Molecular characteristics of community-acquired, methicillinresistant<i>Staphylococcus aureus</i>isolated from Chinese children

Geng, Wenjing; Yang, Yonghong; Wu, Dejing; Huang, Guoying; Wang, Chuanqing; Deng, Li; Zheng, Yuejie; Fu, Zhou; Li, Changcong; Shang, Yunxiao; Zhao, Chuanqi; Yu, Sangjie; Shen, Xuzhuang

doi:10.1111/j.1574-695x.2009.00648.x

Cited by 57 publications

(52 citation statements)

References 32 publications

(34 reference statements)

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“…These isolates belong to the dominant Taiwan C-MRSA clones (CC59:SCCmec V:pvl-positive and spa CC t437). Since CC59:SCCmec V isolates have now been reported in other countries (7,10,13,15,19) and since other C-MRSA lineages have also been found to have low-level ␤-lactam resistance (23,28,30), the findings and implications of our study may be relevant to predominant C-MRSA lineages on other continents. Our results indicate that as more clinical laboratories switch to using automated susceptibility testing instruments for determining antimicrobial susceptibility, there is a need for careful evaluation of the performance of these methods for the detection of mecA-positive S. aureus with a low-level oxacillin resistance to prevent their further spread in health care and community settings.…”

Section: Discussionsupporting

confidence: 54%

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mecA -Positive Staphylococcus aureus with Low-Level Oxacillin MIC in Taiwan

et al. 2012

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Although the presence of mecA is the genotypic determinant of methicillin-resistant Staphylococcus aureus (MRSA), certain MRSA strains, especially community-associated MRSA (C-MRSA), can display an oxacillin MIC in the Clinical and Laboratory Standards Institute susceptible breakpoint range (<2 g/ml). Among 91 and 180 isolates thought to be methicillin-susceptible S. aureus (MSSA) with oxacillin MICs of 2 and 1 g/ml as determined by the Sensititre broth microdilution test initially, 52 (57.1%) and 6 (3.3%), respectively, were mecA positive. These mecA-positive low-oxacillin-MIC isolates belong to the dominant Taiwan C-MRSA clone (clonal complex [CC] 59), 56 of which carried SCCmec type V and were pvl positive, and 43 of which belonged to spa CC t437. All 271 isolates were retested by Sensititre, as well as by Vitek II and disk diffusion (DD). Based on the oxacillin results, the sensitivities of the Sensititre, Vitek II, and DD methods were 48.3% (28/58), 46.6% (27/58), and 89.6% (52/ 58), respectively. Although cefoxitin was better at detecting these isolates, 12.1, 10.4, and 5.2% of these isolates were still misidentified as MSSA by Sensititre, Vitek II, and DD, respectively. These results highlight the difficulty in the accurate identification of MRSA with borderline oxacillin MICs in the CC59:SCCmec V clone, which likely has contributed to its spread in the health care and community settings. Since this clone has now been detected in other countries, and since other C-MRSA lineages have also been found to have low-level ␤-lactam resistance, the findings of the present study may be relevant to other regions. Further studies are warranted to determine the extent and clinical impact of such misidentification.

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Section: Discussionsupporting

confidence: 54%

“…This clone also accounted for 13.1% of C-MRSA isolated from Chinese children in multiple hospitals in China (10). In Europe, the ST59:SCCmec V MRSA has also been detected in the Netherlands (15), while SCCmec V carrying ST338, a single-locus variant of ST59, has also been found in Poland (19).…”

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confidence: 89%

mecA -Positive Staphylococcus aureus with Low-Level Oxacillin MIC in Taiwan

et al. 2012

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“…The ST6 MRSA strain has rarely been isolated, but community-associated MRSA (CA-MRSA) clones were reported in Kuwait hospitals (32), Japan (12), and Lebanon (31). According to previous research, ST6 is not the predominant S. aureus lineage that is observed in hospitals and animals in China (6,8,9,35,36,38,40,41). Why ST6 MSSA became the dominant ST that causes SFP and whether ST6 was the unique ST in food products or among SFP isolates in China should be investigated further.…”

Section: Discussionmentioning

confidence: 96%

Characterization of Staphylococcus aureus Strains Associated with Food Poisoning in Shenzhen, China

