Molecular Biology of Spermatozoa

Peña, Fernando J.

doi:10.3390/ijms21093060

Cited by 2 publications

(3 citation statements)

References 12 publications

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“…As spermatogenesis progresses under the influence of various molecules, cells, and hormones, spermatozoa are ultimately produced [ 78 ]. Spermatozoa, the key components of the male reproductive system, carry the important task of transmitting genetic information [ 79 ]. Typically composed of a head, neck, and tail, spermatozoa have a highly specialized structure.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of mRNA and lncRNA Expression Profiles in Testicular Tissue of Sexually Immature and Sexually Mature Mongolian Horses

Liu,

Du,

Zhang

et al. 2024

Animals

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Testicular development and spermatogenesis are tightly regulated by both coding and non-coding genes, with mRNA and lncRNA playing crucial roles in post-transcriptional gene expression regulation. However, there are significant differences in regulatory mechanisms before and after sexual maturity. Nevertheless, the mRNAs and lncRNAs in the testes of Mongolian horses have not been systematically identified. In this study, we first identified the testicular tissues of sexually immature and sexually mature Mongolian horses at the tissue and protein levels, and comprehensively analyzed the expression profiles of mRNA and lncRNA in the testes of 1-year-old (12 months, n = 3) and 10-year-old (n = 3) Mongolian horses using RNA sequencing technology. Through gene expression analysis, we identified 16,582 mRNAs and 2128 unknown lncRNAs that are commonly expressed in both sexually immature and sexually mature Mongolian horses. Meanwhile, 9217 mRNAs (p < 0.05) and 2191 unknown lncRNAs (p < 0.05) were identified as differentially expressed between the two stages, which were further validated by real-time fluorescent quantitative PCR and analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). The analysis results showed that genes in the sexually immature stage were mainly enriched in terms related to cellular infrastructure, while genes in the sexually mature stage were enriched in terms associated with hormones, metabolism, and spermatogenesis. In summary, the findings of this study provide valuable resources for a deeper understanding of the molecular mechanisms underlying testicular development and spermatogenesis in Mongolian horses and offer new perspectives for future related research.

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of mRNA and lncRNA Expression Profiles in Testicular Tissue of Sexually Immature and Sexually Mature Mongolian Horses

Liu,

Du,

Zhang

et al. 2024

Animals

View full text Add to dashboard Cite

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“…The spermatozoon, a gamete generated in the male reproductive tract, moves to the female reproductive tract in order to fertilize an egg [65]. In mammals, sperm of the post testicular epididymal tract turns into spermatozoa capable of fertilization [50].…”

Section: Oxidative Stress In Spermatozoamentioning

confidence: 99%

“…As a transcriptionally silent cell, spermatozoa largely depend on post-translational modifications of proteins to regulate their functions. Among these regulatory mechanisms, redox regulation plays a significant role, and the deregulation of redox homeostasis is a cause of male infertility [65].…”

Section: Oxidative Stress In Spermatozoamentioning

confidence: 99%

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants

Pasciu

Nieddu

Sotgiu

et al. 2023

Animals

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The present review aims to provide an overview of the assay methods for the quantification of ROS and principal enzymatic antioxidants as biomarkers of oxidative stress in erythrocytes and spermatozoa of small domestic ruminants. A complete literature search was carried out in PubMed, Scopus and the World Wide Web using relevant keywords and focusing on the last five years (2018–2023). Among spectrophotometry, fluorometry and chemiluminescence, the most widely used method for ROS assay is fluorometry, probably because it allows to simultaneously assay several ROS, using different probes, with greater economic advantages. Regarding intracellular antioxidant enzymes, recent literature reports only spectrophotometric methods, many of which use commercial kits. The use of a less sensitive but cheapest method is suitable because both erythrocytes and spermatozoa samples are highly concentrated in domestic ruminant species. All methods considered in this review have been found to be appropriate; in general, the differences are related to their costs and sensitivity. Quantification of ROS and enzymatic antioxidant activity in erythrocytes and spermatozoa may find application in the study of the welfare and health status of small domestic ruminants for monitoring livestock production.

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Molecular Biology of Spermatozoa

Abstract: The spermatozoon is a very special cell; it is generated in the male reproductive tract and has to travel to the female reproductive tract, of another individual, to fertilize an egg [...]

Cited by 2 publications

References 12 publications

Comparative Analysis of mRNA and lncRNA Expression Profiles in Testicular Tissue of Sexually Immature and Sexually Mature Mongolian Horses

Comparative Analysis of mRNA and lncRNA Expression Profiles in Testicular Tissue of Sexually Immature and Sexually Mature Mongolian Horses

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants

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