Molecular characteristics and virulence gene profiles of Staphylococcus aureus causing bloodstream infection

Li, Xuehan; Fang, Fang; Jin, Zhao; Lou, Ning; Li, Chenglin; Huang, Tao; Li, Yirong

doi:10.1016/j.bjid.2018.12.001

Cited by 45 publications

(79 citation statements)

References 35 publications

(49 reference statements)

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“…Chromosomal DNAs were extracted from S. aureus isolates as described previously [23]. The extracted chromosomal DNAs were stored at -20°C for spa, Staphylococcus chromosomal cassette mec (SCCmec), and multilocus sequence typing, and detection of virulence genes.…”

Section: Staphylococcal Protein a (Spa) Typingmentioning

confidence: 99%

“…The extracted chromosomal DNAs were stored at -20°C for spa, Staphylococcus chromosomal cassette mec (SCCmec), and multilocus sequence typing, and detection of virulence genes. For spa typing, the variable repeat region of spa was amplified using oligonucleotide primers [23,24] (see Table 1) followed by sequencing. The PCR mixture and conditions were similar to those described previously [23].…”

Section: Staphylococcal Protein a (Spa) Typingmentioning

confidence: 99%

“…For spa typing, the variable repeat region of spa was amplified using oligonucleotide primers [23,24] (see Table 1) followed by sequencing. The PCR mixture and conditions were similar to those described previously [23]. The resulting amplicons were purified and subjected to Sanger dideoxy DNA sequencing (Tianyihuiyuan, China) followed by analysis using the Ridom web server (http://spaserver.ridom.de).…”

Section: Staphylococcal Protein a (Spa) Typingmentioning

confidence: 99%

“…MLST was carried out according to the protocol described previously [23,25]. Seven housekeeping genes of S. aureus, namely arcC, aroE, glpF, gmk, pta, tpi, and yqil, were adopted for MLST.…”

Section: Multilocus Sequence Typing (Mlst)mentioning

confidence: 99%

“…Eleven virulence genes, including the Panton-Valentine leukocidin (pvl), the staphylococcal enterotoxin genes (sea, seb, sec), the exfoliative toxin genes (eta, etb), the hemolysin gene (hla, hlb), and the adhesion factor genes (fnbA, fnbB, clfA) were detected using PCR assays. The PCR mixture and conditions were similar to those described previously [23]. The common ERY resistance genes (ermA, ermB, ermC), and the TET resistance genes (tetL, tetK, tetM, tetO) were examined using PCR assays as previously described [27,28].…”

Section: Detection Of Virulence Genes and Antibiotic Resistance Genesmentioning

confidence: 99%

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Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

Huang

et al. 2019

Preprint

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Background: There have been no reports regarding the molecular characteristics, virulence features, and antibiotic resistance profiles of Staphylococcus aureus ( S. aureus ) from Hainan, the southernmost province of China. Methods: 227 S. aureus isolates, consisting of 76 methicillin-resistant S. aureus (MRSA) and 151 methicillin-susceptible S. aureus (MSSA), were collected in 2013-2014 and 2018-2019 in Hainan, and investigated for their molecular characteristics, virulence genes, antibiotic resistance profiles and main antibiotic resistance genes. Results: Forty sequence types (STs) including three new STs (ST5489, ST5492 and ST5493), and 79 Staphylococcal protein A ( spa ) types were identified based on multilocus sequence typing (MLST) and spa typing, respectively. ST398 (14.1%, 32/227) was found to be the most prevalent, and the prevalence of ST398-MSSA increased significantly from 2013-2014 (5.5%, 5/91) to 2018-2019 (18.4%, 25/136). Seventy-six MRSA isolates were subject to staphylococcus chromosomal cassette mec (SCC mec ) typing. SCC mec- IVa was the predominant SCC mec type, and specifically, ST45-SCC mec IVa, an infrequent type in mainland China, was predominant in S. aureus from Hainan. The antibiotic resistance profiles and antibiotic resistance genes of S . aureus show distinctive features in Hainan. The resistant rates of the MRSA isolates to a variety of antibiotics were significantly higher than those of the MSSA isolates. The predominant erythromycin and tetracycline resistance genes were ermC (90.1%, 100/111) and tetK (91.8%, 78/85), respectively. Eleven virulence genes, including the Panton-Valentine leukocidin ( pvl ) and eta , were determined, and the frequency of eta and pvl were found to be 57.3% and 47.6%. Such high prevalence has never been seen in mainland China before. Conclusion: S. aureus isolates in Hainan have unique molecular characteristics, virulence gene and antibiotic resistance profiles, and main antibiotic resistance genes which may be associated with the special geographical location of Hainan and local trends in antibiotic use.

