Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania

Vieira, Patricia Manuela; Mederle, N.; Lobo, Maria; Imre, K.; Mederle, Ovidiu Alexandru; Xiao, Lihua; Dărăbuş, G.; Matos, Olga

doi:10.14411/fp.2015.002

Cited by 27 publications

(21 citation statements)

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“…Molecular characterisation of five isolates indicated the presence of species C. parvum (n = 3) and C. ubiquitum (n = 2) [88]. Vieira et al has also reported the presence of the C. parvum subtype IIdA22G1 in faecal samples of four children under 12 years of age from Timiş County in this area of Romania [89]. …”

Section: Resultsmentioning

confidence: 99%

“…Research focusing on livestock is limited and mostly involves the western [89,92-94], central and north-western [95] regions of the country. The methods applied included non-molecular (conventional acid-fast staining and classical microscopic examination, copro-antigen detection immunoassays) and molecular tools (PCR-restriction fragment length polymorphism (RFLP), DNA sequencing).…”

Section: Resultsmentioning

confidence: 99%

“…are common parasites of domestic animals, including pets, in eastern Europe, and importantly, genotypes pathogenic to humans, including C. parvum and G. intestinalis assemblage A and B , are prevalent. C.parvum subtype IIaA16G1R1 is a common subtype in the region, found in both cattle and humans in the Czech Republic, Estonia, Hungary, Romania and Slovenia [62,69,89,117,119]. It has also been suggested that birds may be carriers of human pathogenic species and genotypes of Giardia and Cryptosporidium [70].…”

Section: Discussionmentioning

confidence: 99%

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Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016

et al. 2018

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IntroductionThis paper reviews the current knowledge and understanding of Cryptosporidium spp. and Giardia spp. in humans, animals and the environment in 10 countries in the eastern part of Europe: Bosnia and Herzegovina, Croatia, Czech Republic, Estonia, Hungary, Latvia, Poland, Romania, Serbia and Slovenia. Methods: Published scientific papers and conference proceedings from the international and local literature, official national health service reports, national databases and doctoral theses in local languages were reviewed to provide an extensive overview on the epidemiology, diagnostics and research on these pathogens, as well as analyse knowledge gaps and areas for further research. Results: Cryptosporidium spp. and Giardia spp. were found to be common in eastern Europe, but the results from different countries are difficult to compare because of variations in reporting practices and detection methodologies used. Conclusion: Upgrading and making the diagnosis/detection procedures more uniform is recommended throughout the region. Public health authorities should actively work towards increasing reporting and standardising reporting practices as these prerequisites for the reported data to be valid and therefore necessary for appropriate control plans.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016

et al. 2018

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“…Among them, calves in most industrialised countries are commonly infected with IIa subtypes, especially subtype IIaA15G2R1 (Xiao, 2010). In addition, IId subtypes have been identified in dairy calves in some countries such as Sweden, Turkey, Romania and Egypt (Bjorkman et al, 2015;Vieira et al, 2015;Ibrahim et al, 2016;Taylan-Ozkan et al, 2016). In contrast, pre-weaned dairy calves in China are commonly infected with C. bovis, and when they are infected with C. parvum, almost exclusively with IId subtypes, especially IIdA15G1 and IIdA19G1 In 2016, a diarrhea outbreak was identified in neonatal dairy calves on a large dairy farm in Jiangsu Province, China.…”

Section: Introductionmentioning

confidence: 99%

Outbreak of cryptosporidiosis due to Cryptosporidium parvum subtype IIdA19G1 in neonatal calves on a dairy farm in China

