Molecular characterisation of Banana mild mosaic virus, a new filamentous virus in Musa spp.

Gambley, C. F.; Thomas, J. E.

doi:10.1007/s007050170097

Cited by 27 publications

(20 citation statements)

References 34 publications

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“…Similar symptoms of chlorotic leaf spotting and fruit deformation are associated with peach infection by Asian prunus viruses (75), while AOPRV is held responsible for a lethal disease of African oil palms (88). None of the putative flexiviruses infecting banana (BanMMV and BanVX) seems to be a serious pathogen (40,113).…”

Section: Gvb: Grapevine Virus Bmentioning

confidence: 99%

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

Martelli¹,

Adams

Kreuze

et al. 2007

Annu. Rev. Phytopathol.

322

196

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The plant virus family Flexiviridae includes the definitive genera Potexvirus, Mandarivirus, Allexivirus, Carlavirus, Foveavirus, Capillovirus, Vitivirus, Trichovirus, the putative genus Citrivirus, and some unassigned species. Its establishment was based on similarities in virion morphology, common features in genome type and organization, and strong phylogenetic relationships between replicational and structural proteins. In this review, we provide a brief account of the main biological and molecular properties of the members of the family, with special emphasis on the relationships within and among the genera. In phylogenetic analyses the potexvirus-like replicases were more closely related to tymoviruses than to carlaviruses. We postulate a common evolutionary ancestor for the family Tymoviridae and the two distinct evolutionary clusters of the Flexiviridae, i.e., a plant virus with a polyadenylated genome, filamentous virions, and a triple gene block of movement proteins. Subsequent recombination and gene loss would then have generated a very diverse group of plant and fungal viruses.

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Section: Gvb: Grapevine Virus Bmentioning

confidence: 99%

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

Martelli¹,

Adams

Kreuze

et al. 2007

Annu. Rev. Phytopathol.

322

196

View full text Add to dashboard Cite

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“…Random RT-PCR was conducted as described by Gambley and Thomas (2001) on purified virus prepared using the modified method of Wakman et al (1995). The heat shock protein 70 homologue (Hsp70h) region of the closterovirid genome was also amplified by RT-PCR using the degenerate primers HSP-P-1 and Tian-P2(M) (Tian et al 1996; water to a final volume of 25 µL.…”

Section: Reverse Transcriptase (Rt)-pcrmentioning

confidence: 99%

The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease

Gambley

Steele²,

Geering³

et al. 2008

Austral. Plant Pathol.

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Abstract. Pineapple mealybug wilt-associated virus 1 (PMWaV-1), 2 (PMWaV-2) and 3 (PMWaV-3) have been detected in Australian commercial pineapple crops, along with a previously undescribed ampelovirus, for which the name Pineapple mealybug wilt-associated virus 5 (PMWaV-5) is proposed. Partial sequences extending from open reading frame 1b through to the heat shock protein homologue were obtained for PMWaV-1, -3 and -5. Phylogenetic analyses of selected regions of these sequences indicated that PMWaV-5 is a distinct species and most closely related to PMWaV-1. The amino acid sequence variation observed in the RNA-dependent RNA polymerase region of PMWaV-1 isolates was 95.8-98.4% and of PMWaV-3 isolates was 92.2-99.5%.In surveys of mealybug wilt disease (MWD) affected crops, none of the four viruses was clearly associated with the disease at all survey sites. A statistically significant association (P < 0.001) between the presence of PMWaV-2 and symptoms was observed at one survey site (site 3), but the virus was at a low incidence at the remaining three survey sites. By contrast, although PMWaV-1 and -3 were equally distributed between symptomless and MWD-affected plants at site 3, there was a statistically significant (P < 0.001) association between each of these two viruses and MWD at sites 1 and 4. At site 2, there was a statistically significant (P < 0.001) association only between PMWaV-3 and MWD. PMWaV-1 was the most commonly found of the four viruses and conversely PMWaV-5 was only occasionally found. Australian isolates of PMWaV-1, -2 and -3 were transmitted by the mealybug species Dysmicoccus brevipes.

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“…AATAAA motif was found completely conserved in two CPMMV isolates from USA (CPMMV-H; AF024628 and CPMMV-M; AF024629; Figure 1) along with 7 HpLV isolates (EF202598, EF202599, EF202600, EF394781, EF394782, EF394783 and EF394784) from China ( Figure 2) and 3 DVS isolates (AJ971469, AJ971470 and AJ971471) from South Korea (Figure 3). This motif is also found in other viruses such as Rice tungro bacilliform virus (RTBV) belonging to caulimoviridae family, where it forms an essential part of the poly (A) signal (Rothnie et al, 2001) and in 3´ untranslated region of ORF5 of Banana mild mosaic virus (genus not assigned) (Gambley and Thomas, 2001). The AATAAA nucleotide motif was absent from multiple sequence alignment of Canada and India.…”

Section: Resultsmentioning

confidence: 89%

Inference on Coat Protein Evolution of Lily Symptomless Carlavirus in India and Abroad Based on Motifs Study and Phylogenetic Analysis

Huddone¹,

Sv²,

Rattan³

et al. 2010

J Proteomics Bioinform

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Lily symptomless carlavirus (LSV), the most common lily infecting virus around the world, contains 6 open reading frames (ORFs) in its genome, of which ORF5 representing coat protein (CP) is the most variable region and is used here to deduce phylogeny of the virus. CP gene of one of the LSV isolates present in the region, LSV isolate-Oh (Accession no. AJ748277) was taken as test sequence.Multiple sequence alignment of test sequence with ClustalW showed nucleotide and amino acid homology of up to 17-98% and 1-98%, respectively with other 78 carlaviral sequences from India and abroad. One conserved nucleotide motif of carlaviruses, AATAAA (Polyadenylation signal motif) was searched for, in the multiple sequence alignments but it was not found in any of the LSV isolates under study. Further, phylogenetic analysis of nucleotide sequences by DNADIST method of Neighbor-joining algorithm placed test LSV isolate most closely to its native LSV isolates from India and, LSV isolates Yunnan and Lanzou, from China. It could be interpreted that in Lily symptomless carlavirus at nucleotide level, evolution is taking place at a faster pace. Also, this virus shared its most recent common ancestry (MRCA), both with its native LSV isolates from India and as well as with LSV isolates from China, probably, indicating its origin from either of the countries. This study provides important clues about spread of the virus and to the best of our knowledge it is the fi rst detailed study of LSV coat protein gene performed at nucleotide level.

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Molecular characterisation of Banana mild mosaic virus, a new filamentous virus in Musa spp.

Cited by 27 publications

References 34 publications

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

FamilyFlexiviridae: A Case Study in Virion and Genome Plasticity

The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease

Inference on Coat Protein Evolution of Lily Symptomless Carlavirus in India and Abroad Based on Motifs Study and Phylogenetic Analysis

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