Molecular Biomarkers for Embryonic and Adult Neural Stem Cell and Neurogenesis

Zhang, Juan; Jiao, Jianwei

doi:10.1155/2015/727542

Cited by 170 publications

(195 citation statements)

References 152 publications

(204 reference statements)

Supporting

Mentioning

184

Contrasting

Unclassified

Order By: Relevance

“…The expression of Dcx suggested the presence of type 3 cells, which represent a more advanced form of neuronal differentiation. Generally most type 3 cells are Dcx positive and nestin negative [Brandt et al, 2003;Zhang and Jiao, 2015]. Therefore, it should be noted that for cells cultured using the electrospun PLLA MTAM, despite the strong expression of Dcx, a moderate expression of nestin was also observed ( Fig.…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

Tseng¹,

Chew²,

Huang³

et al. 2017

Cells Tissues Organs

View full text Add to dashboard Cite

Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. In this study, the interaction between rat fetal neural stem cells (NSC) and astrocytes was examined by comparing the outcome of a typical Transwell-based coculture system and that of an electrospun PLLA MTAM-based coculture system. Compared to tissue culture polystyrene (TCP) and Transwell coculture inserts, a superior cell viability of NSC was observed when cultured in lumens of electrospun PLLA MTAM (with supportive immunostaining images). Reverse transcription polymerase chain reaction revealed a strong interaction between astrocytes and NSC through a higher expression of doublecortin and a lower expression of nestin. These data demonstrate that MTAM is clearly a better coculture platform than the traditional Transwell system.

show abstract

Section: Discussionmentioning

confidence: 89%

“…Moving on to the expression of Dcx (a form of immature neuronal marker [Zhang and Jiao, 2015], Fig. 6 c), it revealed the highest expression for cells cultured within the electrospun PLLA MTAM, and this was almost 6 times the amount expressed by cells cultured on TCP and using the Transwell insert.…”

Section: Discussionmentioning

confidence: 99%

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

Tseng¹,

Chew²,

Huang³

et al. 2017

Cells Tissues Organs

View full text Add to dashboard Cite

show abstract

“…NSCs can undergo self-renewal, generate identical cells, and are multipotent for the generation of diverse neural lineages, and they participate in the repair and regeneration of the CNS [28, 29]. NSCs have been shown to reside in a hypoxic environment [16].…”

Section: Discussionmentioning

confidence: 99%

Identification and Verification of Candidate Genes Regulating Neural Stem Cells Behavior Under Hypoxia

Shi

Wei

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Neural stem cells (NSCs) reside in a hypoxic environment, and hypoxia plays an important role in their development and differentiation. This study aimed to explore the underlying mechanisms by which hypoxia affects NSC behavior. Methods: In the current study, we downloaded the gene expression dataset GSE68572 and identified the differentially expressed genes (DEGs) by analyzing high-throughput gene expression in hypoxic and normoxic NSCs. Subsequently, we analyzed these data using a combined bioinformatics approach and predicted the microRNAs (miRNAs) targeting the key gene using miRNA databases. Quantitative real-time PCR (qRT-PCR) was used to validate the expression of the top five DEGs. Results: In total, 1347 genes were identified as DEGs. We identified the predominant gene ontology categories and Kyoto Encyclopedia of Genes and Genomes pathways that were significantly over-represented in the hypoxic NSCs. A protein–protein interaction network he identification of miRNAs and their putative targets may offer new diagnostic and therapeutic strategies for liver cancer the top 10 core genes. Vascular endothelial growth factor A (VEGFA) had the highest degree and may be the key gene concerning NSC behavior under hypoxia. Further validation of the top five DEGs by qRT-PCR demonstrated that four DEGs were significantly higher and one DEG was significantly lower in the hypoxic group than in the control group. Seven miRNAs were predicted and proved to target VEGFA. Conclusion: This preliminary study can prompt the understanding of the molecular mechanisms by which hypoxia has an impact on NSC behavior and can help to optimize stem cell therapies for central nervous system injuries and diseases.

