Molecular Breeding in Coffee (Coffea Arabica L.)

Lebailly, Philippe; Combes, Marie‐Christine; Topart, Patrick; Graziosi, G.; Bertrand, Benoît; Anthony, François

doi:10.1007/978-94-017-1068-8_7

Cited by 18 publications

(20 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, Orozco-Castillo et al (1994) successfully used RAPD markers to discriminate Coffea genotypes belonging to different subgroups. AFLP also exposed low genetic variability in arabica accessions, while a considerable level of polymorphic markers was revealed among Timor Hybrid-derived genotypes, which were associated to introgression of genes from the C. canephora gene pool (Lashermes et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

Genetic polymorphism among 14 elite Coffea arabica L. cultivars using RAPD markers associated with restriction digestion

Sera

Ruas

et al. 2003

Genet. Mol. Biol.

View full text Add to dashboard Cite

Knowledge of the genetic variability among genotypes is important for the transfer of useful genes and to maximize the use of available germplasm resources. This study was carried out to assess the genetic variability of 14 elite Coffea arabica cultivars using random amplified polymorphic DNA (RAPD) associated with a prior digestion of genomic DNA with restriction endonucleases. The accessions were obtained from the Coffea collection maintained at the Instituto Agronômico do Paraná (IAPAR), located in Londrina, Paraná, Brazil. Twenty-four informative RAPD primers, used in association with restriction enzymes, yielded 330 reproducible and scorable DNA bands, of which 224 (68%) were polymorphic. The amplified products were used to estimate the genetic variability using Dice's similarity coefficient. The data matrix was converted to a dendrogram and a three-dimensional plot using principal coordinate analysis. The accessions studied were separated into clusters in a manner that was consistent with the known pedigree. The associations obtained in the dendrogram and in the principal coordinate analysis plot suggest the probable origin of the Kattimor cultivar. The RAPD technique associated with restriction digestion was proved to be a useful tool for genetic characterization of C. arabica genotypes making an important contribution to the application of molecular markers to coffee breeding.

show abstract

Section: Resultsmentioning

confidence: 99%

Genetic polymorphism among 14 elite Coffea arabica L. cultivars using RAPD markers associated with restriction digestion

Sera

Ruas

et al. 2003

Genet. Mol. Biol.

View full text Add to dashboard Cite

show abstract

“…The chromosomes of coffee species are rather uniform in shape and size (Lashermes et al 1999). If we assume a mean length of 100 cM per chromosome in C. arabica (Lashermes et al 2000a) and the lack of significant recombination restriction in the region of introgressed fragments, as reported for C. canephora chromosome segments (Herrera et al 2002), one can estimate that the introgression of C. liberica chromosome fragments in S.288 represents approximately one-half the chromosome equivalent of C. liberica distributed over two or three chromosomes.…”

Section: Discussionmentioning

confidence: 98%

“…However, the combined introgression into outstanding cultivars of various genes of resistance appears to be a very difficult task in an acceptable time-frame through traditional breeding approaches. An availability of molecular markers linked to S H genes and other genes conditioning resistance to coffee leaf rust would be extremely useful, especially in the context of the gene pyramiding approach (Santaram and Sreenath 2000;Lashermes et al 2000a;Van der Vossen 2001).…”

Section: Introductionmentioning

confidence: 99%

Introgression molecular analysis of a leaf rust resistance gene from Coffea liberica into C. arabica L.

Prakash¹,

Marques

Várzea

et al. 2004

Theor Appl Genet

Self Cite

View full text Add to dashboard Cite

Leaf rust caused by the fungus Hemileia vastatrix is the most devastating disease of arabica coffee ( Coffea arabica). Therefore, developing leaf rust-resistant varieties has been a breeding objective of the highest priority in many countries. The purpose of the present work was to gain insight into the mechanism of introgression into C. arabica of a leaf rust resistance gene from C. liberica (i.e. S(H)3 resistance factor) and to identify associated molecular markers. An F(2) progeny (i.e. 101 individuals) derived from a cross between Matari, an arabica accession and liberica-introgressed line S.288, was evaluated for resistance against three different races of H. vastatrix. The progeny segregated for the S(H)3 gene in a 3:1 ratio, as expected for a single dominant gene. Amplified fragment length polymorphism analysis of a population subset using 80 different primer combinations revealed that at least half of the total polymorphism observed in the population is associated with introgression of C. liberica chromosome fragments. Furthermore, 15 primer combinations generating candidate marker bands associated with the S(H)3 resistance gene were used to analyse the whole F(2) population. A total of 34 marker bands originating from S.288 and attributable to introgression were scored. None exhibited segregation distortion. Linkage analysis revealed only three distinct introgressed fragments corresponding to a total length of 52.8 cM. Twenty-one markers were strongly associated (LOD score >14) with the S(H)3 gene and were grouped together in a single linkage group of 6.3 cM. The results are discussed in relation to the efficient use of genetic resources in arabica breeding.

show abstract

“…This is an encouraging result for the future of genetic improvement programmes based on the introgression of resistance genes from C. canephora via the Timor Hybrid. However, if it is to be more effective and, in particular, if it is to avoid maintaining undesirable introgressed fragments suspected of having a negative effect on BQ, selection could be assisted by specific markers of resistance to pests/diseases (Lashermes et al 2000b). This programme would be much more efficient if it possible to detect chemical compounds with variations that are highly correlated to quality defects attributable to introgression.…”

Section: Discussionmentioning

confidence: 98%

Impact of the Coffea canephora gene introgression on beverage quality of C. arabica

Bertrand¹,

et al. 2003

Self Cite

View full text Add to dashboard Cite

Lines of Coffea arabica derived from the Timor Hybrid (hybrid between C. arabica and C. canephora) are resistant to coffee leaf rust (Hemileia vastatrix) and to the nematode Meloidogyne exigua. The introgression of C. canephora resistance genes is suspected of causing a drop in beverage quality. Coffee samples from pure lines, compared in a Trial 1, and from F1 hybrids and parental lines from a half-diallel trial in a Trial 2, were studied for beverage quality, chemical composition and amount of introgressed genetic material. Chemical analyses (caffeine, chlorogenic acids, fat, trigonelline, sucrose) were carried out with near-infrared spectrometry by reflectance of green coffee. The number of amplified fragment length polymorphic (AFLP) markers introgressed from the Timor Hybrid varied from 1 to 37 for the lines studied. There were significant differences between lines for all of the biochemical compounds analysed and for the acidity and the overall standard of the beverage. Two lines (T17927, T17924) were significantly poorer than the controls for sucrose and beverage acidity. T17924 also had more chlorogenic acids and was poorer for the overall standard. However, two highly introgressed lines, T17934 and T17931 (25 and 30 AFLP markers, respectively), did not differ from the non-introgressed controls. There were no correlations between the number of AFLP markers and the chemical contents or beverage attributes. Significant correlations were found between the performance of the parents and their general combining ability for beverage quality. It was concluded that it should be possible to find lines with both the desired resistance genes and good beverage quality. Selection can avoid accompanying the introgression of resistance genes with a drop in beverage quality.

show abstract

Molecular Breeding in Coffee (Coffea Arabica L.)

Cited by 18 publications

References 18 publications

Genetic polymorphism among 14 elite Coffea arabica L. cultivars using RAPD markers associated with restriction digestion

Genetic polymorphism among 14 elite Coffea arabica L. cultivars using RAPD markers associated with restriction digestion

Introgression molecular analysis of a leaf rust resistance gene from Coffea liberica into C. arabica L.

Impact of the Coffea canephora gene introgression on beverage quality of C. arabica

Contact Info

Product

Resources

About