Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF

Pierzchała, Dorota; Liput, Kamila P.; Korwin-Kossakowska, A.; Ogłuszka, Magdalena; Poławska, Ewa; Nawrocka, Agata; Urbański, Paweł; Ciepłoch, Aleksandra; Juszczuk-Kubiak, Edyta; Lepczyński, Adam; Ślaska, Brygida; Kowal, Krzysztof; Pas, M.F.W. te; Śmiech, Magdalena; Leszczyński, Paweł; Taniguchi, Hiroaki; Fraser, Leyland; Sobiech, Przemysław; Sachajko, Mateusz; Herudzińska, Magdalena; Pareek, Chandra Shekhar; Pierzchała, Mariusz

doi:10.3390/ijms22136720

Cited by 9 publications

(7 citation statements)

References 113 publications

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“…The most classically used has been the two-dimensional (2D) gel electrophoresis followed by mass spectrometry (MS) or MALDI TOF-TOF analysis to identify protein spots. The 2D-MS/MALDI TOF-TOF has been used to identify 70 to 500 proteins in the peri-implantation period (d 9 to 16) and 820 proteins at mid-pregnancy (d 40 to 93) in the endometrium of pregnant and non-pregnant sows [ 11 – 13 , 20 , 21 ]. These proteomic studies have provided information on the dynamic physiological protein profile and how it may relate to maternal recognition and pregnancy.…”

Section: Discussionmentioning

confidence: 99%

In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

Gil,

Cambra,

Rodriguez-Martinez

et al. 2024

J Animal Sci Biotechnol

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Background Proteome characterization of the porcine endometrium and extraembryonic membranes is important to understand mother-embryo cross-communication. In this study, the proteome of the endometrium and chorioallantoic membrane was characterized in pregnant sows (PS) during early gestation (d 18 and 24 of gestation) and in the endometrium of non-pregnant sows (NPS) during the same days using LC-MS/MS analysis. The UniProtKB database and ClueGO were used to obtain functional Gene Ontology annotations and biological and functional networks, respectively. Results Our analysis yielded 3,254 and 3,457 proteins identified in the endometrium of PS and NPS, respectively; of these, 1,753 being common while 1,501 and 1,704 were exclusive to PS and NPS, respectively. In addition, we identified 3,968 proteins in the extraembryonic membranes of PS. Further analyses of function revealed some proteins had relevance for the immune system process and biological adhesion in endometrium while the embryonic chorion displayed abundance of proteins related to cell adhesion and cytoskeletal organization, suggesting they dominated the moment of endometrial remodeling, implantation and adhesion of the lining epithelia. Data are available via ProteomeXchange with identifier PXD042565. Conclusion This is the first in-depth proteomic characterization of the endometrium and extraembryonic membranes during weeks 3 to 4 of gestation; data that contribute to the molecular understanding of the dynamic environment during this critical period, associated with the majority of pregnancy losses.

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Section: Discussionmentioning

confidence: 99%

In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

Gil,

Cambra,

Rodriguez-Martinez

et al. 2024

J Animal Sci Biotechnol

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“…Up (Pregnant vs. non-pregnant) [46] Up (day 12 vs. day 9 of pregnant) [45] Up (9D vs. 16D of pregnancy) [47] Annexin A4 ANXA4 promotes trophoblast cell proliferation and invasion.…”

Section: Legumainmentioning

confidence: 99%

“…The pigs also serve as a valuable model to study pregnancy-related protein expression using proteomic approaches and have gained attention in the fields of biomedical and agricultural sciences [ 139 ]. Thus, proteomic approaches provide a better understanding and characterization of proteins involved in molecular and biological processes in pigs [ 47 ]. There are very few studies that describe the proteome profile of the early phase of pregnancy in pigs ( Table 1 ).…”

Section: Proteomics Of Pigs Uterine Milieu To Understand the Mechanis...mentioning

confidence: 99%

Proteomic Approaches to Unravel the Molecular Dynamics of Early Pregnancy in Farm Animals: An In-Depth Review

Jamwal,

Jena,

Tyagi

et al. 2023

JDB

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Infertility is a major problem in farm animals, which has a negative economic effect on farm industries. Infertility can be defined as the inability of animals to achieve a successful pregnancy. Early pregnancy is crucial to establish a successful pregnancy, and it is reported that 70–80% and 20–30% of total embryonic loss occur in cattle and pigs, respectively, during the first month of pregnancy. The advanced high-throughput proteomics techniques provide valuable tools for in-depth understanding of the implantation process in farm animals. In the present review, our goal was to compile, assess, and integrate the latest proteomic research on farm animals, specifically focused on female reproduction, which involves endometrial tissues, uterine fluids, oviductal fluids, and microRNAs. The series of studies has provided in-depth insights into the events of the implantation process by unfolding the molecular landscape of the uterine tract. The discussed data are related to pregnant vs. non-pregnant animals, pregnancy vs. oestrous cycle, different days of the early pregnancy phase, and animals with uterine infections affecting reproduction health. Some of the studies have utilized non-invasive methods and in vitro models to decipher the molecular events of embryo-maternal interaction. The proteomics data are valuable sources for discovering biomarkers for infertility in ruminants and new regulatory pathways governing embryo-uterine interaction, endometrium receptivity, and embryonic development. Here, we envisage that the identified protein signatures can serve as potential therapeutic targets and biomarkers to develop new therapeutics against pregnancy diseases.

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“…Recent studies have shown molecular changes in the porcine endometrium during the peri-implantation period and revealed sixteen differentially expressed proteins involved in the recognition and establishment of early gestation in pigs [ 21 ]. It has been hypothesized that CHEM may affect the proteome of the porcine uterus during implantation, and consequently influence the production of many factors essential for the proper course of gestation.…”

Section: Introductionmentioning

confidence: 99%

Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis

Bors

Dobrzyń

Kieżun

et al. 2022

Cells

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Chemerin (CHEM) is a hormone mainly expressed in adipocytes involved in the regulation of energy homeostasis and inflammatory response. CHEM expression has been demonstrated in the structures of the porcine hypothalamic-pituitary-gonadal axis, as well as in the uterus, trophoblasts and conceptuses of pigs. In this study, we performed high-throughput proteomic analyses (liquid chromatography with tandem mass spectrometry, LC-MS/MS) to examine the influence of CHEM (400 ng/mL) on differentially regulated proteins (DRPs) in the porcine endometrial tissue explants during implantation (15 to 16 days of gestation). Among all 352 DRPs, 164 were up-regulated and 188 were down-regulated in CHEM-treated group. DRPs were assigned to 47 gene ontology (GO) terms (p-adjusted < 0.05). Validation of four DRPs (IFIT5, TGFβ1, ACO1 and PGRMC1) by Western blot analysis confirmed the veracity and accuracy of the LC-MS/MS method used in the present study. We suggest that CHEM, by modulating various protein expressions, takes part in the endometrial cell proliferation, migration and invasion at the time of implantation. It also regulates the endometrial immune response, sensitivity to P4 and the formation of new blood vessels. Additionally, CHEM appears to be an important factor involved in endothelial cell dysfunction during the pathogenesis of preeclampsia. The identification of a large number of DRPs under the influence of CHEM provides a valuable resource for understanding the molecular mechanisms of this hormone action during implantation, which is a prerequisite for better control of pig reproduction.

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Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF

Cited by 9 publications

References 113 publications

In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

In-depth proteome characterization of endometrium and extraembryonic membranes during implantation in pig

Proteomic Approaches to Unravel the Molecular Dynamics of Early Pregnancy in Farm Animals: An In-Depth Review

Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis

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