Wang

Cai

et al. 2019

International Journal for Parasitology

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a b s t r a c tNeonatal diarrhea is one of the most important syndromes in dairy cattle. Among enteropathogens, Cryptosporidium spp. are primary causes of diarrhea, but outbreaks due to cryptosporidiosis are rarely reported in cattle. From January to April in 2016, severe diarrhea was observed in over 400 neonatal dairy calves on a large dairy farm in Jiangsu Province of East China. Approximately 360 calves died due to watery diarrhea despite antibiotic therapy. In this study, 18 fecal specimens were collected from seriously ill calves on this farm during the diarrhea outbreak, and analysed for common enteropathogens by enzymatic immunoassay (EIA). In a post-outbreak investigation, 418 and 1372 specimens collected from animals of various age groups were further analysed for rotavirus and Cryptosporidium spp. by EIA and PCR, respectively, to assess their roles in the occurrence of diarrhea on the farm. Cryptosporidium spp. were genotyped using established techniques. Initial EIA tests showed that 15/18 seriously ill calves during the outbreak were positive for Cryptosporidium parvum, while 8/18 were positive for rotavirus. The overall infection rate of Cryptosporidium in pre-weaned calves on the farm was 22.7%, with odds of the Cryptosporidium infection during the outbreak 4.4-23.5 times higher than after the outbreak. Four Cryptosporidium spp. were identified after the outbreak including C. parvum (n = 79), Cryptosporidium ryanae (n = 48), Cryptosporidium bovis (n = 31), and Cryptosporidium andersoni (n = 3), with co-infections of multiple species being detected in 34 animals. Infection with C. parvum (73/79) was found in the majority of calves aged 3 weeks, consistent with the age of ill calves during the outbreak. All C. parvum isolates were identified as subtype IIdA19G1. In the post-outbreak investigation, C. parvum infection was associated with the occurrence of watery diarrhea in pre-weaned calves, C. ryanae infection was associated with moderate diarrhea in both pre-and post-weaned calves, while no association was identified between rotavirus infection and the occurrence of diarrhea. Results of logistic regression analysis further suggested that C. bovis infection might also be a risk factor for moderate diarrhea in calves. Thus, we believe this is the first report of a major outbreak of severe diarrhea caused by C. parvum IIdA19G1 in dairy calves. More attention should be directed toward preventing the dissemination of this virulent subtype in China.Ó

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“…Inwazje C. parvum u pozostałych gatunków zwierząt gospodarskich i towarzyszących Wprowadzenie metod molekularnych do diagnostyki parazytologicznej pozwoliło na identyfikację C. parvum również u innych gatunków zwierząt niż przeżuwacze. Zarażenia C. parvum w stadach trzody chlewnej są na ogół rzadkie (70,87,107,115), z ekstensywnością inwazji na poziomie stada nie przekraczającą 1,4% (111). Poza Europą wynosi ona od 5,4% (Stany Zjednoczone) do 33,2% w Japonii (5, 40).…”

Section: Ryc 1 Mapa Polski Przedstawiająca Poszczególne Regiony Kraunclassified

Epidemiology of the invasion of Cryptosporidium parvum in farm and wild animals

Kaupke

Rzeżutka

2017

Medycyna Weterynaryjna

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Cryptosporidium parvum is a zoonotic protozoan parasite occurring in a wide range of hosts. Invasions caused by this parasite have been reported in humans and in many animal species including birds. Despite its worldwide prevalence, infections have usually generated considerable losses in the livestock industry, mostly affecting calves, lambs and goat kids. It has previously been shown that ruminants are a major reservoir of zoonotic Cryptosporidium parvum and contact with an infected animal can lead to human infection. The application of molecular methods for parasitological diagnostics has increased our knowledge on the parasite hosts and its prevalence in humans and animals. They also confirmed their usefulness during epidemiological investigations and in surveillance studies of human and animal cryptosporidiosis. In this review the current state of knowledge concerning the importance of Cryptosporidium parvum invasions in farm and wild animals was presented.

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Molecular characterisation of Cryptosporidium (Apicomplexa) in children and cattle in Romania

Cited by 27 publications

References 27 publications

Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016

Review of Cryptosporidium and Giardia in the eastern part of Europe, 2016

Outbreak of cryptosporidiosis due to Cryptosporidium parvum subtype IIdA19G1 in neonatal calves on a dairy farm in China

Epidemiology of the invasion of Cryptosporidium parvum in farm and wild animals

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