Molecular biomonitoring of a population of nurses handling antineoplastic drugs

Cornetta, Tommaso; Padua, Luca; Testa, Antonella; Ievoli, Elena; Festa, Fabiola; Tranfo, Giovanna; Baccelliere, Luigi; Cozzi, Renata

doi:10.1016/j.mrfmmm.2007.08.017

Cited by 54 publications

(36 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taking the overall results of our two monitoring studies into consideration, we have observed that: 1) environmental (surface/clothing) contamination levels were found to be similar to those reported for other Italian hospitals 38) , even with a lower number of positive samples; 2) a very low number of urine samples showed detectable concentrations of the marker compound (i.e., cyclophosphamide), a result in agreement with recent findings indicating surface contamination as not necessarily correlating with positive urine samples 38) , probably because of suboptimal sensitivity of the adopted assays to detect lower concentrations of the drugs in urine; 3) an increased extent of primary DNA damage was observed in exposed subjects handling ANPDs, a positive finding in line with the results from several other studies 14,17,19,39,40) and 4) no significant differences were found for MN frequencies when comparing nurses handling ANPDs and controls (CBMN protocol), in line with previously published studies 11−15) , but an adaptive response in human lymphocytes from subjects with occupational exposure to ANPDs (CBMN/Ara-C protocol) was observed.…”

Section: Discussionsupporting

confidence: 91%

Biological Effect Monitoring in Peripheral Blood Lymphocytes from Subjects Occupationally Exposed to Antineoplastic Drugs: Assessment of Micronuclei Frequency

Villarini

Dominici

Fatigoni

et al. 2012

Journal of Occupational Health

View full text Add to dashboard Cite

Biological Effect Monitoring in Peripheral Blood Lymphocytes from Subjects Occupationally Exposed to Antineoplastic Drugs: Assessment of Micronuclei Frequency: Milena VILLARINI, et al. Department of Medical‐Surgical Specialties and Public Health (Section of Public Health), University of Perugia, Italy— Objectives Antineoplastic drugs (ANPDs) are widely used in the treatment of cancer and some nonneoplastic diseases. However, most if not all of these chemical agents are generally nonselective and, along with tumor cells, normal cells may undergo cytotoxic/genotoxic damage. Italian pharmacists and nurses occupationally exposed to ANPDs during their normal work routines were monitored to evaluate biological effects (i.e., cytogenetic damage) eventually associated with exposure. The subjects were also monitored for primary, oxidative and excision repaired DNA damage as evaluated by comet assay (published data). In the present paper, we present the results obtained with the cytokinesis‐block micronucleus (CBMN) test. Methods The CBMN test in peripheral blood lymphocytes was applied because of its ability to detect both clastogenic and aneugenic effects, and because it has recently been reported that micronuclei (MNs) are predictive of cancer risk in human populations. In this study, the evaluation of MN frequency was carried out using the CBMN test in the absence or in the presence of the DNA repair inhibitor Ara‐C (cytosine arabinoside). Results No significant difference was observed for MN frequency comparing nurses handling ANPDs (exposed subjects) and controls; no correlations were found between job seniority, age, smoking habits and MN rates. Conclusions Concerning the aim of this study to evaluate the genotoxic risk arising from occupational exposure to ANPDs, statistically significant differences in MN rates in the subjects under study could not be determined.

show abstract

Section: Discussionsupporting

confidence: 91%

Biological Effect Monitoring in Peripheral Blood Lymphocytes from Subjects Occupationally Exposed to Antineoplastic Drugs: Assessment of Micronuclei Frequency

Villarini

Dominici

Fatigoni

et al. 2012

Journal of Occupational Health

View full text Add to dashboard Cite

show abstract

“…However, several studies on populations Environmental and Molecular Mutagenesis. DOI 10.1002/em occupationally exposed to antineoplastics did not show significant differences between smoking and nonsmoking subjects in terms of MN frequency or DNA damage by comet assay [Undeger et al, 1999;Kopjar and GarajVrhovac, 2001;Cornetta et al, 2008], and our findings on workers exposed to antineoplastic drugs are in agreement with such studies. Some studies on populations occupationally exposed to PAHs did not find any effect of smoking on the MN frequency or DNA damage by comet test [Bolognesi et al, 1997;Pitarque et al, 1999], and this was consistent with our results on paving workers.…”

Section: Discussionsupporting

confidence: 92%

Evaluation of a suitable DNA damage biomarker for human biomonitoring of exposed workers

