Molecular characterisation of Mycobacterium bovis isolated from cattle slaughtered at the Bamako abattoir in Mali

Müller, Bjorna; Steiner, Benjamin; Bonfoh, Bassirou; Fané, Adama; Smith, Noel H.; Zinsstag, Jakob

doi:10.1186/1746-6148-4-26

Cited by 70 publications

(74 citation statements)

References 31 publications

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“…Bovine TB is therefore endemic in Cameroon since the cattle slaughtered in these abattoirs are from the main cattle-producing regions of the country including both highland regions in this study. Compared to data recorded elsewhere in Africa, the rate recorded in this study was higher than 0.052% reported by Shitaye et al (2006), but lower than other findings ranging from 0.49% to 11.3% (Du-Sai and Abdullahi 1994;Ankugah 2002;Ameni and Wudie 2003;Asseged et al 2004;Diguimbaye-Djaibe et al 2006;Muller et al 2008;Biffa et al 2009;Cadmus and Adesokan 2009;Ngandolo et al 2009). These differences could be explained by many factors including differences in the disease status in the animal populations, the degree of implemention of the disease control programme and various environmental influences.…”

Section: Prevalence Of Bovine Tuberculosis Based On the Detection Of contrasting

confidence: 54%

Prevalence of bovine tuberculosis in cattle in the highlands of Cameroon based on the detection of lesions in slaughtered cattle and tuberculin skin tests of live cattle

Awah-Ndukum¹,

Kudi²,

Bradley³

et al. 2012

Vet. Med. - Czech

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Bovine tuberculosis (TB) is an important neglected zoonosis in Cameroon, where many communities depend on their livestock for livelihood and the incidence of human TB and TB-HIV/AIDS co-infection are high and increasing annually. The aim of this study was to estimate the prevalence of bovine TB in cattle in the highlands of Cameroon. The magnitude and trend of detecting TB lesions in slaughtered cattle (1994 to 2010) and tuberculin skin tests (TST) in 2853 cattle (84 herds) of 39 livestock rearing communities were analysed. Of 129 165 slaughtered cattle inspected, 599 (0.46%; 95% CI: 0.43%-0.50%) showed suspected TB lesions among a total of 983 (0.76%; 95% CI: 0.71%-0.81%) identified pathologies. The monthly TB detection rates ranged from 0.30% (95% CI: 0.20%-0.40%) to 0.81% (95% CI: 0.64%-0.98%) and annual rates from 0.04% (95% CI: 0%-0.11%) to 1.46% (95% CI: 1.22%-1.69%). The rates were not affected (P < 0.05) by season and fluctuating peaks were also recorded. The comparative TST revealed that bovine TB was widely distributed in live cattle (4.67%; 95% CI: 3.89%-5.44%) and was higher (χ 2 = 17.50, P ≤ 0.001) in the Western highlands than Adamawa plateaux.Comparative TST bovine TB reactors were higher (P < 0.05) in cattle managed in semi-intensive and beef production systems compared to the others. Animals in small herds showed higher (χ 2 = 4.283, P = 0.038) rates than those in large herds. Bovine TB prevalence in exotic/upgraded cattle was comparable to that of the Red Bororo zebu but higher than the rates in Guadali (χ 2 = 4.971, P = 0.026) and White Fulani (χ 2 = 5.6, P = 0.018) zebus.Among the indigenous zebus, the rate was higher in Red Bororo than the Guadali (χ 2 = 6.244, P = 0.012) and White Fulani (χ 2 = 6.568, P = 0.010). Sex did not influence (χ 2 = 0.410, P = 0.522) bovine TB prevalence in this study but diagnosis of the disease was higher (χ 2 = 5.787; P = 0.016) among adult/older cattle than in younger animals. Further analysis of the TST responses revealed that atypical mycobacterial infections was widespread and 6.83% of tested animals showed positive reactions at both bovine and avian tuberculin injection sites and a strong association (χ 2 = 2.512; P = 0.113) between skin responses to both tuberculins. The study confirms that bovine TB is prevalent in live cattle and meat production abattoirs in Cameroon and we recommend strict a interpretation of TST results for maximum diagnosis of the disease in the local environment. A need for comprehensive investigation of the molecular epidemiology, zoonotic risks and the public health importance of bovine TB in Cameroon cannot be overemphasised.

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Section: Prevalence Of Bovine Tuberculosis Based On the Detection Of contrasting

confidence: 54%

Prevalence of bovine tuberculosis in cattle in the highlands of Cameroon based on the detection of lesions in slaughtered cattle and tuberculin skin tests of live cattle

Awah-Ndukum¹,

Kudi²,

Bradley³

et al. 2012

Vet. Med. - Czech

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“…Furthermore, spoligotype profiles of some M. bovis strains isolated in Nigeria had been previously reported by earlier studies in Cameroon (Njanpop-Lafourcade et al, 2001). In Mali, a strain with a similar spoligopattern to SB0944, reported previously in Nigeria, Chad and Cameroon was also observed in 13 of the 20 strains studied (Muller et al, 2008). This therefore gives strong indications regarding clonal distribution of M. bovis strains in West Africa (Muller et al, 2009), and further emphasizes the relevance of molecular techniques in the epidemiology of tuberculosis.…”

