Molecular characterisation of multidrug-resistant  Bacteroides  isolates from Hungarian clinical samples

Sárvári, Károly Péter; Sóki, Jozsef; Kristóf, Katalin; Juhász, Emese; Miszti, Cecília; Melegh, Szilvia; Latkóczy, Krisztina; Urbán, Edit

doi:10.1016/j.jgar.2017.10.020

Cited by 19 publications

(9 citation statements)

References 18 publications

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“…[1][2][3] In recent years, MDR Bacteroides fragilis (B. fragilis) has emerged in several areas around the world. [4][5][6] However, its resistance capability has been significantly varied among different geographical locations. 7 In treating an infection of Bacteroides spp., metronidazole, clindamycin and β-lactam antimicrobials are routinely prescribed.…”

Section: Introductionmentioning

confidence: 99%

Higher Prevalence of Multi-Antimicrobial Resistant Bacteroides spp. Strains Isolated at a Tertiary Teaching Hospital in China

Wang

Han

Shen

et al. 2020

IDR

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The study investigates the molecular epidemiology of multi-drug resistant (MDR) Bacteroides spp. isolates and the clinical characteristics of the patients. Materials and Methods: Bacteroides spp. clinical strains were identified through MALDI-TOF MS and VITEK-2 anaerobes and corynebacterium (ANC) cards. A broth microdilution method was employed to detect the antimicrobial sensitivities of Bacteroides spp. isolates. PCR was used to detect the resistance genes, including cfxA, cepA, cfiA, ermF, nim, as well as the upstream insertion sequence (IS) element of the cfiA gene. The effects of broad-spectrum efflux pump inhibitors (EPIs) on the minimal inhibitory concentration (MICs) of cefoxitin, moxifloxacin, and imipenem for MDR Bacteroides spp. were investigated. Results: The total resistance rates of 115 Bacteroides spp. isolates to cefoxitin, moxifloxacin, clindamycin, metronidazole, imipenem and meropenem were 4.3%, 16.5%, 80.0%, 5.2%, 13.9% and 13.9%, respectively. The positive rates of carbapenem resistance gene cfiA were 38.9% and 8.6% for B. fragilis and non-B. fragilis isolates, respectively. The isolation rate of MDR isolates reached up to 18.26% (21/115), and the isolation rate among the gastrointestinal cancer patients was significantly higher when compared to the nongastrointestinal cancer patients (52.38%/26.08%, P = 0.006). Furthermore, MDR isolates were more likely to be isolated from the patients exposed to cephalosporins 3 months before Bacteroides spp. isolation (76.19%/31.52%, P = 0.000). Conclusion:The overall resistance rates of Bacteroides spp. isolates against multiple antimicrobials were at a high level, especially for B. fragilis. The CfiA gene carrying rate among B. fragilis isolates was as high as 38.9%, and its mediated carbapenem resistance was the major resistance mechanism for B. fragilis. The findings of this study imply that the real resistance tendency of Bacteroides spp. may be underestimated and need to be given more attention.

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Section: Introductionmentioning

confidence: 99%

Higher Prevalence of Multi-Antimicrobial Resistant Bacteroides spp. Strains Isolated at a Tertiary Teaching Hospital in China

Wang

Han

Shen

et al. 2020

IDR

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“…Total DNA of the isolates were extracted by bacterial DNA extraction kit (Qiagen Ltd, Germany) and the most common antibiotic resistance genes (cepA, cfxA, cfiA, ermF, nim) [13] and the insertion sequence (IS) upstream [4] of the cfiA gene was amplified. Then the PCR products were analysed with 1.2% agarose gel electrophoresis, and the DNA amplicon of the PCR was purified using the QIA quick Gel Extraction Kit (Qiagen Ltd, Germany).…”

Section: Detection Of Resistance Genesmentioning

confidence: 99%

“…is a kind of common anaerobes inhabiting the intestines of humans and is the most common anaerobes recovered from various infections, such as intra-abdominal infection, foot ulcer and bloodstream infection [1,2,3]. In recent years, multi-drug resistant Bacteroides fragilis emerged in several areas around the world [4,5,6], but resistance features varied greatly among different areas [7].…”

Section: Introductionmentioning

confidence: 99%

Higher prevalence of multi-antimicrobial resistant Bacteroides spp. strains isolated at a tertiary teaching hospital, China