Cavallo¹,

Ursini²,

Rondinone³

et al. 2009

Environ and Mol Mutagen

View full text Add to dashboard Cite

The objective of this study was to identify a sensitive and noninvasive biomarker of early genotoxic effects, for health risk assessment of workers exposed to mixtures of low doses of xenobiotics. We studied 30 workers exposed to antineoplastic drugs, 57 workers exposed to different mixtures of polycyclic aromatic hydrocarbons (PAHs) (41 airport workers and 16 paving workers) and 76 controls. Comet and micronucleus (MN) tests were performed on lymphocytes and exfoliated buccal cells. The MN assay on lymphocytes did not show significant differences between exposed and controls, while the MN assay on exfoliated buccal cells showed higher values in workers exposed to antineoplastics as compared with controls (0.85 vs. 0.48, P = 0.042). The comet assay on lymphocytes showed a higher comet percentage value (18.11 vs. 11.24 in controls, P = 0.001) and mean tail moment (TM) value (21.84 vs. 16.72 in controls, P = 0.003) in individuals exposed to PAHs as compared with controls; no significant differences were found in exposed to antineoplastics. The comet assay on exfoliated buccal cells did not show significant differences between exposed and control groups for comet percentages, whereas the TM value was higher in workers exposed to PAHs (55.1 vs. 32.31 for controls, P < 0.001). These results show that exfoliated buccal cells, obtained by a noninvasive procedure, represent robust target cells to assess the occupational exposure to inhalable mixture of chemicals at low doses. The comet assay seems to be suitable to promptly evaluate the genotoxic effects of PAHs mixtures that also contain volatile substances. The MN test is suitable to evaluate the effects of antineoplastics. Inc.

show abstract

“…Consistently, some studies reported increased frequencies of micronuclei in peripheral blood lymphocyes [Rekhadevi et al, 2007;Cornetta et al, 2008] and/or exfoliated buccal cells [Cavallo et al, 2007;Rekhadevi et al, 2007] with respect to matched controls. To our knowledge, only 1 study employed an aneuploidy-specific biomarker: FISH analysis with centromeric probes was applied to a small sample of observed micronuclei to characterise their mechanism of origin [Cavallo et al, 2007], and the authors claimed an association between vinorelbine exposure and centromeric-positive micronuclei, but the sample is too small to draw any firm conclusion.…”

Section: Cytostatic Drugs In Healthcare Occupational Settingssupporting

confidence: 56%

Environmental Hazard in the Aetiology of Somatic and Germ Cell Aneuploidy

Pacchierotti

Eichenlaub-Ritter

2011

Cytogenet Genome Res

View full text Add to dashboard Cite

Sources of environmental exposures to potentially aneugenic agents are many and include occupational and therapeutic exposures, and exposures associated with lifestyle habits. In this present study, some of these agents and exposure scenarios are discussed that involve potentially large population targets and/or seem to affect chromosome segregation by previously unsuspected mechanisms: metals, possibly acting by epigenetic mechanisms; nano-sized particles that might directly interact with subcellular components of the mitotic and meiotic machineries; cytostatic drugs in healthcare occupations; anticancer therapies potentially affecting the genetic integrity of gametes; continuously increasing electromagnetic field exposures with some sparse evidence of aneugenic activity; endocrine disruptors and their seemingly elusive effects in mouse oocytes, including the first evidence that prenatal exposure could affect meiotic nondisjunction in adult life. Hazards are considered for both somatic cells at risk of neoplastic transformation or tumour progression by chromosome loss and gain and germ cells at risk of heritable aneuploidies associated with spontaneous abortions or genetic diseases. Finally, possible synergistic interactions between environmental exposure and ageing or genetic predisposition are considered that could influence ultimate risks.

show abstract

Molecular biomonitoring of a population of nurses handling antineoplastic drugs

Cited by 54 publications

References 39 publications

Biological Effect Monitoring in Peripheral Blood Lymphocytes from Subjects Occupationally Exposed to Antineoplastic Drugs: Assessment of Micronuclei Frequency

Biological Effect Monitoring in Peripheral Blood Lymphocytes from Subjects Occupationally Exposed to Antineoplastic Drugs: Assessment of Micronuclei Frequency

Evaluation of a suitable DNA damage biomarker for human biomonitoring of exposed workers

Environmental Hazard in the Aetiology of Somatic and Germ Cell Aneuploidy

Contact Info

Product

Resources

About