Molecular Characteristics of Antimicrobial Resistance and Virulence in Klebsiella pneumoniae Strains Isolated from Goose Farms in Hainan, China

Bai, Shuan-Cheng; Yu, Yang; Kuang, Xu; Li, Xianan; Wang, Min-Ge; Sun, Ruan-Yang; Sun, Jian; Liu, Ya-Hong; Liao, Xiao‐Ping

doi:10.1128/aem.02457-21

Cited by 8 publications

(9 citation statements)

References 41 publications

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“…It is of note that metal tolerance operons ( pco plus sil ) were mainly located in multireplicon F type (FII K plus FIB K ) plasmids carrying genes encoding resistance to several classes of antibiotics, including the critical extended-spectrum cephalosporins and/or fluoroquinolones, supporting the potential for diverse coselection events ( 67 , 68 ). These mosaic F type plasmids are common in K. pneumoniae populations from different sources ( 68 – 73 ), suggesting a major role in both dissemination and persistence of antibiotic resistance and metal tolerance genes and K. pneumoniae adaptation to different niches. Besides, the similarity between plasmid backbones identified in poultry isolates and those described in K. pneumoniae collections from humans (see Tables S3 and S4 in the supplemental material) ( 72 ) suggests a common pool of shared plasmids between humans and eventually different animal species ( 70 ), which deserves to be further explored with comprehensive comparative plasmidome analysis.…”

Section: Discussionmentioning

confidence: 99%

From Farm to Fork: Persistence of Clinically Relevant Multidrug-Resistant and Copper-Tolerant Klebsiella pneumoniae Long after Colistin Withdrawal in Poultry Production

et al. 2023

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Section: Discussionmentioning

confidence: 99%

From Farm to Fork: Persistence of Clinically Relevant Multidrug-Resistant and Copper-Tolerant Klebsiella pneumoniae Long after Colistin Withdrawal in Poultry Production

et al. 2023

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“…It is of remark that metal tolerance operons (pco+sil) were mainly located in multireplicon F-type (FIIK+FIBK) plasmids carrying genes encoding resistance to several classes of antibiotics, including the critical extended-spectrum cephalosporins and/or fluoroquinolones, supporting the potential for diverse co-selection events (67,68). These mosaic F-type plasmids are common in K. pneumoniae populations from different sources (68)(69)(70)(71)(72)(73), suggesting a major role in both dissemination and persistence of antibiotic resistance and metal tolerance genes, and K. pneumoniae adaptation to different niches. Besides, the similarity between plasmid backbones .…”

Section: Discussionmentioning

confidence: 99%

From farm to fork: persistence of clinically-relevant multidrug-resistant and copper-tolerantKlebsiella pneumoniaelong after colistin withdrawal in poultry production

Mourão

Ribeiro-Almeida

Novais

et al. 2023

Preprint

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The concern of colistin-resistant bacteria in animal-food-environmental-human ecosystems prompted the poultry sector to implement colistin restrictions and explore alternative trace metals/copper feed supplementation. The impact of these strategies on the selection and persistence of colistin-resistant Klebsiella pneumoniae (Kp) in the whole poultry-production chain needs clarification. We assessed colistin-resistant and copper-tolerant Kp occurrence in chicken raised with inorganic and organic copper-formulas from one-day-old chicks to meat (7 farms/2019-2020), after long-term colistin withdrawal (>2-years). Clonal diversity and Kp adaptive features were characterized by cultural, molecular, and whole-genome-sequencing (WGS) approaches. Most chicken-flocks (75%) carried Kp at early+pre-slaughter stages, with a significant decrease (p<0.05) in meat batches (17%) and sporadic water/feed contamination. High rates (>50%) of colistin-resistant/mcr-negative Kp were observed among faecal samples, independently of feed. Most samples carried multidrug-resistant (90%) and copper-tolerant isolates (81%; pco+sil/MICCuSO4 ≥16mM). WGS revealed accumulation of colistin resistance associated mutations and F-type multireplicon plasmids carrying antibiotic resistance and metal/copper-tolerance genes. The Kp population was polyclonal, with various lineages dispersed throughout poultry production. ST15-KL19, ST15-KL146 and ST392-KL27, and IncF plasmids were similar to those from global human clinical isolates, suggesting chicken-production as a reservoir/source of clinically-relevant Kp lineages and genes with potential risk to humans through food and/or environmental exposure. Despite long-term colistin ban limited mcr spread, it was ineffective in controlling colistin-resistant/mcr-negative Kp, regardless of feed. This study provides crucial insights into the persistence of clinically-relevant Kp in the poultry-production chain and highlights the need for continued surveillance and proactive food safety actions within a One-Health perspective.

