Molecular chaperones in protein folding and stress management in cyanobacteria

Chatterjee, Aroni; Rajarshi, Keshav; Ghosh, Hiya; Singh, Mrityunjay; Roy, O P; Ray, Shashwati

doi:10.1016/b978-0-12-819311-2.00008-5

Cited by 7 publications

(7 citation statements)

References 54 publications

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“…While this gene is solitary placed on the genome another copy of the gene, groL1 (_61181), is accompanied by its cochaperonin-encoding groS gene (_61182). Chaperonins promote protein folding and are known to play a role in the maintenance of cellular stability under a wide variety of stress [ 50 ]. Though most cyanobacteria encode one groSL locus and one additional monocistronic groL many also contain a second groSL [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Genetic Responses of Metabolically Active Limnospira indica Strain PCC 8005 Exposed to γ-Radiation during Its Lifecycle

et al. 2021

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Two morphotypes of the cyanobacterial Limnospira indica (formerly Arthrospira sp.) strain PCC 8005, denoted as P2 (straight trichomes) and P6 (helical trichomes), were subjected to chronic gamma radiation from spent nuclear fuel (SNF) rods at a dose rate of ca. 80 Gy.h−1 for one mass doubling period (approximately 3 days) under continuous light with photoautotrophic metabolism fully active. Samples were taken for post-irradiation growth recovery and RNA-Seq transcriptional analysis at time intervals of 15, 40, and 71.5 h corresponding to cumulative doses of ca. 1450, 3200, and 5700 Gy, respectively. Both morphotypes, which were previously reported by us to display different antioxidant capacities and differ at the genomic level in 168 SNPs, 48 indels and 4 large insertions, recovered equally well from 1450 and 3200 Gy. However, while the P2 straight type recovered from 5700 Gy by regaining normal growth within 6 days, the P6 helical type took about 13 days to recover from this dose, indicating differences in their radiation tolerance and response. To investigate these differences, P2 and P6 cells exposed to the intermediate dose of gamma radiation (3200 Gy) were analyzed for differential gene expression by RNA-Seq analysis. Prior to batch normalization, a total of 1553 genes (887 and 666 of P2 and P6, respectively, with 352 genes in common) were selected based on a two-fold change in expression and a false discovery rate FDR smaller or equal to 0.05. About 85% of these 1553 genes encoded products of yet unknown function. Of the 229 remaining genes, 171 had a defined function while 58 genes were transcribed into non-coding RNA including 21 tRNAs (all downregulated). Batch normalization resulted in 660 differentially expressed genes with 98 having a function and 32 encoding RNA. From PCC 8005-P2 and PCC 8005-P6 expression patterns, it emerges that although the cellular routes used by the two substrains to cope with ionizing radiation do overlap to a large extent, both strains displayed a distinct preference of priorities.

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Section: Resultsmentioning

confidence: 99%

Genetic Responses of Metabolically Active Limnospira indica Strain PCC 8005 Exposed to γ-Radiation during Its Lifecycle

et al. 2021

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“…GSH depletion in eukaryotes can be explained by its extensive consumption in the ER lumen to repair disulfides formed as a consequence of high temperatures ( Distéfano et al, 2017 ; Ozgur et al, 2014 ). Although cyanobacteria lack the ER, it was proposed that the process could be very similar in these organisms ( Chatterjee et al, 2020 ; Narainsamy et al, 2016 ). Additionally, when GSH pool is depleted, AsA cannot be recycled via the thiol-dependent mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Several key genes, metabolites, and processes relevant to ferroptosis described in humans (Dixon et al, 2012;Conrad et al, 2018) are shown as well as a summary of their potential role in cyanobacteria. that the process could be very similar in these organisms (Chatterjee et al, 2020;Narainsamy et al, 2016). Additionally, when GSH pool is depleted, AsA cannot be recycled via the thioldependent mechanisms.…”

Section: Autophagymentioning

confidence: 97%

C-ferroptosis is an iron-dependent form of regulated cell death in cyanobacteria

Aguilera

Berdun

Bártoli

et al. 2021

Journal of Cell Biology

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Ferroptosis is an oxidative and iron-dependent form of regulated cell death (RCD) recently described in eukaryotic organisms like animals, plants, and parasites. Here, we report that a similar process takes place in the photosynthetic prokaryote Synechocystis sp. PCC 6803 in response to heat stress. After a heat shock, Synechocystis sp. PCC 6803 cells undergo a cell death pathway that can be suppressed by the canonical ferroptosis inhibitors, CPX, vitamin E, Fer-1, liproxstatin-1, glutathione (GSH), or ascorbic acid (AsA). Moreover, as described for eukaryotic ferroptosis, this pathway is characterized by an early depletion of the antioxidants GSH and AsA, and by lipid peroxidation. These results indicate that all of the hallmarks described for eukaryotic ferroptosis are conserved in photosynthetic prokaryotes and suggest that ferroptosis might be an ancient cell death program.

