Molecular characterisation and similarity relationships among iranian basil (Ocimum basilicum L.) accessions using inter simple sequence repeat markers

Aghaei, Mohammad Jaffar; Darvishzadeh, Reza; Hassani, Abbas

doi:10.1590/s1806-66902012000200014

Cited by 19 publications

(12 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many studies have proved the effectiveness of this marker in articles on genetic diversity and characterization of accessions between and within populations, such as those with Ocimum basilicum (Aghaei et al, 2012), Pitcairnia flammea (Souza-Sobreira et al, 2015), Croton heliotropiifolius (Rocha et al, 2016), Erythrina velutina (Souza et al, 2016), and Varronia curassavica (Brito et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira¹,

Silva²,

Alves³

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Brazil has about 300 Croton species in different types of vegetation. Croton tetradenius Baill., which is endemic to the Northeast region and predominant in the Caatinga vegetation, stands out among the several species of this genus. Considering the importance of knowing the genetic variability of a species, the objective of this study was to analyze the genetic diversity of the genotypes of natural populations of C. tetradenius in the State of Sergipe, using ISSR molecular markers. Forty individuals were sampled in four natural populations of the State of Sergipe, Brazil. Thirteen primers were used for DNA amplification using ISSR-PCR, totaling 77 amplified fragments, of which 94.8% were polymorphic. Results of the cluster analysis obtained by the Jaccard's similarity index, using the UPGMA method, resulted in the formation of six distinct clusters. Analysis of molecular variance (AMOVA), used to estimate the genetic variability among populations, revealed significant genetic variance (P < 0.01) between and within the 2 C.S. Almeida-Pereira et al.Genetics and Molecular Research 16 (2): gmr16029602 studied populations, and most of the genetic diversity was found (87%) within populations. According to the Jaccard's similarity index, none of the studied plants was genetically identical. CTE210 and CTE305 presented high similarity index (0.76), while CTE105 presented low similarity index (<0.16) with all related individuals. ISSR markers were efficient and allowed the formation of a molecular profile, and had sufficient polymorphism to estimate the genetic variability between the accessions of the studied populations.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira¹,

Silva²,

Alves³

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The agro-morphological characterization is the evaluation of all observable traits that could identify varieties or accessions in a collection, and it can be carried out in several forms, depending on the objectives (Moche et al, 2014). Cultivar identification based on phenotypic traits is usually influenced by the environment, which hinders the classification (Aghaei et al, 2012). Conversely, DNA fingerprinting with molecular markers allows precise, objective, and rapid cultivar identification, and has proven to be an efficient tool for germplasm characterization and management (Chakravarthi & Naravaneni, 2006).…”

Section: Palavras-chavementioning

confidence: 99%

“…ISSR markers have higher reproducibility when compared with RAPDs markers (Random Amplified Polymorphic DNA) and are cheaper than the AFLPs markers (Amplified Fragment Length Polymorphism) (Ng & Tan, 2015). This technique has been used to estimate the genetic diversity in different medicinal and aromatic plants, such as Ocimum (Aghaei et al, 2012;Chen et al, 2013;Patel et al, 2015), Varronia curassavica (Brito et al, 2016), and Croton tetradenius (Almeida-Pereira et al, 2016).…”

Section: Palavras-chavementioning

confidence: 99%

Genetic divergence in basil cultivars and hybrids

et al. 2019

View full text Add to dashboard Cite

Basil is an aromatic herb that stands out for its economic importance. It is consumed in natura and used to obtain essential oil. The cultivation of this species in several regions of the world has allowed variations by natural crosses and euploidy, leading to the wide genetic variability found nowadays. Considering the importance of this species, we aimed to analyze the genetic diversity of 27 basil genotypes using ISSR molecular markers. Fourteen primers were employed for DNA amplification, resulting in 86% polymorphism. Based on the Jaccard’s dissimilarity index, the highest index (0.80) was observed between the individuals BAS001 and BAS012, while the lowest index (0.18) was detected between the genotypes BAS014 and BAS015. The genetic similarity among individuals was calculated, forming four distinct clusters. Most individuals (40.7%) were allocated in cluster I. The polymorphic information content (PIC) (0.89) indicated considerable levels of genetic diversity among genotypes. In this sense, the ISSR markers were efficient in the detection of polymorphisms between the accessions, suggesting the genetic variability of the collection. This result demonstrates the importance of the use of molecular markers and the advantages that this information provides to the breeding of the species.

show abstract

“…ISSR markers have been shown to be particularly useful in genetic fingerprinting and diversity analysis (Godwin et al 1997;Aghaei et al 2012). ISSR is an ideal method for fingerprinting and a useful alternative to single-locus or hybridization-based methods because large numbers of DNA fragments are amplified per reaction, representing multiple loci from across the genome (Román et al 2002;Aghaei et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…ISSR is an ideal method for fingerprinting and a useful alternative to single-locus or hybridization-based methods because large numbers of DNA fragments are amplified per reaction, representing multiple loci from across the genome (Román et al 2002;Aghaei et al 2012). Closely related species can be differentiated, as well as individuals within populations of a single species, with ISSR-PCR (Hristova et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of Orobanche aegyptiaca infesting tobacco in Iran by Bayesian analysis

et al. 2014

Self Cite

View full text Add to dashboard Cite

Broomrapes (Orobanche L.) are holoparasitic plants, parasitizing roots of a wide range of host plants. In this study, genetic polymorphism among 44 Orobanche aegyptiaca Pers. individuals collected from different regions of northwest Iran was investigated using inter-simple sequence repeat (ISSR) markers. Two hundred-sixty one discernible bands were amplified using 20 ISSR primers which 245 (94%) was polymorphic, indicating considerable genetic variation among the examined individuals. The number of polymorphic bands per primer ranged from 4 to 17, averaging 12.25. UPGMA clustering using Jaccard's similarity coefficients revealed six main groups. Genetic similarity coefficients varied from 0.71 (between individuals 23 and 27) to 0.34 (between 13 and 30). A model-based Bayesian approach subdivided 38 out of 44 broomrape genotypes into 2 genetic groups and the remaining ones were categorized as mixed genotypes based on Q values. According to an analysis of molecular variance, 99% of the total variation was partitioned within genetic groups. The results demonstrated the potential usefulness of ISSR markers for determination of genetic variation in O. aegyptiaca. Understanding the variability in broomrape is important when attempting to develop resistant host crops against this parasite.

show abstract

Molecular characterisation and similarity relationships among iranian basil (Ocimum basilicum L.) accessions using inter simple sequence repeat markers

Cited by 19 publications

References 14 publications

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Genetic divergence in basil cultivars and hybrids

Genetic variability of Orobanche aegyptiaca infesting tobacco in Iran by Bayesian analysis

Contact Info

Product

Resources

About