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“…In goosefsh integrated farms, the increase in water geese stocking density has also led to bacterial pollution. Excessive use of antimicrobial agents in feed has been reported to reduce bacterial infections in geese and has a negative efect on antimicrobial resistance [41,42]. Tese data imply that antimicrobial resistance may spread from livestock to the aquatic environment.…”

Section: Discussionmentioning

confidence: 99%

High Occurrence of Multiple-Drug Resistance Mediated by Integron in Aeromonas Isolated from Fish-Livestock Integrated Farms

Deng

Feng

Tan

et al. 2023

Aquaculture Research

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Fish-livestock integrated farming is a traditional practice in South China and Southeast Asia. The administration of antimicrobial agents in livestock may potentially facilitate the spread of antimicrobial resistance to aquaculture through the drainage of livestock manure. Aeromonas spp. are fish pathogens that are the predominant bacteria in water bodies. The aim of this study was to characterize multiple-drug-resistantAeromonas spp. isolated from integrated and nonintegrated fish-livestock farms and to investigate the occurrence of class 1 integrons. Of the 481 Aeromonas strains examined in this study, isolates from pig manure, fish, and environmental samples from pig-fish or goose-fish integrated farms were significantly more resistant to various antimicrobial agents than those from nonintegrated farms P < 0.05 . High rates of resistance to nalidixic acid and sulfamonomethoxine were observed in the isolates from the integrated farm. Fifty Aeromonas isolates (10.4%) from integrated farms contained class 1 integrons. In addition, 96.0% (48/50) of the integron-positive strains displayed multiple-drug resistance. Ten types of gene cassette arrays were determined by sequencing, including dfrA17, dfrA12-orfF-aadA2, catB8, dfrB4-catB3-aadA1, aac6(6′)-Ib-cr-arr-3, aac-II-blaOXA-21-catB3, aar2-aacA4-drfA1-orfC, aac(6′)-Ib-cr, dfrA15, and dfrB4-catB3-blaOXA-10-aadA1. Notably, among the 50 integron-positive isolates, twenty isolates showed integrons located in plasmids, which may facilitate the transmission of resistance in integrated farms. Our investigation confirmed the high prevalence of multiple antimicrobial resistances mediated by class 1 integrons in Aeromonas isolates from integrated farms. Therefore, it is necessary to establish a risk assessment method for antimicrobial resistance in aquaculture.

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Molecular Characteristics of Antimicrobial Resistance and Virulence in Klebsiella pneumoniae Strains Isolated from Goose Farms in Hainan, China

Cited by 8 publications

References 41 publications

From Farm to Fork: Persistence of Clinically Relevant Multidrug-Resistant and Copper-Tolerant Klebsiella pneumoniae Long after Colistin Withdrawal in Poultry Production

From Farm to Fork: Persistence of Clinically Relevant Multidrug-Resistant and Copper-Tolerant Klebsiella pneumoniae Long after Colistin Withdrawal in Poultry Production

From farm to fork: persistence of clinically-relevant multidrug-resistant and copper-tolerantKlebsiella pneumoniaelong after colistin withdrawal in poultry production

High Occurrence of Multiple-Drug Resistance Mediated by Integron in Aeromonas Isolated from Fish-Livestock Integrated Farms

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