Molecular Characterisation of the Faecal Microbiota in Patients with Type II Diabetes

Wu, Xiaokang; Ma, Chaofeng; Han, Lihua; Nawaz, Muhammad; Gao, Fei; Zhang, Xuyan; Yu, Pengbo; Zhao, Chuanqi; Li, Lianchuan; Zhou, Aiping; Wang, Juan; Moore, John E.; Millar, B. Cherie; Xu, Jiru

doi:10.1007/s00284-010-9582-9

Cited by 408 publications

(281 citation statements)

References 41 publications

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“…For amplification of the V1-V4 regions of the 16S rRNA gene, 100 ng of DNA was used on a Genome Sequencer FLX Titanium platform. Sequencing reads were quality filtered, used and subject to Operational Taxonomic Unit (OTU) analysis clustered identified using QIIME software [13] and RDP classifier [14]. The representative sequence from each OTU were blasted to the database of the Ribosomal Database Project (RDP) and aligned.…”

Section: S Rrna Gene Sequence Analysismentioning

confidence: 99%

Food Additive P-80 Impacts Mouse Gut Microbiota Promoting Intestinal Inflammation, Obesity and Liver Dysfunction

Singh¹

2016

SOJMID

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Open Access Research Articlethe globalization of unhealthy lifestyle. Key metabolic and physiological changes, including obesity, metabolic syndrome and Nonalcoholic Fatty Liver Disease (NAFLD) are commonly associated with chronic, non-communicable disease that are responsible for approximately 70% of the world's death.There are compelling arguments to include the composition of gut microbiota as a key risk factor for the development of obesity and obesity related metabolic disorders, including insulin resistance, type 2 diabetes, dyslipidemia and non-alcoholic fatty liver disease [3]. The gut microbiota has been implicated in having both direct (i.e. through effect on nutrient uptake) and indirect effect on health [4,5]. In mice, food additives have been demonstrated to affect the host microbiota interaction, leading to low-grade inflammation, adiposity and adiposity associated metabolic effect [6].We set out to elucidate whether alteration in the gut microbial community after P-80 ingestion is a causative agent of liver dysfunction. To explore this possibility, we setup an experiment to examine the impact of P-80 on gut microbiota using wild-type C57BL/ 6 mice. The experimental mice were gavaged with the amount of P-80 equal to 1% of their body weight. This amount correlates to the acceptable daily human intake of 1% per kg of polysorbate-80 to body weight [7]. Methods Mice and dietThe C57BL/6 (000664) mice were purchased from The Jackson Laboratory. The mice were maintained under specific pathogenfree conditions, on a 12-h light-dark cycle, and fed normal diet (12 kcal% fat, 29 kcal% protein, 59% kcal carbohydrate) ad libitum. For this study there were two groups: one control group (ingesting saline via gavage) and one experimental P-80 fed group (ingesting P-80 dissolve in saline, 1% per kg via gavage). This dose corresponds to the FDA Acceptable Daily Intake (ADI) in humans (1% per kg (body weight), adjusted to the mouse AbstractThe increasing prevalence of obesity has emerged as one of the most important global public health issue. The change to the human microbiome as a result of changes in the quality and quantity of food intake over the past several decades has been implicated in the development of obesity and metabolic syndrome. We administered polysorbate-80 to mice via gavage. The researchers monitor liver noninvasively using a bioluminescence imaging. For the liver dysfunction we measure the liver enzymes and PAS stain on liver, electron microscopy liver mitochondria. For the assessment of intestinal inflammation we measured fecal LCN2, LPS, MPO and flagellin by ELISA and qPCR. We use confocal microscopy to detect closet bacteria near the epithelium. 16S sequence was used for the composition of microbiota. Compared with control mice, those receiving emulsifier, showed impaired glycemic tolerance, hyperinsulinemia, altered liver enzymes, larger mitochondria and increased gall bladder size. Additionally, mice in the experimental group showed higher levels of DCA, reduced Muc2 RNA expression, reduced m...

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Section: S Rrna Gene Sequence Analysismentioning

confidence: 99%

Food Additive P-80 Impacts Mouse Gut Microbiota Promoting Intestinal Inflammation, Obesity and Liver Dysfunction

Singh¹

2016

SOJMID

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“…Additionally, also antibiotic treatment of obese mice was described as an important dysbiotic effect affecting Firmicutes/Bacteroidetes vs. Proteobacteria ratio (11). Besides hyperglycemia (12), insulin resistance was associated with gut microbiota changes, even in patients with similar body weight, strongly correlating with obesity and type 2 diabetes mellitus (13). The mechanisms can be included in the cross-talk between gut microbiota-inflammasome machinery.…”

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confidence: 99%

The human gut microbiota: a dynamic interplay with the host from birth to senescence settled during childhood

et al. 2014

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“…Diğer yandan intestinal mikrobiyota, immün sistem dışında birçok metabolik olayda etkili olmaktadır. Günümüzde gastrointestinal sistem mikrobiyotasının obezite, diyabetes mellitus, ateroskleroz ve alkolik olmayan yağlı karaciğer hastalığı patogenezinde etkili olduğu bilinmektedir [15][16] .…”

Section: Gastrointestinal Mikrobiyomunclassified

Normal Mikrobiyal Floranın İnsan Sağlığı Üzerine Etkisi ve İnsan Mikrobiyom Projesi

Bozok¹,

Şimşek²,

Kömür³

et al. 2014

Arşiv Kaynak Tarama Dergisi

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ÖZETVücut alanımızı paylaştığımız mikroorganizmaların varlığı eskiden beri bilinen bir gerçektir. Ancak yaşam şeklimizdeki değişiklikler ve özellikle de karşılaştığımız yeni moleküler bileşikler, bu mikroorganizmalar üzerinde çeşitli etkilere neden olmaktadır. Son zamanlarda geliştirilen yeni nesil sekanslama teknikleri, bu mikroorganizma topluluklarındaki değişikliklerin oldukça ayrıntılı bir biçimde analiz edilmesine imkan vermektedir. Yapılan son çalışmalar da bize bu mikroorganizmaların topluluklarının insan sağlığı üzerinde tahmin edilenden daha fazla etkiye sahip olduğunu göstermektedir. Anahtar kelimeler: Mikrobiyom, sekanslama, moleküler bileşik ABSTRACT Presence of microorganisms that we share our body has been an already known fact. However, changes in our way of life and particularly new molecular compounds we encounter, cause several effects on microorganisms. Recently developed new generation sequencing techniques, allow detailed analysis of the changes in these microbial communities. Recent studies indicate that, these communities of microorganisms have more impact than predicted on human health.

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Molecular Characterisation of the Faecal Microbiota in Patients with Type II Diabetes

Cited by 408 publications

References 41 publications

Food Additive P-80 Impacts Mouse Gut Microbiota Promoting Intestinal Inflammation, Obesity and Liver Dysfunction

Food Additive P-80 Impacts Mouse Gut Microbiota Promoting Intestinal Inflammation, Obesity and Liver Dysfunction

The human gut microbiota: a dynamic interplay with the host from birth to senescence settled during childhood

Normal Mikrobiyal Floranın İnsan Sağlığı Üzerine Etkisi ve İnsan Mikrobiyom Projesi

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