Molecular breeding of “Swarna,” a mega rice variety for lodging resistance

Merugumala, Girija Rani; P.V, Satyanarayana; Chamundeswari, N.; B.N.V.S.R, Ravikumar; Rao, R. Sudhakara; Pavani, Luiz Carlos; Deepika, V

doi:10.1007/s11032-019-0961-z

Cited by 12 publications

(6 citation statements)

References 34 publications

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“…The popular mega rice variety, Swarna has been widely adopted by farmers for its high yielding ability and adaption under low input conditions, shallow low lands. It is cultivated in an approximate area of 4.5 M ha 21 , occupying more than 30% of total rice area in eastern India 22 . Swarna possesses good grain and cooking quality with desirable grain type.…”

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confidence: 99%

Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Ponnuswamy

Singh

Raman

et al. 2020

Sci Rep

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the major constraints in hybrid rice breeding are availability of limited number of parental lines with specific desirable traits and lower frequency of restorers among elite breeding lines. The popular, high-yielding mega-rice variety Swarna, has been identified to be a partial restorer (as it has only one of major fertility restorer genes, Rf4) and hence cannot be utilized directly in the hybrid rice breeding. To convert the partial restorer to complete restorer, a cross was made between Swarna and a stable restorer KMR3R possessing Rf3 and Rf4 genes and developed BC 1 f 5 and Bc 2 f 4 populations by markerassisted back cross breeding (MABB). The SSR marker DRRM-RF3-10 linked to Rf3 gene located on chromosome 1, clearly distinguished restorers from partial restorers. All the improved lines of Swarna possessing Rf3 and Rf4 genes showed complete fertility restoration in test crosses with higher grain yield heterosis. few rice hybrids developed by using converted restorers were evaluated in multi location testing under the All india co-ordinated Rice improvement project (AicRip). the results indicated that new rice hybrids expressed higher heterosis with matching grain quality attributes like Swarna. This study provides significantly novel and relevant restorers to enhance and economize future hybrid rice breeding programs.Rice (O. sativa L.) is the most important staple food crop for more than half of the world's population and it is cultivated in an area of 44.5 million hectare in India with the production of 106.5 million tonnes during the year 2016 1 . After the advent of high yielding semi-dwarf rice varieties, hybrid rice technology has been touted as a major strategy for enhancing the genetic yield potential of rice. The success of hybrid rice technology has been very well demonstrated in China, which produces 146.5 million tonnes of rice from 30.32 million hectares 1 . This significant increase in production in China is mainly due to cultivation of hybrid rice (with >50% area and production under rice hybrids). Several technical challenges, market and policy constraints has limited the development and diffusion of hybrid rice outside China 2 . In India, hybrid rice is cultivated in an area of ~3 million hectares 3 , which is about 6.7% of total area of rice cultivation. Hybrid rice accounts for less than 10% of the area under rice cultivation in Bangladesh, Indonesia, and the Philippines and just 10% in Vietnam.Hybrid rice technology aims to increase the yield potential of rice by exploiting the phenomenon of hybrid vigour or heterosis. Cytoplasmic male sterility coupled with fertility restoration controlled by nuclear genes is a very useful tool in exploiting heterosis in self pollinated crops. In rice, three CMS systems viz. Wild Abortive (WA), Boro II (BT) and Honglian (HL) are deployed for commercial hybrid rice seed production 4,5 . The most widely used CMS system in rice is based on wild abortive (WA) cytoplasm derived from Oryza sativa f. Spontanea 6,7 . The WA-CMS system is highly stable with compl...

