Molecular characterisation of Giardia duodenalis from human and companion animal sources in the United Kingdom using an improved triosephosphate isomerase molecular marker

Krumrie, Sarah; Capewell, Paul; McDonald, Mike; Dunbar, Dawn; Panarese, Rossella; Katzer, Frank; Sakka, Noha El; Mellor, D. J.; Alexander, Claire L.; Weir, William

doi:10.1016/j.crpvbd.2022.100105

Cited by 5 publications

(5 citation statements)

References 67 publications

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“…However, this potential association needs to be further investigated. Our results are in line with previous findings at the regional level [12], but they are divergent from those of other studies performed in Spain and the UK, which indicated that assemblage B was more frequent [29][30][31]. A recent review [5] concluded that assemblage B was the most frequently identified both at the European and world-wide level.…”

Section: Discussionsupporting

confidence: 60%

Prevalence and Genotyping of Water- and Food-Borne Parasitic Protozoans (Giardia duodenalis and Cryptosporidium spp.) in Hospitalized Patients from Northwestern Romania

Ionică,

Ieremia,

Kalmár

et al. 2024

Microorganisms

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Giardia duodenalis and Cryptosporidium spp. are important zoonotic protozoan pathogens that infect the gastro-intestinal tract of numerous vertebrates, including humans, and both parasites are responsible for water- or food-borne outbreaks of disease worldwide. Although, globally, both parasites are highly prevalent, particularly in developing countries, epidemiological data from Romania are scarce, and genotyping has rarely been performed. The aims of the present study were to investigate the occurrence and genetic diversity of G. duodenalis and Cryptosporidium spp. in patients hospitalized in Northwestern Romania in relation to clinical and paraclinical presentation and to identify the relative frequency of non-specific symptoms and potential risk factors. Between June 2022 and January 2024, 426 fecal samples were screened for gastro-intestinal parasites by rapid tests and microscopical examination, further confirmed by PCR and sequencing. Giardia duodenalis was detected and characterized in 12 samples (2.82%), while Cryptosporidium parvum was confirmed in four samples (0.94%). A majority of positive patients were symptomatic and reported nausea and vomiting with a significantly higher frequency compared to negative ones. This study provides new insights into the epidemiological status and clinical implications of gastro-intestinal parasite species and genospecies in Romania that are necessary for an in-depth understanding of the potential zoonotic transmission and improvement of patient care.

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Section: Discussionsupporting

confidence: 60%

Prevalence and Genotyping of Water- and Food-Borne Parasitic Protozoans (Giardia duodenalis and Cryptosporidium spp.) in Hospitalized Patients from Northwestern Romania

Ionică,

Ieremia,

Kalmár

et al. 2024

Microorganisms

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“…This agreed with the low eradication rate for these two regimens in Egyptian clinical trials (Diab et al, 2018). Also, Liou et al (2013) reported that H. pylori may be associated with extraintestinal diseases, including immune thrombocytopenic purpura, refractory iron deficiency anemia, and vitamin B12 deficiency. Hassan et al (2023) in Egypt reported that combined pre-biotic and probiotic supplementation gave promising anti-Giardia activity and restored the intestinal structures and modulate IgA response, apart from giving synergistic effects when added to Nitazoxanide.…”

Section: Introductionsupporting

confidence: 58%

Zoonotic Giardiasis and Its Complications: A Review Article

Morsy

Abou-Elmagd

Mousa

2023

Journal of the Egyptian Society of Parasitology

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Giardiasis is a protozoan flagellated zoonotic parasite, causing diarrheal disease caused by G. duodenalis person or animal has been infected with Giardia, the parasite lives in the intestines and is passed in stool (poop). It causes mild or severe diarrhea, gas, stomach cramps, nausea (a feeling of stomach upset), or dehydration (body water loss causes weakness of dizziness). Some people experience no symptoms at all. Cysts once pass are infective, spreads easily and can spread from person to person or through contaminated water, food, surfaces, or objects. Giardiasis shares some pathogens some or less the clinical manifestations.

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“…This study also identified a few assemblage AI in both human and dog samples. [ 21 ] In another recent study from the UK,[ 28 ] 17.4% (8/46) of feline-derived Giardia strains, from companion animals, were potentially zoonotic, namely assemblages B and AI.…”

Section: Discussionmentioning

confidence: 99%

Molecular appraisal of Giardia intestinalis from Western India: A prospective observational study

Mohammad,

Tak,

Bohra

et al. 2024

Tropical Parasitology

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Background: Giardia intestinalis is an intestinal protozoan which commonly causes parasitic gastroenteritis globally. It is a species complex consisting of at least eight assemblages (genotypes). In India, Giardia is mostly underreported and missed in asymptomatic cases. Aim: The aim of this study was to genotype the G. intestinalis isolates from stool samples of patients at a tertiary care center in Rajasthan, India, and to clinically correlate it. Methods: This prospective pilot cross-sectional study was conducted from 2019 to 2021 in a tertiary care center in western India. Patients who were microscopically positive for giardiasis were enrolled. DNA was extracted from their stool samples and amplified by polymerase chain reaction (PCR) using 4E1-HP as the target sequence. Anthropometric measurements and analysis were done for children by using Anthrocal application. Results: A total of 50 patients were enrolled. Diarrhea was present in 18 patients (36%). Among these, 6 were immunocompromised and had different comorbidities. Among the children <12 years of age, 55.17% (n = 16/29) were stunted (<−2 S.D.), and among <5 years, 44.4% (n = 4/9) showed wasting (<−2 S.D.). A PCR product corresponding to assemblage B of G. intestinalis was amplified in 47 stool specimens. Only three stool samples were negative for both assemblages A and B and posed an interesting enigma. Conclusion: In this study, a predominance of assemblage B of G. intestinalis was detected in 94% of the isolates. Furthermore, the possibility of zoonotic transmission could not be ruled out.

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Molecular characterisation of Giardia duodenalis from human and companion animal sources in the United Kingdom using an improved triosephosphate isomerase molecular marker

Cited by 5 publications

References 67 publications

Prevalence and Genotyping of Water- and Food-Borne Parasitic Protozoans (Giardia duodenalis and Cryptosporidium spp.) in Hospitalized Patients from Northwestern Romania

Prevalence and Genotyping of Water- and Food-Borne Parasitic Protozoans (Giardia duodenalis and Cryptosporidium spp.) in Hospitalized Patients from Northwestern Romania

Zoonotic Giardiasis and Its Complications: A Review Article

Molecular appraisal of Giardia intestinalis from Western India: A prospective observational study

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