show abstract

“…miR-3666 can regulate proper cortical patterning through regulation of the common target gene EMX2 (empty spiracles homolog 2) and miR-3666 target PAX6 (paired box 6). The initial regional pattern of the neocortex is established by the distinct spatial distribution of PAX6 and EMX2 [69]. Additionally, model 2 ( Figure 5b) demonstrates miR-3666 may enhance FOXP2 suppression of LMO4 (LIM domain-only 4) by inhibiting the common target gene, CREB1 (cAMP responsive element binding protein 1).…”

Section: Mir-3666 Directly or Indirectly Regulates Foxp2 Functions Inmentioning

confidence: 99%

“…We also studied FOXP2 interactions using STRING [36,37] (Figure 3a), BioGRID [38] and IntAct [64]. For a more extensive study, we referred scientific literature [60][61][62][63][64][65][66][67][68][69] and took into consideration some genes that were not common targets but play important roles in the nervous system or in pathogenesis of neurological disorders (for example, CNTNAP2 and BDNF) and showed potential in being indirectly co-regulated by FOXP2 and miR-3666 through common target genes. Therefore, a total of 30 genes were selected for developing our models (Supplementary Table S4).…”

Section: Four Models Developed Show Mechanisms By Which Mir-3666 Mighmentioning

confidence: 99%

Intronic miRNA miR-3666 Modulates its Host Gene FOXP2 Functions in Neurodevelopment and May Contribute to Pathogenesis of Neurological Disorders Schizophrenia and Autism

Islam¹

2017

JABB

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are approximately 22-nucleotide-long, non-coding RNAs that bind to complementary mRNAs with inhibitory effect. An intronic miRNA is embedded in a particular gene called its host gene. Our study focuses on the Homo sapiens intronic miRNA-host gene pair, hsa-miR-3666 and FOXP2. Previous report of coexpression of miR-3666 and FOXP2 indicates possible regulation of FOXP2 functions by miR-3666. However, direct correlation has not been shown yet. Therefore, we took a computational approach to determine if and how such modulation occurs. ChIP-seq identified FOXP2 targets and putative miR-3666 targets showed a significant overlap of 574 common target genes. Functional enrichment analysis of common targets revealed over-representation of KEGG pathways and Gene Ontology modules associated with neurodevelopment. These modules, along with further literature mining and proteinprotein interaction analysis of FOXP2 and miR-3666 identified several specific genes associated with neurodevelopment and finally integration of transcriptomic expressions data lead to the selection of four models depicting the mechanisms by which miR-3666 can modulate FOXP2 functions. Model 1 illustrates that during neurodevelopment, miR-3666 can directly modulate the functions of FOXP2 through regulation of common targets, such as IGF1 and EFNB2, whereas model 2 shows miR-3666 can also indirectly modulate FOXP2 functions by considering targets that are not common for the intronic miRNA-host gene pair, for example CDH2 and LMO4. This direct and indirect regulation is necessary for precise spatial and temporal expression of genes during neurodevelopment. Models 3 and 4 exhibit mechanisms in which the interactions of miR-3666 and FOXP2 with target genes contribute to the pathogenesis of schizophrenia and autism respectively. a role in transcriptional activation [7]. FOXP2 hosts the intronic miRNA, miR-3666. Though not much work has been done on miR-3666, its targets have been predicted and deposited in various databases. A few recent experiments have shown the repression activity of miR-3666 on targets such as MET and ZEB1 in thyroid carcinoma and cervical carcinoma cells, respectively [9,10].

show abstract

Molecular Biomarkers for Embryonic and Adult Neural Stem Cell and Neurogenesis

Cited by 170 publications

References 152 publications

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

Identification and Verification of Candidate Genes Regulating Neural Stem Cells Behavior Under Hypoxia

Intronic miRNA miR-3666 Modulates its Host Gene FOXP2 Functions in Neurodevelopment and May Contribute to Pathogenesis of Neurological Disorders Schizophrenia and Autism

Contact Info

Product

Resources

About