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Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Ponnuswamy

Singh

Raman

et al. 2020

Sci Rep

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“…All the standard agronomic practices were followed and data were documented for three plants selected randomly from each accession for the target trait stem girth (mm). Digital Vernier callipers were used to measure the stem girth at the fourth inter-node at 20 days after heading (Merugumala et al, 2019). Descriptive statistics and frequency distribution of the whole population for stem girth was performed using Minitab statistical software (Minitab, Inc. 2010) Allelic diversity in the entire population was performed by downloading their genomic sequences from Rice SNP Seek Database (Mansueto et al, 2017) and the multi sequence alignment was done using Bioedit software (Hall et al, 1999) using Nipponbare as a reference genome.…”

Section: Methodsmentioning

confidence: 99%

Haplotype analysis of SCM2 loci for identification of donor for lodging resistance in rice

Madhuri¹,

Manonmani²,

Thiyagarajan³

et al. 2021

EJPB

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Lodging resistance is one of the vital traits in improving the yield and sustainability of production in rice (Oryza sativa L.). A quantitative trait loci (QTL), SCM2 for enhancing the culm strength and increased spikelet number has been reported. In the present study, a diverse collection of 150 accessions from the rice 3K-RG panel were assembled and evaluated for the STRONG CULM2 locus. All the accessions under study showed a normal phenotypic distribution for culm diameter and the allelic diversity analysis for SCM2 resulted in four INDEL's and 17 SNP's in both coding and non-coding regions of the genome. Haplotype analysis grouped the entire population into three significant haplotype groups with 114 accessions in H1, 19 accessions in H2 and 17 accessions in H3. Haplotypes were formed using two significant SNP sites viz., 27480778 and 27481339. Dunnett's test disclosed a significant variation between H1-H2 and H1-H3 haplotypes. Phenotypic evaluation for culm diameter in all the three haplotype groups revealed that the genotypes BR IRGA and PERUNEL in H1, CICA 9 and MOSHI in H2 and KITRANA in H3 possess a maximum culm diameter. H2 and H3 were found to be superior to H1 hence can be utilized for lodging resistance in further breeding programs.

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“…(2019) was found to possess putative genes responsible for cytokinin signalling, cell cycle signalling, NAC transcription factor signalling and other biological processes. Two QTLs, qCd6 (culm diameter) and qCt6 (culm thickness), incongruent with SCM2/APO1 and qBs2 (bending stress) are reported in the vicinity of SCM4 (Merugumala et al ., 2019). The GWAS on a complex mapping population for DDSR traits identified QTLs for culm diameter and stem diameter (chromosome 3) co‐located with bc3 and SCM3 , respectively (Sandhu et al ., 2019a).…”

Section: Traits For Developing Ddsr Varietiesmentioning

confidence: 99%

More and more of less and less: Is genomics‐based breeding of dry direct‐seeded rice (DDSR) varieties the need of hour?

Sagare¹,

Abbai

Jain³

et al. 2020

Plant Biotechnology Journal

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Rice is a staple food for half of the world's population. Changing climatic conditions, water and labour scarcity are the major challenges that shall limit future rice production. Dry direct-seeded rice (DDSR) is emerging as an efficient, resources conserving, mechanized, climate smart and economically viable strategy to be adopted as an alternative to puddled transplanted rice (TPR) with the potential to address the problem of labour-water shortages and ensure sustainable rice cultivation. Despite these benefits, several constraints obstruct the adoption of DDSR. In principle, the plant type for DDSR should be different from one for TPR, which could be achieved by developing rice varieties that combine the traits of upland and lowland varieties. In this context, recent advances in precise phenotyping and NGS-based trait mapping led to identification of promising donors and QTLs/genes for DDSR favourable traits to be employed in genomic breeding. This review discusses the important traits influencing DDSR, research studies to clarify the need for breeding DDSR-specific varieties to achieve enhanced grain yield, climate resilience and nutrition demand. We anticipate that in the coming years, genomic breeding for developing DDSR-specific varieties would be a regular practice and might be further strengthened by combining superior haplotypes regulating important DDSR traits by haplotypebased breeding.

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Molecular breeding of “Swarna,” a mega rice variety for lodging resistance

Cited by 12 publications

References 34 publications

Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Haplotype analysis of SCM2 loci for identification of donor for lodging resistance in rice

More and more of less and less: Is genomics‐based breeding of dry direct‐seeded rice (DDSR) varieties the need of hour?

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