Molecular characteristics isolates of rabies virus isolated from humans in Ukraine

Polupan, I. М.; Недосеков, В. В.; Степанова, Татьяна Ивановна; Rudoi, O.V.; Parshikova, A.V.; Drozdova, E. I.

doi:10.1088/1755-1315/677/4/042025

Cited by 2 publications

(4 citation statements)

References 10 publications

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“…Quoting Baker et al (2020), incursions from other regions account for less than 1.0% of cases but allow for re-emergence of the disease. The aforementioned also was submitted by Polupan et al (2021) during their study, the genetic sequences of the studied samples from Ukraine are characteristic of isolates isolated in the Russian Federation, South-Eastern Europe, Western Siberia, and Kazakhstan. Similar results were received by Picard-Meyer et al (2012) when they investigated samples from Ukraine.…”

Section: Discussionmentioning

confidence: 99%

“…During the last few decades, more than 100,000 people (almost 210 per 100,000 population) turned to medical institutions for treatment of animal bites annually. Every year, up to 23,000 people have been prescribed rabies vaccinations (Kornienko et al, 2019;Polupan et al, 2021). Historically, before 1970, dogs were a common reservoir and sources of rabies in Ukraine, however, due to the increasing population of wildlife, the main reservoir and source of rabies in Ukraine became red foxes (Vulpes vulpes) (Botvinkin & Kosenko, 2004;Baker et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, during 1997-2017, cats were the source of rabies for humans in 39.6% of cases, dogs -in 29.3% and foxes -only in 20.7%, others -8.7%, bats -1.7%. The most meaningful factors of the increasing role of domestic carnivores as a source of rabies for humans are low levels of specific rabies prevention in domestic animals and the increased density of the population of stray dogs and cats (Polupan et al, 2021). Among the most important preventive measures which need to covered by monitorring are problems of irresponsible pet ownership, stray dogs and cats, the possibility of full immune prophylaxis of rabies (both in humans and animals).…”

Section: Introductionmentioning

confidence: 99%

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Current issues and gaps in the implementation of rabies prevention in Ukraine in recent decades

Makovska

Krupinina

Недосеков

et al. 2021

Regul. Mech. Biosyst.

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Ukraine remains the only country in Europe where rabies is widespread among animals and humans. Annually there are about 1,600 rabies cases in animals in Ukraine and sporadic cases in humans have been registered despite the conducting of preventive measures. Therefore, the aim of the study was to inspect the failures in rabies prevention, indicate the top reasons for human cases and highlights the risk of animal attacks in view of their species and geographical distribution in Ukraine during 1996–2020. The following archival state materials were used for analysis: from the Ministry of Health of Ukraine and from the annual reports of oblast departments of the State Service of Ukraine for Food Safety and Consumer Protection. In general, more than 84,000 people (187.4 per 100,000 of the population) were affected by bites or harmful contact with animals every year, among them, 2,155 people were victims of rabid animals. Post-exposure prophylaxis (PEP) was prescribed annually, on average, for 21,434 patients (25.5% of all victims). Most people were attacked by cats and dogs that had owners (71.5%). The frequency of the proportion of the risk of attacks by rabid dogs on humans was (1:124), from cats (1:25), wild animals (1:7), and farm animals (1:2), but the largest general proportion of animal attacks on people was from dogs – 838,635 attacks (77.7%). Thus, due to the permanently higher level of contact with people, attacks by dogs remains more dangerous. Geographically attacks on humans by domestic carnivores were observed most commonly in the east part of Ukraine due to the high urbanization of the region and the high density of the human population. A large number of attacks by foxes was observed in the west part of Ukraine due to the larger area of forests and fields. During the last 25 years, there have been 63 human rabies cases. The main sources of rabies were dogs (24 cases) and cats (22 cases). The main causes of development of rabies were: failure to receive the PEP due to the absence of a visit to a hospital after an attack of an animal (n = 38), failures in prescribing PEP (n = 15), failure of PEP (n = 10). In conclusion, the gaps in the control measures against rabies are the lack of agreed coordination of inter-sectoral links, the lack of significant efforts to raise public awareness and the lack of funding for prophylaxis programmes for humans and animals. Our future research will be aimed at modelling the transmission of rabies from the pet population to humans.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Current issues and gaps in the implementation of rabies prevention in Ukraine in recent decades

Makovska

Krupinina

Недосеков

et al. 2021

Regul. Mech. Biosyst.

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“…У комплексі всіх антирабічних заходів, що застосовуються для контролю цього захворювання, поряд із використанням цілого арсеналу вакцинних засобів (парентеральна та пероральна вакцинація тварин), загальних протиепізоотичних засобів (регулювання популяції диких тварин, гуманне регулювання чисельності безпритульних тварин), важливою ланкою є епізоотичний нагляд, який є базовою складовою будь-якої програми контролю сказу. Однак, достовірність епізоотичного нагляду базується виключно на результатах лабораторних досліджень, адже заключний діагноз «сказ» можна поставити тільки на підставі постмортальних результатів лабораторних досліджень головного мозку [3,4]. Сказ є основним серед зоонозів, для якого діагностичні методи були стандартизовані на міжнародному рівні [5,6].…”

Section: реакція прямої імунофлуоресценції в лабораторній діагностиці...unclassified

Direct fluorescent antibody test in laboratory diagnosis of animal rabies in Ukraine

Polupan¹

2021

Veterinary Medicine: Inter-Departmental Subject Scientific Collection

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The aim of the study was to analyze the role of the direct fluorescent antibody (DFA) test in the system of laboratory diagnosis of animal rabies in Ukraine. For the analysis, materials of official veterinary reporting were used according to Form No. 2-VET “Report on the work of the state laboratories of veterinary medicine” regarding the results of laboratory studies of pathological material suspicious of rabies, the State Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Expertise (SRILDVSE) and virology departments of the State Regional Laboratories of the State Food and Consumer Service of Ukraine, and analytical materials: Report “On the assessment of the risk of spread of rabies among domestic and farm animals under the current animal rabies control system in Ukraine” and the Report “On the assessment of the risk of spread of rabies among wild animals in Ukraine”. It has been determined that, over the past 15 years (2006–2020), 194,079 tests of the pathological material were carried out in state laboratories. The direct fluorescent antibody test is the main technique for the diagnosis of animal rabies in Ukraine, when used in 94.5% of cases, the final diagnosis of rabies is made. We have used standardization of approaches, including the use of the reference rabies virus CVS-11 (ATCC VR 959), to the organization and conducting of interlaboratory rounds of professional testing VET-TEST to identify of rabies virus antigen within the requirements ISO 17043:2017 “Conformity assessment. General requirements for testing professional level”. DFA test is the main reaction for the diagnosis of rabies in animals in Ukraine. Standardized approaches were introduced and interlaboratory rounds of professional testing BET-TEST have been organized in 2020 for the detection of rabies virus antigen in accordance with the quality standard ISO 17043:2017. The necessity of introducing new methods of laboratory diagnostics of rabies, such as viral isolations in tissue culture and polymerase chain reaction, has been established

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Molecular characteristics isolates of rabies virus isolated from humans in Ukraine

Cited by 2 publications

References 10 publications

Current issues and gaps in the implementation of rabies prevention in Ukraine in recent decades

Current issues and gaps in the implementation of rabies prevention in Ukraine in recent decades

Direct fluorescent antibody test in laboratory diagnosis of animal rabies in Ukraine

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