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Section: Staphylococcal Protein a (Spa) Typingmentioning

confidence: 99%

Section: Staphylococcal Protein a (Spa) Typingmentioning

confidence: 99%

Section: Staphylococcal Protein a (Spa) Typingmentioning

confidence: 99%

Section: Multilocus Sequence Typing (Mlst)mentioning

confidence: 99%

Section: Detection Of Virulence Genes and Antibiotic Resistance Genesmentioning

confidence: 99%

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Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

Huang

et al. 2019

Preprint

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Prevalence and characterization of Staphylococcus aureus from different processing points in beef processing plants in Shandong, China

Zhang,

Liu,

Yang

et al. 2024

Food Safety and Health

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Staphylococcus aureus (S. aureus) is a common zoonotic foodborne pathogen that poses a serious threat to the microbial control of meat processing, especially the multidrug resistant (MDR) strain. This study aimed to investigate the prevalence, antimicrobial susceptibility, virulence genes, biofilm formation ability (BFA), and molecular types of S. aureus isolated from two beef‐processing plants in China. The results revealed that the prevalence of S. aureus was 17.1% (60/350), including one methicillin‐resistant strain. The chilled carcass showed the highest detection rate among the seven sampling points. The isolates exhibited high resistance to penicillin (88.3%) and lincomycin (80.0%), and 75.0% of the isolates showed multidrug resistance (MDR). All isolates carried hla and clfa and critical toxin genes (seb, pvl, and tst) were also detected. Additionally, 96.7% of isolates exhibited strong and moderate BFA. The isolates were typed by Multilocus Sequence Typing (MLST) and Staphylococcus Protein A (spa) typing methods, resulting in nine different types for each method. The predominant type was ST7‐t091, accounting for 68.3% of the isolates. Our research suggests that there may be a critical control point in the chilling room, and the high MDR rate and strong BFA increase the difficulty of control and the risk of transmission.

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Methicillin-resistant Staphylococcus aureus of the clonal lineage ST5-SCCmecII-t2460 was associated with high mortality in a Wuhan hospital

Zhang

et al. 2021

Braz J Microbiol

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Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen that can cause serious infectious diseases. An emerging MRSA strain, ST5-SCCmecII spa-type-t2460 (SMRSA), has spread rapidly since its recent emergence in China, but little information is available about this lineage. In this study, 91 MRSA isolates were collected from patients treated in the Zhongnan Hospital, Wuhan University, from 2018 to 2019, and investigated for their molecular characteristics, antibiotic resistance profiles, and clinical characteristics. The predominant lineage, SMRSA, accounted for 37.4% (34/91) of the isolates, followed by ST239-SCCmecIII-t030 (19.8%, 18/91) and ST59-SCCmecIV-t437 (8.8%, 8/91). In contrast to the latter two non-SMRSA (nSMRSA) lineages, which are among the main MRSA found in Chinese settings, SMRSA exhibited small colony variant (SCV) phenotype and had extremely high resistance rates to erythromycin (100.0%), clindamycin (100.0%), levofloxacin (100.0%), tetracycline (97.1%), moxifloxacin (97.1%), and ciprofloxacin (100%), but was more susceptible to rifampicin (resistance rate 3%). The levels of white blood cells (WBC) and procalcitonin (PCT) and the 30-day mortality in patients infected with SMRSA were (12.54 ± 6.61) × 109/L, 0.66 ng/mL, and 52.9%, respectively, which were much higher than those in patients infected with nSMRSA. In addition, patients infected with SMRSA were more frequently admitted to the intensive care unit (ICU) and submitted to invasive procedures than those infected with nSMRSA. In conclusion, SMRSA showed SCV phenotype and exhibited multiple antibiotic-resistance profiles. In this study, SMRSA was associated with serious infections and poor prognosis. Compared with ST239, ST59, or other nSMRSA strains, patients infected with SMRSA strains have higher 30-day mortality, increased levels of inflammatory biomarkers, and more frequent ICU hospitalization and invasive procedures.

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Molecular characteristics and virulence gene profiles of Staphylococcus aureus causing bloodstream infection

Cited by 45 publications

References 35 publications

Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

Prevalence and characterization of Staphylococcus aureus from different processing points in beef processing plants in Shandong, China

Methicillin-resistant Staphylococcus aureus of the clonal lineage ST5-SCCmecII-t2460 was associated with high mortality in a Wuhan hospital

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