Yan

Wang

Tao

et al. 2012

Appl Environ Microbiol

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ABSTRACTTo characterize isolates ofStaphylococcus aureusthat were associated with staphylococcal food poisoning between 2006 and 2009 in Shenzhen, Southern China, a total of 52Staphylococcus aureusisolates from 11 outbreaks were analyzed by using multilocus sequence typing (MLST),spatyping, and pulsed-field gel electrophoresis (PFGE). PCR analysis was used to analyze the staphylococcal enterotoxin (SE) genesseatosei, and antimicrobial susceptibility testing was also performed. ST6 was the most dominant sequence type (ST), constituting 63.5% (34/52) of all of the isolates in 7 outbreaks. The next most common ST was ST943, which constituted 23.1% (12/52) of the isolates that were collected from 3 outbreaks. t701, t091, and t2360 were the most predominantspatypes, constituting 67.3% (35/52) of the isolates that were collected from 11 outbreaks. Three PFGE types, (types A, B, and C) were the most frequently observed types, constituting 84.6% (44/52) of all of the isolates. The enterotoxin gene that we detected most frequently wassea(45/52; 86.5%). Four SE gene profiles were observed, includingsea(n= 45),sec-seh(n= 3),seb(n= 2), andseg-sei(n= 2). With respect to antibiotic resistance, penicillin resistance was the most common (96.2%; 50/52), followed by resistance to tetracycline (28.8%; 15/52). Approximately 30.8% (16/52) of the isolates were resistant to at least two antibiotics, and 7.7% (4/52) of the isolates were resistant to three or more drugs. The two predominantS. aureuslineages, (i) PFGE types A and B with ST6 and (ii) PFGE type C with ST943, were identified in the outbreaks.

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“…Autoclaved agar medium (10 ml) was poured into sterile Petri dishes under sterile conditions. A lawn of meticillin-resistant S. aureus skin isolate ST59-MRSA-IV (Geng et al, 2010) was prepared on an ISP2 agar plate. Thereafter, a paper disc containing B. subtilis NCBI 3610 (undomesticated wild-type) was placed in the middle of the S. aureus ST59 lawn and allowed to grow overnight.…”

Section: Methodsmentioning

confidence: 99%

“…The molecular mechanisms by which these products work are poorly understood. Similarly, S. aureus, a pre-eminent human pathogen causing an array of serious hospital-or community-acquired infections worldwide (Geng et al, 2010;Klevens et al, 2007;Otto, 2010), is found on human skin, digestive tracts, nares, livestock and surgical instrumentation (Iwase et al, 2010;Otto, 2010;Roberson et al, 1994). At minimum, 30 % of the world population is colonized with S. aureus, a bona fide pathogen that has developed significant resistance against a variety of antibiotics and is the cause of more fatalities in the USA than HIV/AIDS (Enright et al, 2002;Klevens et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Microbial competition between Bacillus subtilis and Staphylococcus aureus monitored by imaging mass spectrometry

et al. 2011

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Microbial competition exists in the general environment, such as soil or aquatic habitats, upon or within unicellular or multicellular eukaryotic life forms. The molecular actions that govern microbial competition, leading to niche establishment and microbial monopolization, remain undetermined. The emerging technology of imaging mass spectrometry (IMS) enabled the observation that there is directionality in the metabolic output of the organism Bacillus subtilis when co-cultured with Staphylococcus aureus. The directionally released antibiotic alters S. aureus virulence factor production and colonization. Therefore, IMS provides insight into the largely hidden nature of competitive microbial encounters and niche establishment, and provides a paradigm for future antibiotic discovery.

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Molecular characteristics of community-acquired, methicillinresistantStaphylococcus aureusisolated from Chinese children

Cited by 57 publications

References 32 publications

mecA -Positive Staphylococcus aureus with Low-Level Oxacillin MIC in Taiwan

mecA -Positive Staphylococcus aureus with Low-Level Oxacillin MIC in Taiwan

Characterization of Staphylococcus aureus Strains Associated with Food Poisoning in Shenzhen, China

Microbial competition between Bacillus subtilis and Staphylococcus aureus monitored by imaging mass spectrometry

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