lineages in different geographic regions. In this study, 475 ticks collected from dogs in the western Iberian peninsula were studied both morphologically and genetically, using 12S and 16S rDNA and COI gene markers in order to clarify the controversy over the systematic status of R. sanguineus sensu lato in Western Europe, and to compare the present data with those sourced from studies conducted in other regions of the world. Despite the high morphometric variability, particularly on spiracles in both genders and in female genitalia, data obtained with different genetic molecular markers show very low variability, suggesting the existence of a unique species. In addition, the phylogenetic analysis showed genetic uniformity, supporting the existence of a well-defined clade consisting of R. sanguineus s.l. specimens from Western Europe that are distinct from R. sanguineus s.l. from Africa. Furthermore, these data corroborate the existence of a polymorphic species in Western Europe, which requires to be consensually redescribed in view of its medical and veterinary importance in pathogen transmission.

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Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae)

Cited by 46 publications

References 27 publications

Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal

Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal

Morphological and molecular identification of the brown dog tick Rhipicephalus sanguineus and the camel tick Hyalomma dromedarii (Acari: Ixodidae) vectors of Rickettsioses in Egypt

Mitochondrial DNA analysis of Rhipicephalus sanguineus s.l. from the western Iberian peninsula

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