Molecular Characteristics of Fat Body Protein 1 in the Oriental Fruit Fly, Bactrocera dorsalis

Abstract

Bactrocera dorsails fat body protein 1 (Bdfbp1) cDNA was cloned (GenBank accession no. MT514270), and the complete 3,749-bp cDNA encoded a 1,152-amino acid protein. The phylogenetic relationship of dipteran fbp1s was analyzed. The sequence XP_028900815 from the insect genome project for Zeugodacus cucurbitae (LOC105219342) was proposed that two fbp1 genes were present in the sequence. The developmental transcriptional expression profiles were determined. In the larval stages, Bdfbp1 mRNA had significantly higher expression in the late third instar larvae compared with first, second, and early third instar larvae. In the pupal stages, the highest expression of Bdfbp1 mRNA was found in the newly pupated pupae and then decreased with age. In the fat body of female adults, Bdfbp1 was highly expressed in newly emerged samples and decreased rapidly over the following three days. In the fat body of male adults, Bdfbp1 was highly expressed in newly eclosed samples. RNAi treatment decreased the expression level of Bdfbp1 without statistical difference. However, RNAi treatment significantly decreased the rate of eclosion. These results suggest that Bdfbp1 may function as a storage protein and be associated with adult eclosion.

Transcriptomic Identification and Characterization of Trehalose-6-Phosphate Synthase in Fat Body of the Oriental Fruit Fly, Bactrocera dorsalis

“…dorsalis from various perspectives, while those on fat body of B. dorsalis were still rare events. ,− …”

Effects of photoperiod and age on the development of two types of the fat body in the Asian comma butterfly, Polygonia c-aureum (Lepidoptera: Nymphalidae), in relation to adult diapause

“…Fat bodies ll the body cavity especially in the abdominal region (Chapman 2013), and the development of fat body is a characteristic process, especially in diapausing insects. Although fat body function has been extensively studied, fat body polymorphisms have mainly been unstudied, although it has been observed that adults of the Oriental fruit y Bactrocera dorsalis (Hendel) have two types of fat body (Yu et al 2021). Also, in two lepidopteran species, two types of fat body have been identi ed that have different structures and functions (Haunerland and Shirk 1995); Larvae of the Indian meal moth Plodia interpunctella (Hubner), (Shirk and Malore 1989) and the corn earworm Helicoverpa zea (Boddie), (Haunerland et al 1990; Wang and Haunerland 1992) have two types of fat bodies, while adults have only a single type of fat body.…”