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“…Genes known to be involved in resistance mechanisms against stress (Rajeev et al 2013; Soule et al 2013; Shimura et al 2015; Al-Hosani et al 2015; Kopf et al 2015; Ferreira et al 2016; Sinetova et al 2016; Chrismas et al 2016, 2018; Klicki et al 2018; Shang et al 2019; Pathak et al 2019; Kvíderová et al 2019; Pereira et al 2009, 2019; Chatterjee et al 2020; Mosca et al 2021; Ye et al 2021; Napoli et al 2021) were searched in the generated contig database and classified in the following groups: (i) Production of the EPS matrix, (ii) Photoprotection, (iii) Oxidative stress protection, (iv) DNA repair and (v) Osmotic stress protection. When genes were not found in the database, they were searched manually using tblastn against the ULC029 assembled genome with an e-value threshold of 1e-5 and a score higher than 50 bits (following Pearson et al 2013).…”

Section: Methodsmentioning

confidence: 99%

“…If these protective strategies do not succeed, the strategies to revert cellular damages are activated. Cyanobacteria can repair DNA damage by several mechanisms, such as the homologous recombination (HR) or the nucleotide excision repair (NER) pathways (Pathak et al 2019), or refold proteins thanks to chaperones, such as GroEL/GroES or DnaK/J (Chatterjee et al 2020). To revert ROS formation, they also produce oxygen-scavenging proteins, such as catalases or superoxide dismutases (Kvíderová et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Ecophysiological and genomic approaches to cyanobacterial hardening for soil restoration

Beatriz,

Valentina,

Benoit

et al. 2023

Preprint

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Cyanobacteria inhabit extreme environments, including drylands, providing multiple benefits to the ecosystem. Soil degradation in warm drylands is increasing due to land-use intensification. Restoration methods adapted to the strong stress in drylands are being developed, i.e. cyanobacterial inoculation to recover biocrusts. For success, it is crucial to optimize the survival of inoculated cyanobacterial in field. One strategy is to harden them to be re-adapted to stressful conditions after laboratory culturing. Here, we analyzed the genome and ecophysiological response to osmotic, desiccation and UVR stresses of an Antarctic cyanobacterium, Stenomitos frigidus ULC029, closely related to other cyanobacteria from warm and cold dryland soils. Chlorophyll a concentrations show that preculturing ULC029 under moderate osmotic stress improved its survival during an assay of desiccation plus rehydration under UVR. Besides, its sequential exposition to these stress factors increased the production of exopolysaccharides, carotenoids and scytonemin. Desiccation, but not osmotic stress, increased the concentrations of the osmoprotectants, trehalose and sucrose. However, osmotic stress might induce the production of other osmoprotectants, for which the complete pathways were found in the ULC029 genome. In total, 140 genes known to be involved in stress resistance were annotated and could potentially help ULC029 under stress. Here, we confirm that the sequential application of moderate osmotic stress and dehydration, could improve cyanobacterial hardening for soil restoration, by inducing several resistance mechanisms. We provide a high-quality genome of ULC029 and a description of the main resistance mechanisms found (i.e. production of exopolysaccharides, osmoprotectants, chlorophyll and carotenoids; DNA repair; oxidative stress protection).

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Molecular chaperones in protein folding and stress management in cyanobacteria

Cited by 7 publications

References 54 publications

Genetic Responses of Metabolically Active Limnospira indica Strain PCC 8005 Exposed to γ-Radiation during Its Lifecycle

Genetic Responses of Metabolically Active Limnospira indica Strain PCC 8005 Exposed to γ-Radiation during Its Lifecycle

C-ferroptosis is an iron-dependent form of regulated cell death in cyanobacteria

Ecophysiological and genomic approaches to cyanobacterial hardening for soil restoration

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