Section: Introductionmentioning

confidence: 49%

“…The two human M. bovis isolates (hb74 and NB83) had clearly distinct spoligopatterns and MLVA genotypes, suggesting that they originated from different sources. The spoligotype of strain NB83 is identical to SB1432 in the www.mbovis.org database whilst hb74 has spoligotype SB0944 which has been previously reported in cattle in Nigeria (Cadmus et al, 2006), Chad, Cameroon (Njanpop-Lafourcade et al, 2001, Mali (Muller et al, 2008), France ) and the USA (Driscoll et al, 1999). A similar spoligotype lacking spacer 30 was also reported from a human with pulmonary BTB in Ghana (Meyer et al, 2008).…”

Section: Discussionmentioning

confidence: 90%

Molecular epidemiology of human and animal tuberculosis in Ibadan, Southwestern Nigeria

Jenkins¹,

Cadmus²,

Venter³

et al. 2011

Veterinary Microbiology

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From 2005 to 2007, Mycobacterium tuberculosis complex (MTC) strains were isolated from cattle, goats and pigs samples collected at the Bodija abattoir and from human samples from tuberculosis patients and livestock traders at the Akinyele cattle market in Ibadan, Southwestern Nigeria. Seventy four isolates obtained from humans (24) and livestock (50) were identified as MTC strains. Thirty two isolates were spoligotyped. Nineteen of these 32 isolates were identified as M. tuberculosis whilst 13 were identified as Mycobacterium bovis. M. bovis was isolated from two humans, whereas M. tuberculosis was isolated from a bovine, a pig and a goat. All the M. bovis isolates identified in this study belonged to the Africa 1 clonal complex. Multiple locus VNTR [variable number of tandem repeats] analysis (MLVA) was carried out on the 74 isolates. Three major clusters were defined. Group A consisted of 24 M. tuberculosis isolates (MLVA genotypes 1-18). One strain was isolated from a bovine and one from a pig. Group B consisted of 49 M. bovis strains (MLVA genotypes 19-48), mainly of cattle origin but also included four goat, nine pig and two human isolates. Group C consisted of a single M. tuberculosis isolate (MLVA genotype 49) obtained from a goat. Spoligotyping and MLVA confirmed it as clustering with the East Africa Indian clade found in humans in Sudan and the Republic of Djibouti. The isolation of three M. tuberculosis strains from livestock raises the question of their epidemiological importance as a source of infection for humans.

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“…In Ethiopia, 84% of visible lesions were found in the lungs and thoracic lymph nodes (Teklul et al, 2004). In Mali, 79% of the animals had confirmed lesions in lungs, and the infection with M. bovis was highly associated with these lesions (p < 0.001) (Müller et al, 2008). However, in Tanzania, visible lesions were mainly found in the gastrointestinal tract (61.3%) (Cleaveland et al, 2007); these differences could be attributed to the breeds involved in the studies.…”

Section: Discussionmentioning

confidence: 99%

Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador

Proaño-Pérez

Benítez‐Ortiz

Desmecht

et al. 2011

Preventive Veterinary Medicine

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a b s t r a c tVeterinary inspection in slaughterhouses allows for the detection of macroscopic lesions reminiscent of bovine tuberculosis, but the presence of Mycobacterium bovis must be confirmed by laboratory methods. This study aimed at comparing the performances of the standard diagnostic tools used to identify M. bovis in tissue specimens sampled from suspicious animals. During a two years period, 1390 cattle were inspected at the Machachi abattoir in the Mejia canton -Ecuador. A total of 33 animals with granulomatous lesions were detected, representing 2.33% (16/687) and 2.42% (17/703) animals examined in 2007 and 2008, respectively. Ninety-four tissue specimens were sampled and screened for the presence of mycobacteria. Acid-fast bacilli were identified in one third of the suspicious cattle (11/33) and suggestive microscopic lesions in 27.3% (9/33) of the samples examined by direct microscopy and histopathology, respectively. Culturing on Stonebrink medium and 16S-rRNA-based polymerase chain reaction (PCR) yielded 36.4% (12/33) and 27.3% (9/33) of positives, respectively. Compared to culture, other diagnostic procedures displayed a lower sensitivity, with 56.5% for PCR, and 43.5% for direct microscopy and histopathology; however, the specificity was higher (94.4% for PCR and microscopy, and 97.2% for histopathology). We conclude that reliable post-mortem laboratory testing either requires the combination of a set of available diagnostic tools or necessitates the development of improved new-generation tools with better sensitivity and specificity characteristics.

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Molecular characterisation of Mycobacterium bovis isolated from cattle slaughtered at the Bamako abattoir in Mali

Cited by 70 publications

References 31 publications

Prevalence of bovine tuberculosis in cattle in the highlands of Cameroon based on the detection of lesions in slaughtered cattle and tuberculin skin tests of live cattle

Prevalence of bovine tuberculosis in cattle in the highlands of Cameroon based on the detection of lesions in slaughtered cattle and tuberculin skin tests of live cattle

Molecular epidemiology of human and animal tuberculosis in Ibadan, Southwestern Nigeria

Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador

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