Molecular Characterisation of the Species of the Genus Zygosaccharomyces

Microbial quality and yeast population dynamics in cracked green table olives’ fermentations

“…The genus Zygosaccharomyces to which Z. mrakii previously belonged to is known for including the major spoiler microorganisms in fruit juices, sauces, soft drinks, alcoholic beverages among others. In fact, the presence of species of that genus is normally an indicator of future spoilage despite their capacity of eventually confer to fermented food useful organoleptic qualities (Esteve-Zarzoso et al, 2003;Loureiro & Malfeito-Ferreira, 2003).…”

“…Fragment sizes were estimated by comparison against a DNA ladder (100 bp BioRad). Yeasts were identified to the species level by comparison of the amplified products and their restrictionefragments profiles with those described by Coton, Coton, Levert, Casaregola, & Sohier, 2006;Esteve-Zarzoso et al, 1999;Esteve-Zarzoso, Zorman, Belloch, & Querol, 2003;Guillamón, Sabaté, Barrio, Cano, &Querol, 1998 andde Llanos Frutos, Fernandez-Espinar, &Querol, 2004. A representative number of isolates corresponding of each PCR-RFLP profile was selected and sequence analysis of the domains D1 and D2 of the 26S rDNA region was accomplished.…”

A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar

“…PCR and restriction analysis of the 5.8S-ITS region of Zygosaccharomyces type strains was also considered a rapid and effective method for differentiating yeast species involved in food and beverage fermentation and spoilage. This method has been able to assign different restriction fragment length polymorphism (RFLP) fingerprinting to every species, except for Z. cidri and Z. fermentati (Esteve-Zarzoso et al, 2003). However, intraspecific variation in the ITS region had already been reported by some authors within the species Z. rouxii and Z. bailii (Kurtzman and Robnett, 2003;James et al, 1996).…”

Genome size and ploidy level: New insights for elucidating relationships in Zygosaccharomyces species

“…Even though heterogeneity at intraspecific level has also been observed, generally the patterns of strains belonging to the same species are very similar. Thus, the PFGE technique has been proposed as a powerful tool for differentiating Zygosaccharomyces species (Esteve-Zarzoso et al, 2003). However, difficulties related to band size estimation, co-migrating chromosomes, and electrophoretic conditions, can complicate the interpretation of PFGE results, mainly when arisen from different laboratories (Keogh et al, 1998).…”

“…Z. rouxii ABT 808 and Z. mellis ABT 401strains were used for comparison in PFGE experiment. The chromosomal bands were comparable in size and number to those of yeasts belonging to the genus Zygosaccharomyces, which are in low number ranging from 3 to 8 bands and whose electrophoretic separation requires long pulse times over a period of 5 days (De Jonge et al, 1986;Török et al, 1993;Oda and Tonomura, 1995;Sychrova et al, 2000;Esteve-Zarzoso et al, 2003). Two different migration times (100 and 120 h) were tested to resolve the most similar and large chromosomes ( Fig.…”