Wang¹,

Han²,

Shen³

et al. 2019

Preprint

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Background: The antimicrobial resistance of Bacteroides spp. isolates is reported to be increasing among different districts around the world, and few reports found that multi-antimicrobial resistant strains emerged. However, less is known about the prevalence of multi-antimicrobial resistant isolates in China, especially for carbapenem resistance. Methods: Bacteroides spp. clinical strains were isolated from inpatients at a 3000 bed tertiary teaching hospital , and were identified by MALDI-TOF MS and VITEK-2 anaerobes and corynebacterium (ANC) card. Broth microdilution method was employed to detect the antimicrobial sensitivities of Bacteroides spp. isolates and PCR method was used to detect the resistance genes, including cfxA, cepA, cfiA, ermF and nim. The upstream insertion sequence (IS) element of cfiA gene was further detected and verified. Results: Among 115 Bacteroides spp. strains enrolled in this study, 80 isolates were Bacteroides fragilis and 35 isolates were non-Bacteroides fragilis. The total resistance rates of 115 Bacteroides spp. isolates to ampicillin/sulbactam, amoxicillin/clavulanic, cefoxitin, piperacillin, piperacillin/tazobactam, moxifloxacin, clindamycin, metronidazole, imipenem and meropenem were 22.6%, 19.6%, 3.5%, 27.8%, 8.7%, 16.5%, 80.0%, 5.2%, 13.9% and 13.9% , respectively. Except cefoxitin and moxifloxacin, the resistance rates of B. fragilis isolates to the above antibiotics were all higher than those of non-B. fragilis isolates. The positive rates of carbapenem resistance gene cfiA were 38.9% and 8.6% for B. fragilis and non-B. fragilis isolates, respectively. For 15 carbapenem resistant B. fragilis isolates, the co-carrying rates of carbapenem resistance gene cfiA and its upstream IS element were 86.7% (13/15). Conclusions: The overall resistance rates of Bacteroides spp. isolates toward multiple antibiotics were at a higher level, especially for B. fragilis. CfiA gene carrying rate among B. fragilis isolates was high up to 38.9% and its mediated carbapenem resistance was a major resistance mechanism for B. fragilis. The findings of this study imply that the actual resistance tendency of Bacteroides spp. may be underestimated and need to be given more attention to.

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“…Two strains were from the EICU, obstetrics and gynecology, ICU and general surgery, and each department had one strain. The prevalence of MDR B. fragilis is considered to be associated with resistance genes and the abuse of antibiotics (22).…”

Section: Gram Positive Bacteriamentioning

confidence: 99%

Identification and Antimicrobial Susceptibility of Clinically Isolated Anaerobic Bacteria: A Retrospectively Study in a Jiangxi Tertiary-Care Hospital

Zou

Xiao-ying

Zeng

et al. 2019

Jundishapur J Microbiol

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Background: Anaerobic infections have been reported for many years, and there is an increasing trend in these infections worldwide, but anaerobic infections have not received sufficient attention. Rapid identification is important for the treatment of anaerobes because of their different antibiotic-resistance profiles. Objectives: This study aimed to analyze the hospital's present condition to improve anaerobic culture detection rates and enhance the monitoring of anaerobes in hospitals. Methods: This study retrospectively analyzed sterile body fluids sent to the First Affiliated Hospital of Nanchang University in the form of culture bottles in 2017. Finally, 28 strains of obligate anaerobes were isolated, then combined 16S rRNA gene sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to identify the strains and performed separate antimicrobial susceptibility testing. Results: The results showed that these two methods are highly consistent. There were 17/28 (61%) Gram-negative and 11/28 (39%) Gram-positive bacteria. The predominant bacteria were Bacteroides fragilis (15/28). Ten strains were isolated from the Obstetrics and Gynecology Department. The next most frequently affected departments were General Surgery (17.86%) and the ICU (17.86%). We analyzed the resistance to penicillin, cefoxitin, clindamycin, metronidazole, meropenem, piperacillin/tazobactam and amoxicillin/clavulanic acid by using the agar dilution method. The resistance rates to clindamycin were relatively high but relatively sensitive to metronidazole. Conclusions: The results of this research indicate that we should pay attention to the cultivation of anaerobic bacteria, especially in certain high-risk departments.

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Molecular characterisation of multidrug-resistant Bacteroides isolates from Hungarian clinical samples

Cited by 19 publications

References 18 publications

<p>Higher Prevalence of Multi-Antimicrobial Resistant <em>Bacteroides</em> spp. Strains Isolated at a Tertiary Teaching Hospital in China</p>

<p>Higher Prevalence of Multi-Antimicrobial Resistant <em>Bacteroides</em> spp. Strains Isolated at a Tertiary Teaching Hospital in China</p>

Higher prevalence of multi-antimicrobial resistant Bacteroides spp. strains isolated at a tertiary teaching hospital, China

Identification and Antimicrobial Susceptibility of Clinically Isolated Anaerobic Bacteria: A Retrospectively Study in a Jiangxi Tertiary-Care Hospital

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