Molecular characterisation of quinolone-resistant Shigella strains isolated in Tehran, Iran

Ranjbar, Reza; Behnood, Vahid; Memariani, Hamed; Najafi, Ali; Mirab-balou, Majid; Mammina, Caterina

doi:10.1016/j.jgar.2016.01.010

Cited by 25 publications

(15 citation statements)

References 27 publications

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“…In two reports from Tehran and Tabriz, Iran, 17.4% and 31% of Shigella spp. isolates were resistant to nalidixic acid, respectively 11,36. In China, Pu et al37 found that 5 (33.3%) of 15 fluoroquinolone-resistant isolates contained the qnrS gene.…”

Section: Discussionmentioning

confidence: 99%

Multidrug-resistant Shigella infection in pediatric patients with diarrhea from central Iran

Abbasi

Abtahi

Belkum

et al. 2019

IDR

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Background: Shigella spp. are primary pathogens of diarrhea in children worldwide. Emergence of resistance to fluoroquinolones and third-generation cephalosporins is crucial in the management of pediatric shigellosis. We determined the prevalence and the antibiotic resistance patterns of Shigella species isolated from pediatric patients in central Iran. Materials and methods: Pediatric diarrhea samples (n=230) were cultured on MacConkey and XLD agar media and in GN broth. Genus-specific PCR for ipaH was also used for detection directly from fecal specimens. Antibiotic resistance and the frequency of ESBL and AmpC genes were determined. Results: Out of the 230 samples, 19 (8.2%) cases of Shigella spp. were identified using culture. Twenty-six samples were positive by PCR (11.3%), S. flexneri (4/19; 21%) and S. sonnei (15/19; 78.9%) being the most detected. The highest antibiotic resistance rates were found for cotrimoxazole (19/19; 100%), ampicillin (16/19; 84.2%), cefixime (13/19; 68.4%) and ceftriaxone (12/19; 63.1%). Ten cases showed phenotypic ESBL presence and all these strains were positive for bla TEM , bla CTX-M-1 , and bla CTX-M-15 . Three strains were AmpC positive, all of which harbored bla CMY-2 and two contained bla CIT . Of the 19 Shigella isolates 5 (26.3%), 2 (10.5%), and 1 (5.2%) were phenotypically resistant to nalidixic acid, ciprofloxacin, and norfloxacin, respectively. Class 1 integron was found in 18 (94.7%) isolates whereas class 2 integron was found in 19 (100%) strains. Conclusion: We found a considerable presence of Shigella species with elevated antibiotic resistance levels. In particular, the resistance to third-generation cephalosporins (ESBL) and ciprofloxacin must be taken seriously.

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Section: Discussionmentioning

confidence: 99%

Multidrug-resistant Shigella infection in pediatric patients with diarrhea from central Iran

Abbasi

Abtahi

Belkum

et al. 2019

IDR

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“…The genus Shigella is divided into four serogroups— S. dysenteriae (serogroup A), S. flexneri (serogroup B), S. boydii (serogroup C), and S. sonnei (serogroup D) [3]. Shigellosis is an invasive illness of the human colon that leads to varied clinical symptoms ranging from mild watery diarrhea to severe colitis [4].…”

Section: Introductionmentioning

confidence: 99%

Profiling of Virulence-associated Factors in Shigella Species Isolated from Acute Pediatric Diarrheal Samples in Tehran, Iran

Yaghoubi

Ranjbar

Dallal

et al. 2017

Osong Public Health Res Perspect

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ObjectivesThe genus Shigella comprises the most infectious and diarrheagenic bacteria causing severe diseases, mostly in children under five years of age. This study aimed to detect nine virulence genes (ipaBCD, VirA, sen, set1A, set1B, ial, ipaH, stx, and sat) in Shigella species (spp.) using multiplex polymerase chain reaction (MPCR) and to determine the relation of Shigella spp. from pediatric diarrheal samples with hospitalization and bloody diarrhea in Tehran, Iran.MethodsShigella spp. were isolated and identified using standard microbiological and serological methods. The virulence genes were detected using MPCR.ResultsSeventy-five Shigella spp. (40 S. sonnei, 33 S. flexneri, 1 S. dysenteriae, and 1 S. boydii) were isolated in this study. The prevalence of ial, sen, sat, set1A, and set1B was 74.7%, 45.4%, 28%, 24%, and 24%, respectively. All S. flexneri isolates, while no S. sonnei, S. dysenteriae, or S. boydii isolates, contained sat, set1A, and set1B. All isolates were positive for ipaH, ipaBCD, and virA, while one (1.4%) of the isolates contained stx. The highest prevalence of virulence determinants was found in S. flexneri serotype IIa. Nineteen (57.6%) of 33 S. flexneri isolates were positive for ipaBCD, ipaH, virA, ial, and sat. The sen determinants were found to be statistically significantly associated with hospitalization and bloody diarrhea (p = 0.001).ConclusionThis study revealed a high prevalence of enterotoxin genes in S. flexneri, especially in serotype 2a, and has presented relations between a few clinical features of shigellosis and numerous virulence determinants of clinical isolates of Shigella spp.

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“…Rapid detection and molecular epidemiology investigation of resistance genes in bacteria are extremely important [ 14 - 17 ].…”

Section: Introductionmentioning

confidence: 99%

The Molecular Study of Antibiotic Resistance to Quinolones in Salmonella enterica Strains Isolated in Tehran, Iran

Malehmir¹,

Ranjbar²,

Harzandi³

2017

TOMICROJ

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Introduction: Salmonella is known as one of the most important causes of gastrointestinal disease in the world. Quinolones and fluoroquinolones are used successfully in the treatment of salmonellosis particularly for infections that have become resistant to several antibiotics. But non-susceptible isolates to quinolones have been reported in several countries. The data are limited about the prevalence of quinolone-resistant isolates in our country. Therefore, this study investigated the plasmid-mediated quinolone resistance genes in Salmonella enterica isolated in Children's Medical Center in Tehran during 2014-2015.Methods and Materials: Salmonella isolates were isolated and identified using standard microbiological methods. Antibiotic susceptibility testing and screening of Salmonella strains resistant to quinolones were performed according to the CLSI guidelines. The molecular investigation was done using specific primers for detection of qnr genes including: qnrA, qnrB and qnrS, by polymerase chain reaction.Results:Overall, 92 (66.6%) strains were resistant to nalidixic acid. None of the strains showed resistance to ciprofloxacin. Out of the 92 nalidixic acid resistant strains, 52 (56.52%) harbored qnrS genes, 15 strains (16.30%) had both qnrA and qnrS genes. Two (1.1%) isolates were positive for qnrB gene. Twenty four (26.08%) nalidixic acid resistant isolates did not have any qnr qens.Conclusion:The results of this study show high prevalence of resistance to nalidixic and qnr genes in Salmonella isolates. Plasmid nature of this type of resistance poses an increased risk of dissemination of quinolone resistance between Salmonella and non-Salmonella isolates circulating in hospitals environments.

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Molecular characterisation of quinolone-resistant Shigella strains isolated in Tehran, Iran

Cited by 25 publications

References 27 publications

<p>Multidrug-resistant <em>Shigella</em> infection in pediatric patients with diarrhea from central Iran</p>

<p>Multidrug-resistant <em>Shigella</em> infection in pediatric patients with diarrhea from central Iran</p>

Profiling of Virulence-associated Factors in Shigella Species Isolated from Acute Pediatric Diarrheal Samples in Tehran, Iran

The Molecular Study of Antibiotic Resistance to Quinolones in Salmonella enterica Strains Isolated in Tehran, Iran

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